Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México
Introduction: Trypanosoma cruzi is the causal agent of the American trypanosomiasis, an endemic disease in México. The commensal rodents Mus musculus and Rattus rattus are reservoirs of this parasite, which invades cardiac fibers and develops parasite nests causing various lesions. Histopathological...
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| Language: | Spanish |
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Instituto Nacional de Salud
2019
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| Online Access: | https://revistabiomedica.org/index.php/biomedica/article/view/4192 |
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ftjrbm:oai:oai.revistabiomedica.org:article/4192 |
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Open Polar |
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Biomédica - Revista del Instituto Nacional de Salud, Bogotá |
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ftjrbm |
| language |
Spanish |
| topic |
Trypanosoma cruzi mice rats ratones ratas |
| spellingShingle |
Trypanosoma cruzi mice rats ratones ratas Ucan-Euan, Fernando Hernández-Betancourt, Silvia Arjona-Torres, Madeleine Panti-May, Alonso Torres-Castro, Marco Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México |
| topic_facet |
Trypanosoma cruzi mice rats ratones ratas |
| description |
Introduction: Trypanosoma cruzi is the causal agent of the American trypanosomiasis, an endemic disease in México. The commensal rodents Mus musculus and Rattus rattus are reservoirs of this parasite, which invades cardiac fibers and develops parasite nests causing various lesions. Histopathological studies in naturally infected rodents are scarce.Objective: To describe the types and frequencies of microscopic lesions in cardiac tissue of M. musculus and R. rattus infected with T. cruzi captured in Mérida, México.Materials and methods: The rodents were captured in suburban environments of Mérida. Cardiac tissue was extracted and processed by the paraffin inclusion technique and hematoxylin and eosin stained. The observation was made with a conventional microscope and all the lesions, as well as their degree, were identified.Results: Eight tissue samples of M. musculus and seven of R. rattus were studied. Parasite nests were found in 7/15, specifically 3/8 in M. musculus and 4/7 in R. rattus. The inflammatory infiltrate was the most frequent lesion. Other lesions were: Degeneration of cardiac fibers (8/15), congestion of blood vessels (6/15), and necrosis (5/15).Discussion: The lesions we observed have been described in experimental animal models and in humans with American trypanosomiasis. The inflammatory infiltrate has been identified as the most significant lesion in humans and reservoirs in the chronic stage of the disease.Conclusion: The lesions we described are associated with T. cruzi infection, which confirms that the rodents studied are reservoirs of this parasite. Introducción. Trypanosoma cruzi es el agente causal de la tripanosomiasis americana, enfermedad endémica en México. Los roedores Mus musculus y Rattus rattus son reservorios del parásito, el cual invade las fibras cardiacas y desarrolla nidos parasitarios produciendo diversas lesiones. Los estudios histopatológicos en roedores naturalmente infectados son escasos.Objetivo. Describir los tipos y las frecuencias de las lesiones microscópicas ... |
| format |
Article in Journal/Newspaper |
| author |
Ucan-Euan, Fernando Hernández-Betancourt, Silvia Arjona-Torres, Madeleine Panti-May, Alonso Torres-Castro, Marco |
| author_facet |
Ucan-Euan, Fernando Hernández-Betancourt, Silvia Arjona-Torres, Madeleine Panti-May, Alonso Torres-Castro, Marco |
