Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia

Little is known of the direct effects of Quaternary glaciationdeglaciation cycles in plants within southern European refugia. This study, centered in the Sierra Nevada (S Spain), used RAPD and morphometric data from 36 populations of Armeria (Plumbaginaceae) from five taxa belonging to three species...

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Published in:Anales del Jardín Botánico de Madrid
Main Authors: Fuertes Aguilar, Javier, Gutiérrez Larena, Belén, Nieto Feliner, Gonzalo
Format: Article in Journal/Newspaper
Language:English
Published: Consejo Superior de Investigaciones Científicas 2011
Subjects:
Online Access:https://rjb.revistas.csic.es/index.php/rjb/article/view/366
https://doi.org/10.3989/ajbm.2260
id ftjrjb:oai:jardinbotanico.revistas.csic.es:article/366
record_format openpolar
institution Open Polar
collection Anales del Jardín Botánico de Madrid (Real Jardín Botánico - CSIC)
op_collection_id ftjrjb
language English
topic altitudinal gradient
glacial refugia
hybridization
Iberian Peninsula
phylogeography
reticulate evolution
Sierra Nevada
gradiente altitudinal
refugios glaciales
hibridización
Península ibérica
filogeografía
evolución reticulada
spellingShingle altitudinal gradient
glacial refugia
hybridization
Iberian Peninsula
phylogeography
reticulate evolution
Sierra Nevada
gradiente altitudinal
refugios glaciales
hibridización
Península ibérica
filogeografía
evolución reticulada
Fuertes Aguilar, Javier
Gutiérrez Larena, Belén
Nieto Feliner, Gonzalo
Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia
topic_facet altitudinal gradient
glacial refugia
hybridization
Iberian Peninsula
phylogeography
reticulate evolution
Sierra Nevada
gradiente altitudinal
refugios glaciales
hibridización
Península ibérica
filogeografía
evolución reticulada
description Little is known of the direct effects of Quaternary glaciationdeglaciation cycles in plants within southern European refugia. This study, centered in the Sierra Nevada (S Spain), used RAPD and morphometric data from 36 populations of Armeria (Plumbaginaceae) from five taxa belonging to three species that are endemic to that region: A. filicaulis subsp. nevadensis, A. fili caulis subsp. trevenqueana, A. filicaulis subsp. alfacarensis, A. splendens, and A. villosa subsp. bernisii. The results based on genetic analyses at the population level (AMOVA, genetic diversity, genetic distance) and genetic and morphological analyses at individual level (haplotype phenetic distance, PCO, morphometrics) indicate that: (1) genetic diversity decreases with altitude, probably as a result of the postglacial recolonization processes, except in some secondary contact zones between taxa; (2) gene flow among interspecific populations, most likely facilitated by contraction of vegetation belts, led to the formation of hybrid taxa; (3) genetic distances among populations provide a useful basis for studying scenarios with frequent interspecific gene-flow since it allows distinguishing eventual cases of introgression from hybridogenous taxa. Poco se sabe de los efectos directos de los ciclos de glaciacióndeglaciación del Cuaternario sobre las plantas de los refugios glaciales del S de Europa. En el presente estudio, centrado en Sierra Nevada (S de España), hemos empleado RAPD y datos morfométricos de 36 poblaciones de Armeria (Plumbaginaceae) de cinco táxones pertenecientes a tres especies endémicas de esa región: A. filicaulis subsp. nevadensis, A. filicaulis subsp. trevenqueana, A. filicaulis subsp. alfacarensis, A. splendens y A. villosa subsp. bernisii. Los resultados basados en el análisis genético a nivel poblacional (AMOVA, diversidad genética, distancia genética) y los análisis genéticos y morfológicos a nivel individual (distancia fenética genotipo haploide, PCO, morfometría) indican que: (1) la diversidad genética se reduce con la altitud, probablemente como consecuencia de los procesos de colonización posgla ciales, salvo en zonas de contacto entre táxones; (2) el flujo génico entre poblaciones de distinta especie, probablemente facilitado por la contracción de los cinturones de vegetación, resultó en la formación de táxones híbridos; (3) la distancia genética entre poblaciones nos proporciona una base útil para el estudio de escenarios con frecuente flujo interespecífico de genes, ya que permite distinguir casos eventuales de introgresión de táxones hibridógenos.
