The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil

Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epi...

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Published in:Biomédica
Main Authors: Figueiredo Pacheco , Flávia Thamiris, Novaes Rodrigues Silva , Renata Kelly, Souza de Carvalho , Silvia, Carvalho Rocha , Felipe, Trindade das Chagas , Gisele Maria, Chagas Gomes , Daisy, da Costa-Ribeiro Junior , Hugo, Medrado Ribeiro, Tereza Cristina, Peixoto de Mattos , Ângela, Kalabric Silva , Luciano, Matos Soares , Neci, Aquino Teixeira, Márcia Cristina
Format: Article in Journal/Newspaper
Language:English
Published: Instituto Nacional de Salud 2020
Subjects:
Giardiasis/epidemiology
child
daycare centers
Brazil
giardiasis/epidemiología
niño
guarderías
Brasil
Circumpolar Health
Online Access:https://revistabiomedica.org/index.php/biomedica/article/view/5161
id ftjrbm:oai:oai.revistabiomedica.org:article/5161
record_format openpolar
institution Open Polar
collection Biomédica - Revista del Instituto Nacional de Salud, Bogotá
op_collection_id ftjrbm
language English
topic Giardiasis/epidemiology
child
daycare centers
Brazil
giardiasis/epidemiología
niño
guarderías
Brasil
spellingShingle Giardiasis/epidemiology
child
daycare centers
Brazil
giardiasis/epidemiología
niño
guarderías
Brasil
Figueiredo Pacheco , Flávia Thamiris
Novaes Rodrigues Silva , Renata Kelly
Souza de Carvalho , Silvia
Carvalho Rocha , Felipe
Trindade das Chagas , Gisele Maria
Chagas Gomes , Daisy
da Costa-Ribeiro Junior , Hugo
Medrado Ribeiro, Tereza Cristina
Peixoto de Mattos , Ângela
Kalabric Silva , Luciano
Matos Soares , Neci
Aquino Teixeira, Márcia Cristina
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
topic_facet Giardiasis/epidemiology
child
daycare centers
Brazil
giardiasis/epidemiología
niño
guarderías
Brasil
description Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil.Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil.Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin.Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years.Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. Introducción. Giardia duodenalis es un protozoo intestinal de gran prevalencia en los niños de los países en desarrollo. En estudios moleculares se ha evidenciado la gran diversidad genética de G. duodenalis y se han identificado los conjuntos A y B, principalmente en humanos. A pesar de su importancia, el conocimiento de la epidemiología molecular de la giardiasis humana aún es limitado en Brasil.Objetivo. Caracterizar los aislamientos ...
format Article in Journal/Newspaper
author Figueiredo Pacheco , Flávia Thamiris
Novaes Rodrigues Silva , Renata Kelly
Souza de Carvalho , Silvia
Carvalho Rocha , Felipe
Trindade das Chagas , Gisele Maria
Chagas Gomes , Daisy
da Costa-Ribeiro Junior , Hugo
Medrado Ribeiro, Tereza Cristina
Peixoto de Mattos , Ângela
Kalabric Silva , Luciano
Matos Soares , Neci
Aquino Teixeira, Márcia Cristina
author_facet Figueiredo Pacheco , Flávia Thamiris
Novaes Rodrigues Silva , Renata Kelly
Souza de Carvalho , Silvia
Carvalho Rocha , Felipe
Trindade das Chagas , Gisele Maria
Chagas Gomes , Daisy
da Costa-Ribeiro Junior , Hugo
Medrado Ribeiro, Tereza Cristina
Peixoto de Mattos , Ângela
Kalabric Silva , Luciano
Matos Soares , Neci
Aquino Teixeira, Márcia Cristina
author_sort Figueiredo Pacheco , Flávia Thamiris
title The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
title_short The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
title_full The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
title_fullStr The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
title_full_unstemmed The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
title_sort predominance of giardia duodenalis aii sub-assemblage in young children from salvador, bahia, brazil
publisher Instituto Nacional de Salud
publishDate 2020
url https://revistabiomedica.org/index.php/biomedica/article/view/5161
genre Circumpolar Health
genre_facet Circumpolar Health
op_source Biomedica; Vol. 40 No. 3 (2020); 557-568
Biomédica; Vol. 40 Núm. 3 (2020); 557-568
2590-7379
0120-4157
op_relation https://revistabiomedica.org/index.php/biomedica/article/view/5161/4578
https://revistabiomedica.org/index.php/biomedica/article/view/5161/4715
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op_rights Derechos de autor 2020 Biomédica