| author_sort |
Ucan-Euan, Fernando |
| title |
Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México |
| title_short |
Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México |
| title_full |
Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México |
| title_fullStr |
Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México |
| title_full_unstemmed |
Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México |
| title_sort |
histopathological study in cardiac tissue of rodents infected with trypanosoma cruzi, captured in suburbs of mérida, méxico |
| publisher |
Instituto Nacional de Salud |
| publishDate |
2019 |
| url |
https://revistabiomedica.org/index.php/biomedica/article/view/4192 |
| long_lat |
ENVELOPE(-57.950,-57.950,-63.324,-63.324) |
| geographic |
Barrios |
| geographic_facet |
Barrios |
| genre |
Rattus rattus |
| genre_facet |
Rattus rattus |
| op_source |
Biomedica; Vol. 39 No. Supl. 2 (2019): Enfermedades transmisibles en el trópico, agosto; 32-43 Biomédica; Vol. 39 Núm. Supl. 2 (2019): Enfermedades transmisibles en el trópico, agosto; 32-43 2590-7379 0120-4157 |
| op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/4192/4255 https://revistabiomedica.org/index.php/biomedica/article/view/4192/4386 López-Cancino SA, Tun-Ku E, de la Cruz-Feliz HK, Ibarra-Cerdeña CN, Izeta-Alberdi A, Pech-May A, et al. Landscape ecology of Trypanosoma cruzi in the southern Yucatán Peninsula. Acta Trop. 2015;151:58-72. https://doi.org/10.1016/j.actatropica.2015.07.021 Ramsey JM, Townsend-Peterson A, Carmona-Castro O, Moo-Llanes DA, Nakazawa Y, Butrick M, et al. Atlas of Mexican Triatominae (Reduviidae: Hemiptera) and vector transmission of Chagas disease. Mem Inst Oswaldo Cruz. 2015;110:339-52. https://doi.org/10.1590/0074-02760140404 Moncayo A, Ortiz-Yanine MI. An update on Chagas disease (human American trypanosomiasis). Ann Trop Med Parasitol. 2006;100:663-77. Leite MF, Moyer MS, Andrews NW. Expression of the mammalian calcium signaling response to Trypanosoma cruzi in Xenopus laevis oocytes. Mol Biochem Parasitol. 1998;92:1-13. https://doi.org/10.1016/S0166-6851(97)00211-9 Prata A. Clinical and epidemiological aspects of Chagas disease. Lancet Infect Dis. 2001;1:92-100. https://doi.org/10.1016/S1473-3099(01)00065-2 Carrada-Bravo T. Trypanosoma cruzi: historia natural y diagnóstico de la enfermedad de Chagas. Rev Mex Patol Clin. 2004;51:205-19. Bonney KM, Engman DM. Chagas heart disease pathogenesis: One mechanism or many? Curr Mol Med. 2008;6:510-8. https://doi.org/10.2174/156652408785748004 Postan M, Dvorak JA, McDaniel JP. Studies of Trypanosoma cruzi clones in bred mice. I. A comparison of the course of infection of C3H/HeN mice with two clones isolated from a common source. Am J Trop Med Hyg. 1983;32:497-506. https://doi.org/10.4269/ajtmh.1984.33.236 Andrade LO, Galvão LM, Meirelles MN, Chiari E, Pena SD, Macedo AM. Differential tissue tropism of Trypanosoma cruzi strains: An in vitro study. Mem Inst Oswaldo Cruz. 2010;105:834-7. https://doi.org/10.1590/S0074-02762010000600018 Rozas M, Botto-Mahan C, Coronado X, Ortiz S, Cattan PE, Solari A. Coexistence of Trypanosoma cruzi genotypes in wild and peridomestic mammals in Chile. Am J Trop Med Hyg. 2007;77:643-53. Rademaker V, Herrera HM, Raffel TR, D’Andrea PS, Freitas TPT, Abreu UG, et al. What is the role of small rodents in the transmission cycle of Trypanosoma cruzi and Trypanosoma evansi (Kinetoplastida Trypanosomatidae)? A study case in the Brazilian Pantanal. Acta Trop. 2009;111:102-7. https://doi.org/10.1016/j.actatropica.2009.02.006 Brigada AM, Doña R, Caviedes-Vidal E, Moretti E, Basso B. American Tripanosomiasis: A study on the prevalence of Trypanosoma cruzi and Trypanosoma cruzi-like organisms in wild rodents in San Luis province, Argentina. Rev Soc Bras Med Trop. 2010;43:249-53. https://doi.org/10.1590/S0037-86822010000300007 Alemán A, Guerra T, Maikis TJ, Milholland MT, Castro-Arellano I, Forstner MR, et al. The prevalence of Trypanosoma cruzi, the causal agent of Chagas disease, in Texas rodent populations. Ecohealth. 