format Article in Journal/Newspaper
author Fuertes Aguilar, Javier
Gutiérrez Larena, Belén
Nieto Feliner, Gonzalo
author_facet Fuertes Aguilar, Javier
Gutiérrez Larena, Belén
Nieto Feliner, Gonzalo
author_sort Fuertes Aguilar, Javier
title Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia
title_short Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia
title_full Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia
title_fullStr Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia
title_full_unstemmed Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia
title_sort genetic and morphological diversity in armeria (plumbaginaceae) is shaped by glacial cycles in mediterranean refugia
publisher Consejo Superior de Investigaciones Científicas
publishDate 2011
url https://rjb.revistas.csic.es/index.php/rjb/article/view/366
https://doi.org/10.3989/ajbm.2260
long_lat ENVELOPE(-60.783,-60.783,-63.717,-63.717)
geographic Consecuencia
geographic_facet Consecuencia
genre Arctic
genre_facet Arctic
op_source Anales del Jardín Botánico de Madrid; Vol. 68 No. 2 (2011); 175-197
Anales del Jardín Botánico de Madrid; Vol. 68 Núm. 2 (2011); 175-197
1988-3196
0211-1322
10.3989/ajbm.2011.v68.i2
op_relation https://rjb.revistas.csic.es/index.php/rjb/article/view/366/360
Aægisdóttir, H.H., Kuss & P., Stöcklin, J. 2009. Isolated populations of a rare alpine plant show high genetic diversity and considerable population differentiation. Annals of Botany 104: 1313-1322. http://dx.doi.org/10.1093/aob/mcp242 PMid:19797423 PMCid:2778390
Abbott, R.J. & Comes, H. 2004. Evolution in the Arctic: a phylogeographic analysis of the circumarctic plant, Saxifraga oppositifolia (Purple saxifrage). New Phytologist. 161: 211-224. http://dx.doi.org/10.1046/j.1469-8137.2003.00953.x
Baker, H.G. 1966. The evolution, functioning and breakdown of heteromorphic incompatibility systems. I. The Plumbaginaceae. Evolution 20: 349-368. http://dx.doi.org/10.2307/2406635
Barrón, E., Rivas-Carballo, R., Postigo-Mijarra, J.M., Alcalde-Olivares, C., Vieira, M., Castro, L., Pais, J. & Valle-Hernández, M. 2010. The Cenozoic vegetation of the Iberian Peninsula: A synthesis. Review of Palaeobotany and Palynology 162: 382-402. http://dx.doi.org/10.1016/j.revpalbo.2009.11.007
Baumbach, H. & Hellwig, F.H. 2003. Genetic variation within and among metal-tolerant and non-tolerant populations of Armeria maritima (Mill.) Willd. s.l. (Plumbaginaceae) in Central and Northeast Germany. Plant Biology 5: 186-193. http://dx.doi.org/10.1055/s-2003-40729
Blanca, G., López Onieva, M.R., Lorite, J., Martínez Lirola, M.J., Molero Mesa, J., Quintas, S., Ruiz Girela, M., Varo, M.A. & Vidal, S. 2002. Flora amenazada y endémica de Sierra Nevada., Editorial Universidad de Granada. 407 pp.
Braga, J.C., Martín, J.M. & Quesada, C. 2003. Patterns and average rates of late Neogene-Recent uplift of the Betic Cordillera, SE Spain. Geomorphology 50: 3-26. http://dx.doi.org/10.1016/S0169-555X(02)00205-2
Brochmann, C., Nilsson, T. & Gabrielsen, T.M. 1996. A classic exam ple of postglacial allopolyploid speciation re-examined using RAPD markers and nucleotide sequences: Saxifraga osloensis (Saxifragaceae). Symbolae Botanicae Upsalienses 31: 75-89.
