Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma

• Published in: Biomédica
• Main Authors: Ávila, Boris Santiago, Ramírez, Carolina
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: Instituto Nacional de Salud 2017
• Subjects: Polychlorinated biphenyls; blood; plasma; chromatography; gas; mass spectrometry; chemistry; analytical; bifenilos policlorados; sangre; cromatografía de gases; espectrometría de masas; química analítica; Arctic
• Online Access: https://revistabiomedica.org/index.php/biomedica/article/view/3529

Introduction: Polychlorinated biphenyls are among the five most toxic persistent contaminants for living organisms according to the Agency for Toxic Substances and Disease Registry (ATSDR).Objective: To standardize and validate an analytical method to determine and quantify polychlorinated biphenyl indicators in samples from blood plasma by means of gas chromatography-mass spectrometry.Materials and methods: We fortified a plasma pool to do the matrix assays. Additionally, we used the NIST SRM® 1958 reference material for the veracity and intermediate accuracy assays.Results: Methodology recovery percentages ranged between 88.4 and 97.5%, and the bias was less than 20%. Detection and quantification limits were 0.04 μg/L and 0.10 μg/L, respectively, for all polychlorinated biphenyl indicators. The linearity represented by the determination coefficient (R2) varied between 0.9866 and 0.9886. Accuracy, expressed as relative standard deviation was less than 20% in all the linear work range (0.5-500 μg/L). Finally, we analyzed 115 samples from Colombian population in various zones of the country and we found 65 positive samples, from which two samples were above HBM-II (7.0 μg/L, 2XΣPCB 138, 153, 180), and two, above HBM-I (3.5 μg/L, 2XΣPCB 138, 153, 180).Conclusion: The method we developed is accurate for PCB analysis in blood plasma samples and could be used for biological surveillance of these contaminants in the Colombian population Introducción. Los bifenilos policlorados se encuentran entre los cinco contaminantes orgánicos persistentes más tóxicos para los organismos vivos, según la Agency for Toxic Substances and Disease Registry (ATSDR) de los Estados Unidos.Objetivo. Estandarizar y validar un método analítico para la determinación y cuantificación de los bifenilos policlorados indicadores en muestras de plasma sanguíneo, mediante cromatografía de gases acoplada a espectrometría de masas.Materiales y métodos. Se fortificó un pool de plasma para hacer los ensayos en la matriz. Además, se utilizó el material ...