2017;14:130-143. https://doi.org/10.1007/s10393-017-1205-5 Gürtler RE, Cardinal MV. Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi. Acta Trop. 2015;151:32-50. https://doi.org/10.1016/j.actatropica.2015.05.029 Zavala-Velázquez J, Barrera-Pérez M, Rodríguez-Félix ME, Guzmán-Marín E, Ruíz-Piña H. Infection by Trypanosoma cruzi in mammals in Yucatán, México: A serological and parasitological study. Rev Inst Med Trop Sao Paulo. 1996;38:289-92. Panti-May JA, de Andrade RR, Gurubel-González Y, Palomo-Arjona E, Sodá-Tamayo L, Meza-Sulú J, et al. A survey of zoonotic pathogens carried by house mouse and black rat populations in Yucatán, México. Epidemiol Infect. 2017;145:2287-95. https://doi.org/10.1017/S0950268817001352 Panti-May JA, Hernández-Betancourt SF, Torres-Castro MA, Machaín-Williams C, Cigarroa-Toledo N, Sodá L, et al. Population characteristics of human-commensal rodents present in households from Mérida, Yucatán, México. MANTER: Journal of Parasite Biodiversity. 2016;5. https://doi.org/10.13014/K2VD6WCX Organización Panamericana de la Salud (OPS). Guía para vigilancia, prevención, control y manejo clínico de la Enfermedad de Chagas aguda transmitida por alimentos. Fecha de consulta: 10 de octubre de 2017. Disponible en: http://bvs.panalimentos.org/local/File/Guia_Enfermedad_Chagas_2009esp.pdf de Diego JA, Palau MT, Gamallo C, Penin P. Relationships between histopathological findings and phylogenetic divergence in Trypanosoma cruzi. 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ftjrbm:oai:oai.revistabiomedica.org:article/4192 2023-06-06T11:58:47+02:00 Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México Estudio histopatológico de tejido cardiaco de roedores infectados con Trypanosoma cruzi capturados en barrios suburbanos de Mérida, México Ucan-Euan, Fernando Hernández-Betancourt, Silvia Arjona-Torres, Madeleine Panti-May, Alonso Torres-Castro, Marco 2019-08-01 application/pdf text/xml https://revistabiomedica.org/index.php/biomedica/article/view/4192 spa spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/4192/4255 https://revistabiomedica.org/index.php/biomedica/article/view/4192/4386 López-Cancino SA, Tun-Ku E, de la Cruz-Feliz HK, Ibarra-Cerdeña CN, Izeta-Alberdi A, Pech-May A, et al. Landscape ecology of Trypanosoma cruzi in the southern Yucatán Peninsula. Acta Trop. 2015;151:58-72. https://doi.org/10.1016/j.actatropica.2015.07.021 Ramsey JM, Townsend-Peterson A, Carmona-Castro O, Moo-Llanes DA, Nakazawa Y, Butrick M, et al. Atlas of Mexican Triatominae (Reduviidae: Hemiptera) and vector transmission of Chagas disease. Mem Inst Oswaldo Cruz. 2015;110:339-52. https://doi.org/10.1590/0074-02760140404 Moncayo A, Ortiz-Yanine MI. An update on Chagas disease (human American trypanosomiasis). Ann Trop Med Parasitol. 2006;100:663-77. Leite MF, Moyer MS, Andrews NW. Expression of the mammalian calcium signaling response to Trypanosoma cruzi in Xenopus laevis oocytes. Mol Biochem Parasitol. 