The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epi...
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Instituto Nacional de Salud
2020
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openpolar |
institution |
Open Polar |
collection |
Biomédica - Revista del Instituto Nacional de Salud, Bogotá |
op_collection_id |
ftjrbm |
language |
English |
topic |
Giardiasis/epidemiology child daycare centers Brazil giardiasis/epidemiología niño guarderías Brasil |
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Giardiasis/epidemiology child daycare centers Brazil giardiasis/epidemiología niño guarderías Brasil Figueiredo Pacheco , Flávia Thamiris Novaes Rodrigues Silva , Renata Kelly Souza de Carvalho , Silvia Carvalho Rocha , Felipe Trindade das Chagas , Gisele Maria Chagas Gomes , Daisy da Costa-Ribeiro Junior , Hugo Medrado Ribeiro, Tereza Cristina Peixoto de Mattos , Ângela Kalabric Silva , Luciano Matos Soares , Neci Aquino Teixeira, Márcia Cristina The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
topic_facet |
Giardiasis/epidemiology child daycare centers Brazil giardiasis/epidemiología niño guarderías Brasil |
description |
Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil.Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil.Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin.Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years.Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. Introducción. Giardia duodenalis es un protozoo intestinal de gran prevalencia en los niños de los países en desarrollo. En estudios moleculares se ha evidenciado la gran diversidad genética de G. duodenalis y se han identificado los conjuntos A y B, principalmente en humanos. A pesar de su importancia, el conocimiento de la epidemiología molecular de la giardiasis humana aún es limitado en Brasil.Objetivo. Caracterizar los aislamientos ... |
format |
Article in Journal/Newspaper |
author |
Figueiredo Pacheco , Flávia Thamiris Novaes Rodrigues Silva , Renata Kelly Souza de Carvalho , Silvia Carvalho Rocha , Felipe Trindade das Chagas , Gisele Maria Chagas Gomes , Daisy da Costa-Ribeiro Junior , Hugo Medrado Ribeiro, Tereza Cristina Peixoto de Mattos , Ângela Kalabric Silva , Luciano Matos Soares , Neci Aquino Teixeira, Márcia Cristina |
author_facet |
Figueiredo Pacheco , Flávia Thamiris Novaes Rodrigues Silva , Renata Kelly Souza de Carvalho , Silvia Carvalho Rocha , Felipe Trindade das Chagas , Gisele Maria Chagas Gomes , Daisy da Costa-Ribeiro Junior , Hugo Medrado Ribeiro, Tereza Cristina Peixoto de Mattos , Ângela Kalabric Silva , Luciano Matos Soares , Neci Aquino Teixeira, Márcia Cristina |
author_sort |
Figueiredo Pacheco , Flávia Thamiris |
title |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_short |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_full |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_fullStr |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_full_unstemmed |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_sort |
predominance of giardia duodenalis aii sub-assemblage in young children from salvador, bahia, brazil |
publisher |
Instituto Nacional de Salud |
publishDate |
2020 |
url |
https://revistabiomedica.org/index.php/biomedica/article/view/5161 |
genre |
Circumpolar Health |
genre_facet |
Circumpolar Health |
op_source |