Brochmann, C., Xiang, Q.-Y., Brunsfeld, S.J., Soltis, D.E. & Soltis, P.S. 1998. Molecular evidence for polyploid origins in Saxifraga (Saxifragaceae): the narrow arctic endemic S. svalbardensis and its widespread allies. American Journal of Botany 85: 135-143. http://dx.doi.org/10.2307/2446562 PMid:21684887
Carrión, J.S. 2002. Patterns and processes of Late Quaternary environmental change in a montane region of southwestern Europe. Quaternary Science Reviews 21: 2047-2066. http://dx.doi.org/10.1016/S0277-3791(02)00010-0
Carrión, J.S., Munuera, M., Dupré, M. & Andrade, A. 2001a. Abrupt vegetation changes in the Segura Mountains of southern Spain throughout the Holocene. Journal of Ecology 89: 783-797. http://dx.doi.org/10.1046/j.0022-0477.2001.00601.x
Carrión, J.S., Riquelme, J. A., Navarro, C. & Munuera, M. 2001b. Pollen in hyaena coprolites reflects late glacial landscape in southern Spain. Palaeogeography, Palaeoclimatology, Palaeoecology 176: 193-205. http://dx.doi.org/10.1016/S0031-0182(01)00338-8
Carrión, J.S., Yll, E.I., Walker, M.J., Legaz, A.J., Chaín, C. & López, A. 2003. Glacial refugia of temperate, Mediterranean and Ibero-North African flora in south-eastern Spain: new evidence from cave pollen at two Neanderthal man sites. Global Ecology and Biogeography 12: 119-129. http://dx.doi.org/10.1046/j.1466-822X.2003.00013.x
Comes, H.P. & Kadereit, J.W. 1998. The effect of Quaternary climatic changes on plant distribution and evolution. Trends in Plant Science 3: 432-438. http://dx.doi.org/10.1016/S1360-1385(98)01327-2
Dice, L.R. 1945. Measures of the amount of ecologic association between species. Ecology 26: 297-302. http://dx.doi.org/10.2307/1932409
Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
Evanno, G., Regnaut, S. & Goudet, J. 2005. Detecting the number of clusters of individuals using the software Structure: a simulation study. Molecular Ecology 14: 2611-2620. http://dx.doi.org/10.1111/j.1365-294X.2005.02553.x PMid:15969739
Falush, D., Stephens, M. & Pritchard, J.K. 2007.Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology Notes 7: 574-578. http://dx.doi.org/10.1111/j.1471-8286.2007.01758.x PMid:18784791 PMCid:1974779
Ferris, C., King, R.A. & Hewitt, G.M. 1999. Isolation within species and the history of glacial refugia. In: Hollingsworth P.M., Bateman R.M. & Gornall, J. (eds.), Molecular systematics and plant evolution: 20-34. Taylor and Francis, London.
Fuertes Aguilar, J. & Nieto Feliner, G 2003. Additive polymorphisms and reticulation in an ITS phylogeny of thrifts (Armeria, Plumbaginaceae). Molecular Phylogenetics and Evolution 28: 430-447. http://dx.doi.org/10.1016/S1055-7903(02)00301-9
Fuertes Aguilar, J., Rosselló, J.A. & Nieto Feliner, G. 1999. Molecular evidence for the compilospecies model of reticulate evolution in Armeria (Plumbaginaceae). Systematic Biology 48: 735-754. http://dx.doi.org/10.1080/106351599259997
Gabrielsen, T. M., Bachmann, K., Jakobson, K.S. & Brochmann, C. 1997. Glacial survival does not matter: RAPD phylogeography of Nordic Saxifraga oppositifolia. Molecular Ecology 6: 831-842. http://dx.doi.org/10.1111/j.1365-294X.1997.tb00137.x
Golan-Goldhirsh, A., Barazani, O., Wang, Z.S., Khadka, D.K., Saunders, J.A., Kostiukovsky, V. & Rowland L. J. 2004. Genetic relationships among Mediterranean Pistacia species evaluated by RAPD and AFLP markers. Plant Systematics and Evolution 246: 9-18. http://dx.doi.org/10.1007/s00606-004-0132-4
Gómez Ortiz, A. & Savador i Franch, F. 1996. Acerca de la génesis y morfodinámica del glaciarismo de Sierra Nevada. In: Chacón J. & Rosua, J.L. (eds.), I Conferencia Internacional sobre Sierra Nevada, Granada. 1, pp. 233-260.
Günster, N. & Skowronek, A. 2001. Sediment-soil sequences in the Granada Basin as evidence for long- and short-term climatic changes during the Pliocene and Quaternary in the Western Mediterranean. Quaternary International 78: 17-32. http://dx.doi.org/10.1016/S1040-6182(00)00112-9
Gutiérrez Larena, B., Fuertes Aguilar, J. & Nieto Feliner, G. 2002. Glacial-induced altitudinal migrations in Armeria (Plumbaginaceae) inferred from patterns of chloroplast DNA haplotype sharing. Molecular Ecology 11: 1965-1974. http://dx.doi.org/10.1046/j.1365-294X.2002.01594.x PMid:12296941
Gutiérrez Larena, B., Fuertes Aguilar, J. & Nieto Feliner, G. 2004. Morphometric and molecular evidence for taxonomic recognition of a new subspecies of Armeria filicaulis (Plumbaginaceae). Anales del Jardín Botánico de Madrid 61: 35-48
Gutiérrez Larena, B., Fuertes Aguilar, J. & Nieto Feliner, G. 2006. Dispersal across Southern Iberian refugia? Integrating RAPDs, sequence data and morphometrics in Armeria (Plumbaginaceae). Folia Geobotanica 41: 305-322. http://dx.doi.org/10.1007/BF02904944
Harlan, J.R. & De Wet J.M.J. 1963. The compilospecies concept. Evolution 17: 497–501. http://dx.doi.org/10.2307/2407101
Hewitt, G.M. 1996. Some genetic consequences of ice ages, and their role in divergence and speciation. Biological Journal of the Linnean Society 58: 247-276.