1998;92:1-13. https://doi.org/10.1016/S0166-6851(97)00211-9 Prata A. Clinical and epidemiological aspects of Chagas disease. Lancet Infect Dis. 2001;1:92-100. https://doi.org/10.1016/S1473-3099(01)00065-2 Carrada-Bravo T. Trypanosoma cruzi: historia natural y diagnóstico de la enfermedad de Chagas. Rev Mex Patol Clin. 2004;51:205-19. Bonney KM, Engman DM. Chagas heart disease pathogenesis: One mechanism or many? Curr Mol Med. 2008;6:510-8. https://doi.org/10.2174/156652408785748004 Postan M, Dvorak JA, McDaniel JP. Studies of Trypanosoma cruzi clones in bred mice. I. A comparison of the course of infection of C3H/HeN mice with two clones isolated from a common source. Am J Trop Med Hyg. 1983;32:497-506. https://doi.org/10.4269/ajtmh.1984.33.236 Andrade LO, Galvão LM, Meirelles MN, Chiari E, Pena SD, Macedo AM. Differential tissue tropism of Trypanosoma cruzi strains: An in vitro study. Mem Inst Oswaldo Cruz. 2010;105:834-7. https://doi.org/10.1590/S0074-02762010000600018 Rozas M, Botto-Mahan C, Coronado X, Ortiz S, Cattan PE, Solari A. Coexistence of Trypanosoma cruzi genotypes in wild and peridomestic mammals in Chile. Am J Trop Med Hyg. 2007;77:643-53. Rademaker V, Herrera HM, Raffel TR, D’Andrea PS, Freitas TPT, Abreu UG, et al. What is the role of small rodents in the transmission cycle of Trypanosoma cruzi and Trypanosoma evansi (Kinetoplastida Trypanosomatidae)? A study case in the Brazilian Pantanal. Acta Trop. 2009;111:102-7. https://doi.org/10.1016/j.actatropica.2009.02.006 Brigada AM, Doña R, Caviedes-Vidal E, Moretti E, Basso B. American Tripanosomiasis: A study on the prevalence of Trypanosoma cruzi and Trypanosoma cruzi-like organisms in wild rodents in San Luis province, Argentina. Rev Soc Bras Med Trop. 2010;43:249-53. https://doi.org/10.1590/S0037-86822010000300007 Alemán A, Guerra T, Maikis TJ, Milholland MT, Castro-Arellano I, Forstner MR, et al. The prevalence of Trypanosoma cruzi, the causal agent of Chagas disease, in Texas rodent populations. Ecohealth. 2017;14:130-143. https://doi.org/10.1007/s10393-017-1205-5 Gürtler RE, Cardinal MV. Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi. Acta Trop. 2015;151:32-50. https://doi.org/10.1016/j.actatropica.2015.05.029 Zavala-Velázquez J, Barrera-Pérez M, Rodríguez-Félix ME, Guzmán-Marín E, Ruíz-Piña H. Infection by Trypanosoma cruzi in mammals in Yucatán, México: A serological and parasitological study. Rev Inst Med Trop Sao Paulo. 1996;38:289-92. Panti-May JA, de Andrade RR, Gurubel-González Y, Palomo-Arjona E, Sodá-Tamayo L, Meza-Sulú J, et al. A survey of zoonotic pathogens carried by house mouse and black rat populations in Yucatán, México. Epidemiol Infect. 2017;145:2287-95. https://doi.org/10.1017/S0950268817001352 Panti-May JA, Hernández-Betancourt SF, Torres-Castro MA, Machaín-Williams C, Cigarroa-Toledo N, Sodá L, et al. Population characteristics of human-commensal rodents present in households from Mérida, Yucatán, México. MANTER: Journal of Parasite Biodiversity. 2016;5. https://doi.org/10.13014/K2VD6WCX Organización Panamericana de la Salud (OPS). Guía para vigilancia, prevención, control y manejo clínico de la Enfermedad de Chagas aguda transmitida por alimentos. Fecha de consulta: 10 de octubre de 2017. Disponible en: http://bvs.panalimentos.org/local/File/Guia_Enfermedad_Chagas_2009esp.pdf de Diego JA, Palau MT, Gamallo C, Penin P. Relationships between histopathological findings and phylogenetic divergence in Trypanosoma cruzi. Trop Med Int Health. 1998;3:222-33. https://doi.org/10.1111/j.1365-3156.1998.tb00275.x Acosta-Viana KY, Guzmán-Marín E, Jiménez-Coello M, Torres-León MA, Colín-Flores RF, Ortega-Pacheco A. Cardiac lesions in naturally infected dogs with Trypanosoma cruzi. J Agr Sci Tech. 2011;A1:932-8. Torres-Castro MA, Hernández-Betancourt SF, Torres-León MA, Puerto FI. Lesiones histológicas asociadas a la posible infección por Trypanosoma cruzi (Chagas, 1909) en corazones de roedores sinantrópicos capturados en Yucatán, México. An Biol. 2016;38:29-35. Orellana R, Islebe G, Espadas C. Presente, pasado y