Biomedica; Vol. 40 No. 3 (2020); 557-568 Biomédica; Vol. 40 Núm. 3 (2020); 557-568 2590-7379 0120-4157 |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/5161/4578 https://revistabiomedica.org/index.php/biomedica/article/view/5161/4715 Nesti MM, Goldbaum M. Infectious diseases and daycare and preschool education. J Pediatr (Rio J). 2007;83:299-312. https://doi.org/10.4269/ajtmh.199 0.42.206 Papier K, Williams GM, Luceres-Catubig R, Ahmed F, Olveda RM, McManus DP, et al. Childhood malnutrition and parasitic helminth interactions. Clin Infec Dis. 2014;59:234-43. https://doi.org/10.1093/cid/ciu211 Yones DA, Galal LA, Abdallah AM, Zaghlol KS. Effect of enteric parasitic infection on serum trace elements and nutritional status in upper Egyptian children. Trop Parasitol. 2015;5:29-35. https://doi.org/10.4103/2229-5070.145581 Thompson RCA. The zoonotic significance and molecular epidemiology of Giardia and giardiasis. Vet Parasitol. 2004;126:15-35. https://doi.org/10.1016/j.vetpar.2004.09.008 Ryan U, Hijjawi N, Feng Y, Xiao L. Giardia: An under-reported foodborne parasite. Int J Parasitol. 2019;49:1-11. https://doi.org/10.1016/j.ijpara.2018.07.003 Thompson RCA. Giardiasis as a re-emerging infectious disease and its zoonotic potential. Int J Parasitol. 2000;30:1259-67. https://doi.org/10.1016/S0020-7519(00)00127-2 Cacciò SM, De Giacomo M, Pozio E. Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol. 2002;32:1023-30. https://doi.org/10.1016/S0020-7519(02)00068-1 Feng Y, Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev. 2011;24:110-40. https://doi.org/10.1128/CMR.00033-10 Plutzer J, Ongerth J, Karanis P. Giardia taxonomy, phylogeny and epidemiology: Facts and open questions. Int J Hyg Environ Health. 2010;213:321-33. https://doi.org/10.1016/j.ijheh.2010.06.005 Thompson RCA, Ash A. Molecular epidemiology of Giardia and Cryptosporidium infections. Infect Genet Evol. 2016;40:315-23. https://doi.org/10.1016/j.meegid.2015.09.028 Monis PT, Andrews RH, Mayrhofer G, Ey PL. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genet Evol. 2003;3:29-38. https://doi.org/10.1016/S1567-1348(02)00149-1 Ballweber LR, Xiao L, Bowman DD, Kahn G, Cama VA. Giardiasis in dogs and cats: Update on epidemiology and public health significance. Trends Parasitol. 2010;26:180-9. https://doi.org/10.1016/j.pt.2010.02.005 Sprong H, Cacciò SM, van der Giessen JWB, ZOOPNET network and partners. Identification of zoonotic genotypes of Giardia duodenalis. PLoS Negl Trop Dis. 2009;3:e558. https://doi.org/10.1371/journal.pntd.0000558 Haque R, Roy S, Kabir M, Stroup SE, Mondal D, Houpt ER. Giardia assemblage A infection and diarrhea in Bangladesh. J Infect Dis. 2005;192:2171-3. https://doi.org/10.1086/498169 Sousa MC, Morais JB, Machado JE , Poiares-da-Silva J. Genotyping of Giardia lamblia human isolates from Portugal by PCR-RFLP and sequencing. J Eukaryot Microbiol. 2006;53:174-6. https://doi.org/10.1111/j.1550-7408.2006.00221.x Karanis P, Ey PL. Characterization of axenic isolates of Giardia intestinalis established from humans and animals in Germany. Parasitol Res. 1998;84:442-9. https://doi.org/10.1007/s004360050427 Johnston AR, Gillespie TR, Rwego IB, McLachlan TLT, Kent AD, Goldberg TL. Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda. PLoS Negl Trop Dis. 2010;4:e683. https://doi.org/10.1371/journal.pntd.0000683 Skhal D, Aboualchamat G, Al Mariri A, Al Nahhas S. Prevalence of Giardia duodenalis assemblages and sub-assemblages in symptomatic patients from Damascus city and its suburbs. 