Hewitt, G.M. 1999. Post-glacial recolonization of European biota. Biological Journal of the Linnean Society 68: 87-112. http://dx.doi.org/10.1111/j.1095-8312.1999.tb01160.x
Hewitt, G.M. 2000. The genetic legacy of the Quaternary ice ages. Nature 405: 907-913. http://dx.doi.org/10.1038/35016000 PMid:10879524
Holderegger, R., Stehlik, I. & Abbott, R.J. 2002. Molecular analysis of the Pleistocene history of Saxifraga oppositifolia in the Alps. Molecular Ecology 11: 1409-1418. http://dx.doi.org/10.1046/j.1365-294X.2002.01548.x PMid:12144661
Hubisz, M.J., Falush, D., Stephens, M. & Pritchard. J.K.2009. Inferring weak population structure with the assistance of sample group information. Molecular Ecology Resources 9: 1322-1332. http://dx.doi.org/10.1111/j.1755-0998.2009.02591.x PMid:21564903
Jones, C.J., Edwards, K.J., Castaglione, S., Winfield, M.O., Sala, F., van de Wiel, C., Bredemeijer, G., Vosman, B., Matthes, M., Daly, A., Brettschneider, R., Bettini, P., Buiatti, M., Maestri, E., Malcevschi, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. & Karp, A. 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Molecular Breeding 3: 381-390. http://dx.doi.org/10.1023/A:1009612517139
Jordano, P. & Godoy, J.A. 2000. RAPD variation and population genetic structure in Prunus mahaleb (Rosaceae), an animal-dispersed tree. Molecular Ecology 9: 1293-1305. http://dx.doi.org/10.1046/j.1365-294x.2000.01009.x PMid:10972769
Kropf, M., Comes, H.P. & Kadereit, J.W. 2006. Long-distance dispersal vs vicariance: The origin and genetic diversity of alpine plants in the Spanish Sierra Nevada. New Phytologist 172: 169-184. http://dx.doi.org/10.1111/j.1469-8137.2006.01795.x PMid:16945099
Kropf, M., Comes, H.P. & Kadereit, J.W. 2008. Causes of the genetic architecture of south-west European high mountain disjuncts. Plant Ecology & Diversity 1: 217-228
Legendre, P. & Legendre, L. 1998. Numerical Ecology. 2nd English Edition. Development in Environmental Modelling 20. Elsevier.
Lobo, J.M., Castro, I. & Moreno, J.C. 2001. Spatial and environmental determinants of vascular plant species richness distribution in the Iberian Peninsula and Balearic Islands. Biological Journal of the Linnean Society 73: 233-253. http://dx.doi.org/10.1111/j.1095-8312.2001.tb01360.x
López González, G. 1990. Arenaria. In: Castroviejo, S., Laínz, M., López González G., Montserrat P., Muñoz Garmendia F., Paiva, J. & Villar, L. (eds.), Flora iberica 2: 172-224. CSIC, Madrid.
Lorenz, M., Weihe, A. & Börner, T. 1994. DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.). Theoretical and Applied Genetics 88: 775-779. http://dx.doi.org/10.1007/BF01253985
Lorite, J. & Valle, F. 1999. Bases para la elaboración de modelos de gestión de la vegetación en la Sierra Nevada almeriense. Conclusiones del Encuentro Medioambiental Almeriense. Universidad de Almería. 114 pp.
Martin, L.J. & Cruzan, M.B. 1999. Patterns of hybridization in the Piriqueta caroliniana complex in central Florida: evidence for an expanding hybrid zone. Evolution 53: 1037-1049. http://dx.doi.org/10.2307/2640809
Médail, F. & Quézel, P. 1997. Hot-spots analysis for conservation of plant diversity in the Mediterranean Basin. Annals of the Missouri Botanical Garden 84: 112-127. http://dx.doi.org/10.2307/2399957
Miller, M. 1997. Tools for population genetic analysis (TFPGA) 1.3: A windows program for the analysis of allozyme and molecular population genetic data.
Molero Mesa, J. 1994. Baetic and Sub-Baetic Mountains, Spain. In: Davis S.D., Heywood V.H. & Hamilton A.C. (eds.), Centres of Plant Diversity. A Guide and Strategy for their Conservation 1: Europe, Africa, SW Asia and the Middle East: pp 55-58. IUCN Publication Unit, Cambridge.
