High frequency of chromosome aberrations observed in lymphocytes in postmenopausal obese women

• Published in: Biomédica
• Main Authors: Tafurt-Cardona, Yaliana, Jaramillo-Ruiz, Leidy D., Muñoz-Ordóñez, Wilson, Muñoz-Benítez, Sulma L., Sierra-Torres, Carlos H.
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: Instituto Nacional de Salud 2012
• Subjects: Obesidad; menopausia; neoplasias; inestabilidad cromosómica; aberraciones cromosómicas; Colombia; Circumpolar Health
• Online Access: https://revistabiomedica.org/index.php/biomedica/article/view/412

Introduction. Epidemiological studies indicate that obesity is associated with an increased risk of 20-25% with several types of cancer.Objective. The frequency of chromosome aberrations was evaluated in lymphocytes frompostmenopausal obese and non-obese women.Materials and methods. Twenty obese and 20 non-obese women, all post-menopause, were recruited.The groups were matched according to age (± 5 years) and place of origin. After signing the consentform, women were interviewed using a structured questionnaire, and a blood sample (5 ml) was drawninto vacutainer tubes. From each sample, lymphocyte cell cultures were established with and without mitomycin C (challenge assay). Afterwards, the frequency of chromosome aberrations were recordedfor each group and treatment. Data were analyzed using the statistical program SPSS, v. 14.0.Results. Obese women had a higher frequency of chromosome aberrations when compared with nonobesewomen. After exposing the cell cultures to mitomycin C, obese women presented an increasein the number of total chromosome aberrations in comparison to non-obese women (3.7± 0.6 vs.2.70±0.6; p=0.001).Conclusions. The higher frequency of chromosome aberrations in lymphocytes from postmenopausalobese women compared to non-obese women suggested differences in the DNA repair capacity. Thismay indicate an association between genomic instability and the higher incidence of cancer in thispopulation. Introducción. Los estudios epidemiológicos indican que la obesidad está asociada en el 25 al 30 %con varios tipos de cáncer.Objetivo. Evaluar la frecuencia de aberraciones cromosómicas en linfocitos de mujeresposmenopáusicas obesas y no obesas, mediante la prueba de reto celular (challenge assay) comobiomarcador de inestabilidad genómica.Materiales y métodos. Cuarenta mujeres posmenopáusicas fueron incluidas en el estudio (20 obesasy 20 no obesas). Los grupos fueron pareados según edad (± 5 años) y procedencia. Después de la firmavoluntaria del consentimiento informado, las mujeres fueron ...