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ftjrbm:oai:oai.revistabiomedica.org:article/5161 2023-06-06T11:52:50+02:00 The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Predominio del subconjunto AII de Giardia duodenalis en niños pequeños de Salvador, Bahía, Brasil Figueiredo Pacheco , Flávia Thamiris Novaes Rodrigues Silva , Renata Kelly Souza de Carvalho , Silvia Carvalho Rocha , Felipe Trindade das Chagas , Gisele Maria Chagas Gomes , Daisy da Costa-Ribeiro Junior , Hugo Medrado Ribeiro, Tereza Cristina Peixoto de Mattos , Ângela Kalabric Silva , Luciano Matos Soares , Neci Aquino Teixeira, Márcia Cristina 2020-09-01 application/pdf text/xml https://revistabiomedica.org/index.php/biomedica/article/view/5161 eng eng Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5161/4578 https://revistabiomedica.org/index.php/biomedica/article/view/5161/4715 Nesti MM, Goldbaum M. Infectious diseases and daycare and preschool education. J Pediatr (Rio J). 2007;83:299-312. https://doi.org/10.4269/ajtmh.199 0.42.206 Papier K, Williams GM, Luceres-Catubig R, Ahmed F, Olveda RM, McManus DP, et al. Childhood malnutrition and parasitic helminth interactions. Clin Infec Dis. 2014;59:234-43. https://doi.org/10.1093/cid/ciu211 Yones DA, Galal LA, Abdallah AM, Zaghlol KS. Effect of enteric parasitic infection on serum trace elements and nutritional status in upper Egyptian children. Trop Parasitol. 2015;5:29-35. https://doi.org/10.4103/2229-5070.145581 Thompson RCA. The zoonotic significance and molecular epidemiology of Giardia and giardiasis. Vet Parasitol. 2004;126:15-35. https://doi.org/10.1016/j.vetpar.2004.09.008 Ryan U, Hijjawi N, Feng Y, Xiao L. Giardia: An under-reported foodborne parasite. Int J Parasitol. 2019;49:1-11. https://doi.org/10.1016/j.ijpara.2018.07.003 Thompson RCA. Giardiasis as a re-emerging infectious disease and its zoonotic potential. Int J Parasitol. 2000;30:1259-67. https://doi.org/10.1016/S0020-7519(00)00127-2 Cacciò SM, De Giacomo M, Pozio E. Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol. 2002;32:1023-30. https://doi.org/10.1016/S0020-7519(02)00068-1 Feng Y, Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev. 2011;24:110-40. https://doi.org/10.1128/CMR.00033-10 Plutzer J, Ongerth J, Karanis P. Giardia taxonomy, phylogeny and epidemiology: Facts and open questions. Int J Hyg Environ Health. 2010;213:321-33. https://doi.org/10.1016/j.ijheh.2010.06.005 Thompson RCA, Ash A. Molecular epidemiology of Giardia and Cryptosporidium infections. Infect Genet Evol. 2016;40:315-23. https://doi.org/10.1016/j.meegid.2015.09.028 Monis PT, Andrews RH, Mayrhofer G, Ey PL. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genet Evol. 2003;3:29-38. https://doi.org/10.1016/S1567-1348(02)00149-1 Ballweber LR, Xiao L, Bowman DD, Kahn G, Cama VA. Giardiasis in dogs and cats: Update on epidemiology and public health significance. Trends Parasitol. 2010;26:180-9. https://doi.org/10.1016/j.pt.2010.02.005 Sprong H, Cacciò SM, van der Giessen JWB, ZOOPNET network and partners. Identification of zoonotic genotypes of Giardia duodenalis. PLoS Negl Trop Dis. 2009;3:e558. https://doi.org/10.1371/journal.pntd.0000558 Haque R, Roy S, Kabir M, Stroup SE, Mondal D, Houpt ER. Giardia assemblage A infection and diarrhea in Bangladesh. J Infect Dis. 2005;192:2171-3. https://doi.org/10.1086/498169 Sousa MC, Morais JB, Machado JE , Poiares-da-Silva J. Genotyping of Giardia lamblia human isolates from Portugal by PCR-RFLP and sequencing. J Eukaryot Microbiol. 2006;53:174-6. https://doi.org/10.1111/j.1550-7408.2006.00221.x Karanis P, Ey PL. Characterization of axenic isolates of Giardia intestinalis established from humans and animals in Germany. Parasitol Res. 1998;84:442-9. https://doi.org/10.1007/s004360050427 Johnston AR, Gillespie TR, Rwego IB, McLachlan TLT, Kent AD, Goldberg TL. Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda. PLoS Negl Trop Dis. 2010;4:e683. https://doi.org/10.1371/journal.pntd.0000683 Skhal D, Aboualchamat G, Al Mariri A, Al Nahhas S. Prevalence of Giardia duodenalis assemblages and sub-assemblages in symptomatic patients from Damascus city and its suburbs. Infect Genet Evol. 2017;47:155-60. https://doi.org/10.1016/j.meegid.2016.11.030 Lee MF, Auer H, Lindo JF, Walochnik J. Multilocus sequence analysis of Giardia spp. isolated from patients with diarrhea in Austria. Parasitol Res. 2017;116:477-81. https://doi.org/10.1007/s00436-016-5306-9 Mbae C, Mulinge E, Guleid F, Wainaina J, Waruru A, Njiru ZK, et al. Molecular characterization of Giardia duodenalis in children in Kenya. BMC Infect Dis. 2016;16:135. https://doi.org/10.1186/s12879-016-1436-z Osman M, El Safadi D, Cian A, Benamrouz S, Nourrisson C, Poirier P, et al. Prevalence and risk factors for intestinal protozoan infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among schoolchildren in Tripoli, Lebanon. PLoS Negl Trop Dis. 2016;10:e0004496. https://doi.org/10.1371/journal.pntd.0004496 Iqbal A, Goldfarb DM, Slinger R, Dixon BR. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in diarrhoeic patients in the Qikiqtani Region, Nunavut, Canada. Int J Circumpolar Health. 2015;74:27713. https://doi.org/10.3402/ijch.v74.27713 Fahmy HM, El-Serougi AO, El Deeb HK, Hussein HM, Abou-Seri HM, Klotz C, et al. Giardia duodenalis assemblages in Egyptian children with diarrhea. Eur J Clin Microbiol Infect Dis. 2015;34:1573-81. https://doi.org/10.1007/s10096-015-2389-7 Minvielle MC, Molina NB, Polverino D, Basualdo JA. First genotyping of Giardia lamblia from human and animal feces in Argentina, South America. Mem Inst Oswaldo Cruz. 2008;103:98-103. https://doi.org/10.1590/s0074-02762008000100015 Volotão AC, Costa-Macedo LM, Haddad FSM, Brandão A, Peralta JM, Fernandes O. Genotyping of Giardia duodenalis from human and animal samples from Brazil using betagiardin gene: A phylogenetic analysis. Acta Trop. 2007;102:10-9. https://doi.org/10.1016/j.actatropica.2007.02.010 Coradi ST, David EB, Oliveira-Sequeira TC, Ribolla PE, Carvalho TB, Guimarães S. Genotyping of Brazilian Giardia duodenalis human axenic isolates. J Venom Anim Toxins Incl Trop Dis. 2011;17:353-7. https://doi.org/10.1590/S1678-91992011000300016 Oliveira-Arbex AP, David EB, Oliveira-Sequeira TCG, Bittencourt GN, Guimarães S. 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PLOS ONE. 2015;10:e0118065. https://doi.org/10.1371/journal.pone.0118065 Derechos de autor 2020 Biomédica Biomedica; Vol. 40 No. 3 (2020); 557-568 Biomédica; Vol. 40 Núm. 3 (2020); 557-568 2590-7379 0120-4157 Giardiasis/epidemiology child daycare centers Brazil giardiasis/epidemiología niño guarderías Brasil info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftjrbm https://doi.org/10.4269/ajtmh.19910.1093/cid/ciu21110.4103/2229-5070.14558110.1016/j.vetpar.2004.09.00810.1016/j.ijpara.2018.07.00310.1016/S0020-7519(00)00127-210.1016/S0020-7519(02)00068-110.1128/CMR.00033-1010.1016/j.ijheh.2010.06.00510.1016/j.meegid.20 2023-04-14T07:46:22Z Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil.Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil.Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin.Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years.Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. Introducción. Giardia duodenalis es un protozoo intestinal de gran prevalencia en los niños de los países en desarrollo. En estudios moleculares se ha evidenciado la gran diversidad genética de G. duodenalis y se han identificado los conjuntos A y B, principalmente en humanos. A pesar de su importancia, el conocimiento de la epidemiología molecular de la giardiasis humana aún es limitado en Brasil.Objetivo. Caracterizar los aislamientos ... 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