Morgan-Richards, M. & Wolff, K. 1999. Genetic structure and differentiation of Plantago major reveals a pair of sympatric sister species. Molecular Ecology 8:1027-1036. http://dx.doi.org/10.1046/j.1365-294x.1999.00665.x
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of USA 70: 3321-3323. http://dx.doi.org/10.1073/pnas.70.12.3321
op_rights Copyright (c) 2011 Consejo Superior de Investigaciones Científicas (CSIC)
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op_rightsnorm CC-BY
op_doi https://doi.org/10.3989/ajbm.2260
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spelling ftjrjb:oai:jardinbotanico.revistas.csic.es:article/366 2023-05-15T14:28:24+02:00 Genetic and morphological diversity in Armeria (Plumbaginaceae) is shaped by glacial cycles in Mediterranean refugia La diversidad genética y morfológica en Armeria (Plumbaginaceae) se debe a los ciclos glaciales en los refugios mediterráneos Fuertes Aguilar, Javier Gutiérrez Larena, Belén Nieto Feliner, Gonzalo 2011-12-30 application/pdf https://rjb.revistas.csic.es/index.php/rjb/article/view/366 https://doi.org/10.3989/ajbm.2260 eng eng Consejo Superior de Investigaciones Científicas https://rjb.revistas.csic.es/index.php/rjb/article/view/366/360 Aægisdóttir, H.H., Kuss & P., Stöcklin, J. 2009. Isolated populations of a rare alpine plant show high genetic diversity and considerable population differentiation. Annals of Botany 104: 1313-1322. http://dx.doi.org/10.1093/aob/mcp242 PMid:19797423 PMCid:2778390 Abbott, R.J. & Comes, H. 2004. Evolution in the Arctic: a phylogeographic analysis of the circumarctic plant, Saxifraga oppositifolia (Purple saxifrage). New Phytologist. 161: 211-224. http://dx.doi.org/10.1046/j.1469-8137.2003.00953.x Baker, H.G. 1966. The evolution, functioning and breakdown of heteromorphic incompatibility systems. I. The Plumbaginaceae. Evolution 20: 349-368. http://dx.doi.org/10.2307/2406635 Barrón, E., Rivas-Carballo, R., Postigo-Mijarra, J.M., Alcalde-Olivares, C., Vieira, M., Castro, L., Pais, J. & Valle-Hernández, M. 2010. The Cenozoic vegetation of the Iberian Peninsula: A synthesis. Review of Palaeobotany and Palynology 162: 382-402. http://dx.doi.org/10.1016/j.revpalbo.2009.11.007 Baumbach, H. & Hellwig, F.H. 2003. Genetic variation within and among metal-tolerant and non-tolerant populations of Armeria maritima (Mill.) Willd. s.l. (Plumbaginaceae) in Central and Northeast Germany. Plant Biology 5: 186-193. http://dx.doi.org/10.1055/s-2003-40729 Blanca, G., López Onieva, M.R., Lorite, J., Martínez Lirola, M.J., Molero Mesa, J., Quintas, S., Ruiz Girela, M., Varo, M.A. & Vidal, S. 2002. Flora amenazada y endémica de Sierra Nevada., Editorial Universidad de Granada. 407 pp. Braga, J.C., Martín, J.M. & Quesada, C. 2003. Patterns and average rates of late Neogene-Recent uplift of the Betic Cordillera, SE Spain. Geomorphology 50: 3-26. http://dx.doi.org/10.1016/S0169-555X(02)00205-2 Brochmann, C., Nilsson, T. & Gabrielsen, T.M. 1996. A classic exam ple of postglacial allopolyploid speciation re-examined using RAPD markers and nucleotide sequences: Saxifraga osloensis (Saxifragaceae). Symbolae Botanicae Upsalienses 31: 75-89. Brochmann, C., Xiang, Q.-Y., Brunsfeld, S.J., Soltis, D.E. & Soltis, P.S. 1998. Molecular evidence for polyploid origins in Saxifraga (Saxifragaceae): the narrow arctic endemic S. svalbardensis and its widespread allies. American Journal of Botany 85: 135-143. http://dx.doi.org/10.2307/2446562 PMid:21684887 Carrión, J.S. 2002. Patterns and processes of Late Quaternary environmental change in a montane region of southwestern Europe. Quaternary Science Reviews 21: 2047-2066. http://dx.doi.org/10.1016/S0277-3791(02)00010-0 Carrión, J.S., Munuera, M., Dupré, M. & Andrade, A. 2001a. Abrupt vegetation changes in the Segura Mountains of southern Spain throughout the Holocene. Journal of Ecology 89: 783-797. http://dx.doi.org/10.1046/j.0022-0477.2001.00601.x Carrión, J.S., Riquelme, J. A., Navarro, C. & Munuera, M. 