Liverworts frequently form mycothalli on Spitsbergen in the High Arctic
Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes ampli...
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Norwegian Polar Institute
2021
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ftjpolarres:oai:journals.openacademia.net:article/7727 2023-05-15T14:50:27+02:00 Liverworts frequently form mycothalli on Spitsbergen in the High Arctic Newsham, Kevin K. Goodall-Copestake, William P. 2021-09-08 text/html application/pdf application/epub+zip text/xml https://polarresearch.net/index.php/polar/article/view/7727 https://doi.org/10.33265/polar.v40.7727 eng eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/7727/13866 https://polarresearch.net/index.php/polar/article/view/7727/13865 https://polarresearch.net/index.php/polar/article/view/7727/13868 https://polarresearch.net/index.php/polar/article/view/7727/13867 https://polarresearch.net/index.php/polar/article/view/7727/13869 https://polarresearch.net/index.php/polar/article/view/7727 doi:10.33265/polar.v40.7727 Copyright (c) 2021 Kevin K. Newsham, William P. Goodall-Copestake https://creativecommons.org/licenses/by-nd/4.0 CC-BY-ND Polar Research; Vol. 40 (2021) 1751-8369 Edaphic factors Jungermanniidae leafy liverworts Serendipita Svalbard symbiosis info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftjpolarres https://doi.org/10.33265/polar.v40.7727 2021-11-11T19:14:21Z Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes amplified from plant tissues. Microscopy showed that intracellular hyphal coils, key indicators of the symbiosis, were frequent (>40% stem length colonized) in nine species of liverwort in the families Anastrophyllaceae, Lophoziaceae, Cephaloziellaceae, Cephaloziaceae and Scapaniaceae, with hyphae occurring frequently (>40% cells occupied) in the rhizoids of 10 species in the same families. Dark septate hyphae, apparently formed by ascomycetes, were frequent on the stems of members of the Anastrophyllaceae, Cephaloziellaceae and Cephaloziaceae, and typically those growing on acidic mine tailings. Sequencing of fungalrRNA genes showed the presence of nine distinct groups (based on a 3% cut-off for ITS sequence divergence) of the basidiomyceteSerendipitain the Anastrophyllaceae and Lophoziaceae, with ordinations and correlative analyses showing the presence of the genus to be positively associated with the frequency of hyphal coils, the occurrence of which was positively associated with edaphic factors (soilδ15N value and concentrations of moisture, nitrogen, carbon and organic matter). We propose that the frequency of mycothalli in leafy liverworts on west Spitsbergen, which is an order of magnitude higher than at lower latitudes, may arise from benefits conferred by mycobionts on their hosts in the harsh environment of the High Arctic. Article in Journal/Newspaper Arctic Polar Research Svalbard Spitsbergen Polar Research (E-Journal) Arctic Svalbard Polar Research 40 |
institution |
Open Polar |
collection |
Polar Research (E-Journal) |
op_collection_id |
ftjpolarres |
language |
English |
topic |
Edaphic factors Jungermanniidae leafy liverworts Serendipita Svalbard symbiosis |
spellingShingle |
Edaphic factors Jungermanniidae leafy liverworts Serendipita Svalbard symbiosis Newsham, Kevin K. Goodall-Copestake, William P. Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
topic_facet |
Edaphic factors Jungermanniidae leafy liverworts Serendipita Svalbard symbiosis |
description |
Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes amplified from plant tissues. Microscopy showed that intracellular hyphal coils, key indicators of the symbiosis, were frequent (>40% stem length colonized) in nine species of liverwort in the families Anastrophyllaceae, Lophoziaceae, Cephaloziellaceae, Cephaloziaceae and Scapaniaceae, with hyphae occurring frequently (>40% cells occupied) in the rhizoids of 10 species in the same families. Dark septate hyphae, apparently formed by ascomycetes, were frequent on the stems of members of the Anastrophyllaceae, Cephaloziellaceae and Cephaloziaceae, and typically those growing on acidic mine tailings. Sequencing of fungalrRNA genes showed the presence of nine distinct groups (based on a 3% cut-off for ITS sequence divergence) of the basidiomyceteSerendipitain the Anastrophyllaceae and Lophoziaceae, with ordinations and correlative analyses showing the presence of the genus to be positively associated with the frequency of hyphal coils, the occurrence of which was positively associated with edaphic factors (soilδ15N value and concentrations of moisture, nitrogen, carbon and organic matter). We propose that the frequency of mycothalli in leafy liverworts on west Spitsbergen, which is an order of magnitude higher than at lower latitudes, may arise from benefits conferred by mycobionts on their hosts in the harsh environment of the High Arctic. |
format |
Article in Journal/Newspaper |
author |
Newsham, Kevin K. Goodall-Copestake, William P. |
author_facet |
Newsham, Kevin K. Goodall-Copestake, William P. |
author_sort |
Newsham, Kevin K. |
title |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_short |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_full |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_fullStr |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_full_unstemmed |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_sort |
liverworts frequently form mycothalli on spitsbergen in the high arctic |
publisher |
Norwegian Polar Institute |
publishDate |
2021 |
url |
https://polarresearch.net/index.php/polar/article/view/7727 https://doi.org/10.33265/polar.v40.7727 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Polar Research Svalbard Spitsbergen |
genre_facet |
Arctic Polar Research Svalbard Spitsbergen |
op_source |
Polar Research; Vol. 40 (2021) 1751-8369 |
op_relation |
https://polarresearch.net/index.php/polar/article/view/7727/13866 https://polarresearch.net/index.php/polar/article/view/7727/13865 https://polarresearch.net/index.php/polar/article/view/7727/13868 https://polarresearch.net/index.php/polar/article/view/7727/13867 https://polarresearch.net/index.php/polar/article/view/7727/13869 https://polarresearch.net/index.php/polar/article/view/7727 doi:10.33265/polar.v40.7727 |
op_rights |
Copyright (c) 2021 Kevin K. Newsham, William P. Goodall-Copestake https://creativecommons.org/licenses/by-nd/4.0 |
op_rightsnorm |
CC-BY-ND |
op_doi |
https://doi.org/10.33265/polar.v40.7727 |
container_title |
Polar Research |
container_volume |
40 |
_version_ |
1766321472371949568 |