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spelling ftjrbm:oai:oai.revistabiomedica.org:article/5161 2023-06-06T11:52:50+02:00 The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Predominio del subconjunto AII de Giardia duodenalis en niños pequeños de Salvador, Bahía, Brasil Figueiredo Pacheco , Flávia Thamiris Novaes Rodrigues Silva , Renata Kelly Souza de Carvalho , Silvia Carvalho Rocha , Felipe Trindade das Chagas , Gisele Maria Chagas Gomes , Daisy da Costa-Ribeiro Junior , Hugo Medrado Ribeiro, Tereza Cristina Peixoto de Mattos , Ângela Kalabric Silva , Luciano Matos Soares , Neci Aquino Teixeira, Márcia Cristina 2020-09-01 application/pdf text/xml https://revistabiomedica.org/index.php/biomedica/article/view/5161 eng eng Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5161/4578 https://revistabiomedica.org/index.php/biomedica/article/view/5161/4715 Nesti MM, Goldbaum M. Infectious diseases and daycare and preschool education. J Pediatr (Rio J). 2007;83:299-312. https://doi.org/10.4269/ajtmh.199 0.42.206 Papier K, Williams GM, Luceres-Catubig R, Ahmed F, Olveda RM, McManus DP, et al. Childhood malnutrition and parasitic helminth interactions. Clin Infec Dis. 2014;59:234-43. https://doi.org/10.1093/cid/ciu211 Yones DA, Galal LA, Abdallah AM, Zaghlol KS. Effect of enteric parasitic infection on serum trace elements and nutritional status in upper Egyptian children. Trop Parasitol. 2015;5:29-35. https://doi.org/10.4103/2229-5070.145581 Thompson RCA. The zoonotic significance and molecular epidemiology of Giardia and giardiasis. Vet Parasitol. 2004;126:15-35. https://doi.org/10.1016/j.vetpar.2004.09.008 Ryan U, Hijjawi N, Feng Y, Xiao L. Giardia: An under-reported foodborne parasite. Int J Parasitol. 2019;49:1-11. https://doi.org/10.1016/j.ijpara.2018.07.003 Thompson RCA. Giardiasis as a re-emerging infectious disease and its zoonotic potential. Int J Parasitol. 2000;30:1259-67. https://doi.org/10.1016/S0020-7519(00)00127-2 Cacciò SM, De Giacomo M, Pozio E. Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol. 2002;32:1023-30. https://doi.org/10.1016/S0020-7519(02)00068-1 Feng Y, Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev. 2011;24:110-40. https://doi.org/10.1128/CMR.00033-10 Plutzer J, Ongerth J, Karanis P. Giardia taxonomy, phylogeny and epidemiology: Facts and open questions. Int J Hyg Environ Health. 2010;213:321-33. https://doi.org/10.1016/j.ijheh.2010.06.005 Thompson RCA, Ash A. Molecular epidemiology of Giardia and Cryptosporidium infections. Infect Genet Evol. 2016;40:315-23. https://doi.org/10.1016/j.meegid.2015.09.028 Monis PT, Andrews RH, Mayrhofer G, Ey PL. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genet Evol. 2003;3:29-38. https://doi.org/10.1016/S1567-1348(02)00149-1 Ballweber LR, Xiao L, Bowman DD, Kahn G, Cama VA. Giardiasis in dogs and cats: Update on epidemiology and public health significance. Trends Parasitol. 2010;26:180-9. https://doi.org/10.1016/j.pt.2010.02.005 Sprong H, Cacciò SM, van der Giessen JWB, ZOOPNET network and partners. Identification of zoonotic genotypes of Giardia duodenalis. PLoS Negl Trop Dis. 2009;3:e558. https://doi.org/10.1371/journal.pntd.0000558 Haque R, Roy S, Kabir M, Stroup SE, Mondal D, Houpt ER. Giardia assemblage A infection and diarrhea in Bangladesh. J Infect Dis. 2005;192:2171-3. https://doi.org/10.1086/498169 Sousa MC, Morais JB, Machado JE , Poiares-da-Silva J. Genotyping of Giardia lamblia human isolates from Portugal by PCR-RFLP and sequencing. 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PLOS ONE. 2015;10:e0118065. https://doi.org/10.1371/journal.pone.0118065 Derechos de autor 2020 Biomédica Biomedica; Vol. 40 No. 3 (2020); 557-568 Biomédica; Vol. 40 Núm. 3 (2020); 557-568 2590-7379 0120-4157 Giardiasis/epidemiology child daycare centers Brazil giardiasis/epidemiología niño guarderías Brasil info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftjrbm https://doi.org/10.4269/ajtmh.19910.1093/cid/ciu21110.4103/2229-5070.14558110.1016/j.vetpar.2004.09.00810.1016/j.ijpara.2018.07.00310.1016/S0020-7519(00)00127-210.1016/S0020-7519(02)00068-110.1128/CMR.00033-1010.1016/j.ijheh.2010.06.00510.1016/j.meegid.20 2023-04-14T07:46:22Z Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil.Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil.Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin.Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years.Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. Introducción. Giardia duodenalis es un protozoo intestinal de gran prevalencia en los niños de los países en desarrollo. En estudios moleculares se ha evidenciado la gran diversidad genética de G. duodenalis y se han identificado los conjuntos A y B, principalmente en humanos. A pesar de su importancia, el conocimiento de la epidemiología molecular de la giardiasis humana aún es limitado en Brasil.Objetivo. Caracterizar los aislamientos ... Article in Journal/Newspaper Circumpolar Health Biomédica - Revista del Instituto Nacional de Salud, Bogotá Biomédica 40 3 557 568

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