2001b. Pollen in hyaena coprolites reflects late glacial landscape in southern Spain. Palaeogeography, Palaeoclimatology, Palaeoecology 176: 193-205. http://dx.doi.org/10.1016/S0031-0182(01)00338-8 Carrión, J.S., Yll, E.I., Walker, M.J., Legaz, A.J., Chaín, C. & López, A. 2003. Glacial refugia of temperate, Mediterranean and Ibero-North African flora in south-eastern Spain: new evidence from cave pollen at two Neanderthal man sites. Global Ecology and Biogeography 12: 119-129. http://dx.doi.org/10.1046/j.1466-822X.2003.00013.x Comes, H.P. & Kadereit, J.W. 1998. The effect of Quaternary climatic changes on plant distribution and evolution. Trends in Plant Science 3: 432-438. http://dx.doi.org/10.1016/S1360-1385(98)01327-2 Dice, L.R. 1945. Measures of the amount of ecologic association between species. Ecology 26: 297-302. http://dx.doi.org/10.2307/1932409 Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15. Evanno, G., Regnaut, S. & Goudet, J. 2005. Detecting the number of clusters of individuals using the software Structure: a simulation study. Molecular Ecology 14: 2611-2620. http://dx.doi.org/10.1111/j.1365-294X.2005.02553.x PMid:15969739 Falush, D., Stephens, M. & Pritchard, J.K. 2007.Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology Notes 7: 574-578. http://dx.doi.org/10.1111/j.1471-8286.2007.01758.x PMid:18784791 PMCid:1974779 Ferris, C., King, R.A. & Hewitt, G.M. 1999. Isolation within species and the history of glacial refugia. In: Hollingsworth P.M., Bateman R.M. & Gornall, J. (eds.), Molecular systematics and plant evolution: 20-34. Taylor and Francis, London. Fuertes Aguilar, J. & Nieto Feliner, G 2003. Additive polymorphisms and reticulation in an ITS phylogeny of thrifts (Armeria, Plumbaginaceae). Molecular Phylogenetics and Evolution 28: 430-447. http://dx.doi.org/10.1016/S1055-7903(02)00301-9 Fuertes Aguilar, J., Rosselló, J.A. & Nieto Feliner, G. 1999. Molecular evidence for the compilospecies model of reticulate evolution in Armeria (Plumbaginaceae). Systematic Biology 48: 735-754. http://dx.doi.org/10.1080/106351599259997 Gabrielsen, T. M., Bachmann, K., Jakobson, K.S. & Brochmann, C. 1997. Glacial survival does not matter: RAPD phylogeography of Nordic Saxifraga oppositifolia. Molecular Ecology 6: 831-842. http://dx.doi.org/10.1111/j.1365-294X.1997.tb00137.x Golan-Goldhirsh, A., Barazani, O., Wang, Z.S., Khadka, D.K., Saunders, J.A., Kostiukovsky, V. & Rowland L. J. 2004. Genetic relationships among Mediterranean Pistacia species evaluated by RAPD and AFLP markers. Plant Systematics and Evolution 246: 9-18. http://dx.doi.org/10.1007/s00606-004-0132-4 Gómez Ortiz, A. & Savador i Franch, F. 1996. Acerca de la génesis y morfodinámica del glaciarismo de Sierra Nevada. In: Chacón J. & Rosua, J.L. (eds.), I Conferencia Internacional sobre Sierra Nevada, Granada. 1, pp. 233-260. Günster, N. & Skowronek, A. 2001. Sediment-soil sequences in the Granada Basin as evidence for long- and short-term climatic changes during the Pliocene and Quaternary in the Western Mediterranean. Quaternary International 78: 17-32. http://dx.doi.org/10.1016/S1040-6182(00)00112-9 Gutiérrez Larena, B., Fuertes Aguilar, J. & Nieto Feliner, G. 2002. Glacial-induced altitudinal migrations in Armeria (Plumbaginaceae) inferred from patterns of chloroplast DNA haplotype sharing. Molecular Ecology 11: 1965-1974. http://dx.doi.org/10.1046/j.1365-294X.2002.01594.x PMid:12296941 Gutiérrez Larena, B., Fuertes Aguilar, J. & Nieto Feliner, G. 2004. Morphometric and molecular evidence for taxonomic recognition of a new subspecies of Armeria filicaulis (Plumbaginaceae). Anales del Jardín Botánico de Madrid 61: 35-48 Gutiérrez Larena, B., Fuertes Aguilar, J. & Nieto Feliner, G. 2006. Dispersal across Southern Iberian refugia? Integrating RAPDs, sequence data and morphometrics in Armeria (Plumbaginaceae). Folia Geobotanica 41: 305-322. http://dx.doi.org/10.1007/BF02904944 Harlan, J.R. & De Wet J.M.J. 1963. The compilospecies