Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunos...
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Instituto Oswaldo Cruz, Ministério da Saúde
2003
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ftjscielo:oai:scielo:S0074-02762003000500017 2023-05-15T18:05:27+02:00 Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis Silva,Luciana Pereira Barcelos,Ivanildes Solange da Costa Passos-Lima,Andréia Barcelos Espindola,Foued Salmen Campos,Dulcinéa Maria Barbosa Costa-Cruz,Julia Maria 2003-07-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500017 en eng Instituto Oswaldo Cruz, Ministério da Saúde Memórias do Instituto Oswaldo Cruz v.98 n.5 2003 Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting journal article 2003 ftjscielo 2015-10-26T17:33:04Z The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. Article in Journal/Newspaper Rattus rattus SciELO Brazil (Scientific Electronic Library Online) |
institution |
Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
English |
topic |
Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting |
spellingShingle |
Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting Silva,Luciana Pereira Barcelos,Ivanildes Solange da Costa Passos-Lima,Andréia Barcelos Espindola,Foued Salmen Campos,Dulcinéa Maria Barbosa Costa-Cruz,Julia Maria Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
topic_facet |
Strongyloides stercoralis Strongyloides ratti strongyloidiasis immunodiagnosis Western blotting |
description |
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. |
format |
Article in Journal/Newspaper |
author |
Silva,Luciana Pereira Barcelos,Ivanildes Solange da Costa Passos-Lima,Andréia Barcelos Espindola,Foued Salmen Campos,Dulcinéa Maria Barbosa Costa-Cruz,Julia Maria |
author_facet |
Silva,Luciana Pereira Barcelos,Ivanildes Solange da Costa Passos-Lima,Andréia Barcelos Espindola,Foued Salmen Campos,Dulcinéa Maria Barbosa Costa-Cruz,Julia Maria |
author_sort |
Silva,Luciana Pereira |
title |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_short |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_full |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_fullStr |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_full_unstemmed |
Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis |
title_sort |
western blotting using strongyloides ratti antigen for the detection of igg antibodies as confirmatory test in human strongyloidiasis |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2003 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500017 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Memórias do Instituto Oswaldo Cruz v.98 n.5 2003 |
_version_ |
1766176915613286400 |