futuro de los climas de la Península de Yucatán. Naturaleza y sociedad en el área Maya. Pasado, presente y futuro. Primera edición. México, DF: Academia Mexicana de Ciencias, CICY; 2003. p. 37-52. Flores JS, Espejel I. Tipos de vegetación de la Península de Yucatán. Etnoflora Yucatanense, Fascículo 3. Primera edición. Mérida, México: Universidad Autónoma de Yucatán; 1994. p. 135. Hernández-Betancourt S, Cimé-Pool J, Medina-Peralta S, González-Villanueva M. Fluctuación poblacional de Ototylomys Phyllotis Merriam, 1901 (Rodentia: Muridae) en una selva mediana subcaducifolia del sur de Yucatán, México. Acta Zool Mex. 2008;24:161-77. Gaertner DJ, Hallman TM, Hankenson FC, Batchelder MA. Anesthesia and analgesia for laboratory rodents. En: Fish RE, Brown MJ, Danneman PJ, Karas AZ, editors. Anesthesia and analgesia in laboratory animals. Second edition. London: Elsevier; 2008. p. 239-97. Leary S, Underwood W, Anthony R, Cartner S, Corey D, Grandin T, et al. 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Supl. 2 (2019): Enfermedades transmisibles en el trópico, agosto; 32-43 2590-7379 0120-4157 Trypanosoma cruzi mice rats ratones ratas info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftjrbm https://doi.org/10.1016/j.actatropica.2015.07.02110.1590/0074-0276014040410.1016/S0166-6851(97)00211-910.1016/S1473-3099(01)00065-210.4269/ajtmh.1984.33.23610.1590/S0074-0276201000060001810.1590/S0037-8682201000030000710.1007/s10393-017-1205-510.1016/j.ac 2023-04-14T07:46:07Z Introduction: Trypanosoma cruzi is the causal agent of the American trypanosomiasis, an endemic disease in México. The commensal rodents Mus musculus and Rattus rattus are reservoirs of this parasite, which invades cardiac fibers and develops parasite nests causing various lesions. Histopathological studies in naturally infected rodents are scarce.Objective: To describe the types and frequencies of microscopic lesions in cardiac tissue of M. musculus and R. rattus infected with T. cruzi captured in Mérida, México.Materials and methods: The rodents were captured in suburban environments of Mérida. Cardiac tissue was extracted and processed by the paraffin inclusion technique and hematoxylin and eosin stained. The observation was made with a conventional microscope and all the lesions, as well as their degree, were identified.Results: Eight tissue samples of M. musculus and seven of R. rattus were studied. Parasite nests were found in 7/15, specifically 3/8 in M. musculus and 4/7 in R. rattus. The inflammatory infiltrate was the most frequent lesion. Other lesions were: Degeneration of cardiac fibers (8/15), congestion of blood vessels (6/15), and necrosis (5/15).Discussion: The lesions we observed have been described in experimental animal models and in humans with American trypanosomiasis. The inflammatory infiltrate has been identified as the most significant lesion in humans and reservoirs in the chronic stage of the disease.Conclusion: The lesions we described are associated with T. cruzi infection, which confirms that the rodents studied are reservoirs of this parasite. Introducción. Trypanosoma cruzi es el agente causal de la tripanosomiasis americana, enfermedad endémica en México. Los roedores Mus musculus y Rattus rattus son reservorios del parásito, el cual invade las fibras cardiacas y desarrolla nidos parasitarios produciendo diversas lesiones. Los estudios histopatológicos en roedores naturalmente infectados son escasos.Objetivo. Describir los tipos y las frecuencias de las lesiones microscópicas ... Article in Journal/Newspaper Rattus rattus Biomédica - Revista del Instituto Nacional de Salud, Bogotá Barrios ENVELOPE(-57.950,-57.950,-63.324,-63.324) Biomédica 39 Supl. 2 32 43 |