concept. Evolution 17: 497–501. http://dx.doi.org/10.2307/2407101 Hewitt, G.M. 1996. Some genetic consequences of ice ages, and their role in divergence and speciation. Biological Journal of the Linnean Society 58: 247-276. Hewitt, G.M. 1999. Post-glacial recolonization of European biota. Biological Journal of the Linnean Society 68: 87-112. http://dx.doi.org/10.1111/j.1095-8312.1999.tb01160.x Hewitt, G.M. 2000. The genetic legacy of the Quaternary ice ages. Nature 405: 907-913. http://dx.doi.org/10.1038/35016000 PMid:10879524 Holderegger, R., Stehlik, I. & Abbott, R.J. 2002. Molecular analysis of the Pleistocene history of Saxifraga oppositifolia in the Alps. Molecular Ecology 11: 1409-1418. http://dx.doi.org/10.1046/j.1365-294X.2002.01548.x PMid:12144661 Hubisz, M.J., Falush, D., Stephens, M. & Pritchard. J.K.2009. Inferring weak population structure with the assistance of sample group information. Molecular Ecology Resources 9: 1322-1332. http://dx.doi.org/10.1111/j.1755-0998.2009.02591.x PMid:21564903 Jones, C.J., Edwards, K.J., Castaglione, S., Winfield, M.O., Sala, F., van de Wiel, C., Bredemeijer, G., Vosman, B., Matthes, M., Daly, A., Brettschneider, R., Bettini, P., Buiatti, M., Maestri, E., Malcevschi, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. & Karp, A. 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Molecular Breeding 3: 381-390. http://dx.doi.org/10.1023/A:1009612517139 Jordano, P. & Godoy, J.A. 2000. RAPD variation and population genetic structure in Prunus mahaleb (Rosaceae), an animal-dispersed tree. Molecular Ecology 9: 1293-1305. http://dx.doi.org/10.1046/j.1365-294x.2000.01009.x PMid:10972769 Kropf, M., Comes, H.P. & Kadereit, J.W. 2006. Long-distance dispersal vs vicariance: The origin and genetic diversity of alpine plants in the Spanish Sierra Nevada. New Phytologist 172: 169-184. http://dx.doi.org/10.1111/j.1469-8137.2006.01795.x PMid:16945099 Kropf, M., Comes, H.P. & Kadereit, J.W. 2008. Causes of the genetic architecture of south-west European high mountain disjuncts. Plant Ecology & Diversity 1: 217-228 Legendre, P. & Legendre, L. 1998. Numerical Ecology. 2nd English Edition. Development in Environmental Modelling 20. Elsevier. Lobo, J.M., Castro, I. & Moreno, J.C. 2001. Spatial and environmental determinants of vascular plant species richness distribution in the Iberian Peninsula and Balearic Islands. Biological Journal of the Linnean Society 73: 233-253. http://dx.doi.org/10.1111/j.1095-8312.2001.tb01360.x López González, G. 1990. Arenaria. In: Castroviejo, S., Laínz, M., López González G., Montserrat P., Muñoz Garmendia F., Paiva, J. & Villar, L. (eds.), Flora iberica 2: 172-224. CSIC, Madrid. Lorenz, M., Weihe, A. & Börner, T. 1994. DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.). Theoretical and Applied Genetics 88: 775-779. http://dx.doi.org/10.1007/BF01253985 Lorite, J. & Valle, F. 1999. Bases para la elaboración de modelos de gestión de la vegetación en la Sierra Nevada almeriense. Conclusiones del Encuentro Medioambiental Almeriense. Universidad de Almería. 114 pp. Martin, L.J. & Cruzan, M.B. 1999. Patterns of hybridization in the Piriqueta caroliniana complex in central Florida: evidence for an expanding hybrid zone. Evolution 53: 1037-1049. http://dx.doi.org/10.2307/2640809 Médail, F. & Quézel, P. 1997. Hot-spots analysis for conservation of plant diversity in the Mediterranean Basin. Annals of the Missouri Botanical Garden 84: 112-127. http://dx.doi.org/10.2307/2399957 Miller, M. 1997. Tools for population genetic analysis (TFPGA) 1.3: A windows program for the analysis of allozyme and molecular population genetic data. Molero Mesa, J. 1994. Baetic and Sub-Baetic Mountains, Spain. In: Davis S.D., Heywood V.H. & Hamilton A.C. (eds.), Centres of Plant Diversity. A Guide and Strategy for their Conservation 1: Europe, Africa, SW Asia and the Middle East: pp 55-58. IUCN Publication Unit, Cambridge. Morgan-Richards, M. & Wolff, K. 1999. Genetic structure and differentiation of Plantago major reveals a pair of sympatric sister species. Molecular Ecology 8:1027-1036. http://dx.doi.org/10.1046/j.1365-294x.1999.00665.x Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of USA 70: 3321-3323. http://dx.doi.org/10.1073/pnas.70.12.3321 Copyright (c) 2011 Consejo Superior de Investigaciones Científicas (CSIC) https://creativecommons.org/licenses/by/4.0 CC-BY Anales del Jardín Botánico de Madrid; Vol. 68 No. 2 (2011); 175-197 Anales del Jardín Botánico de Madrid; Vol. 68 Núm. 2 (2011); 175-197 1988-3196 0211-1322 10.3989/ajbm.2011.v68.i2 altitudinal gradient glacial refugia hybridization Iberian Peninsula phylogeography reticulate evolution Sierra Nevada gradiente altitudinal refugios glaciales hibridización Península ibérica filogeografía evolución reticulada info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion revisado por pares; peer-reviewed 2011 ftjrjb https://doi.org/10.3989/ajbm.2260 https://doi.org/10.3989/ajbm.2011.v68.i2 https://doi.org/10.2307/2406635 https://doi.org/10.1016/S0277-3791(02)00010-0 https://doi.org/10.2307/1932409 https://doi.org/10.1111/j.1095-8312.1999.tb01160.x https:/ 2022-01-07T08:05:11Z Little is known of the direct effects of Quaternary glaciationdeglaciation cycles in plants within southern European refugia. This study, centered in the Sierra Nevada (S Spain), used RAPD and morphometric data from 36 populations of Armeria (Plumbaginaceae) from five taxa belonging to three species that are endemic to that region: A. filicaulis subsp. nevadensis, A. fili caulis subsp. trevenqueana, A. filicaulis subsp. alfacarensis, A. splendens, and A. villosa subsp. bernisii. The results based on genetic analyses at the population level (AMOVA, genetic diversity, genetic distance) and genetic and morphological analyses at individual level (haplotype phenetic distance, PCO, morphometrics) indicate that: (1) genetic diversity decreases with altitude, probably as a result of the postglacial recolonization processes, except in some secondary contact zones between taxa; (2) gene flow among interspecific populations, most likely facilitated by contraction of vegetation belts, led to the formation of hybrid taxa; (3) genetic distances among populations provide a useful basis for studying scenarios with frequent interspecific gene-flow since it allows distinguishing eventual cases of introgression from hybridogenous taxa. Poco se sabe de los efectos directos de los ciclos de glaciacióndeglaciación del Cuaternario sobre las plantas de los refugios glaciales del S de Europa. En el presente estudio, centrado en Sierra Nevada (S de España), hemos empleado RAPD y datos morfométricos de 36 poblaciones de Armeria (Plumbaginaceae) de cinco táxones pertenecientes a tres especies endémicas de esa región: A. filicaulis subsp. nevadensis, A. filicaulis subsp. trevenqueana, A. filicaulis subsp. alfacarensis, A. splendens y A. villosa subsp. bernisii. Los resultados basados en el análisis genético a nivel poblacional (AMOVA, diversidad genética, distancia genética) y los análisis genéticos y morfológicos a nivel individual (distancia fenética genotipo haploide, PCO, morfometría) indican que: (1) la diversidad genética se reduce con la altitud, probablemente como consecuencia de los procesos de colonización posgla ciales, salvo en zonas de contacto entre táxones; (2) el flujo génico entre poblaciones de distinta especie, probablemente facilitado por la contracción de los cinturones de vegetación, resultó en la formación de táxones híbridos; (3) la distancia genética entre poblaciones nos proporciona una base útil para el estudio de escenarios con frecuente flujo interespecífico de genes, ya que permite distinguir casos eventuales de introgresión de táxones hibridógenos. Article in Journal/Newspaper Arctic Anales del Jardín Botánico de Madrid (Real Jardín Botánico - CSIC) Consecuencia ENVELOPE(-60.783,-60.783,-63.717,-63.717) Anales del Jardín Botánico de Madrid 68 2 175 197