Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland
Polar and subpolar regions are known for their particular vulnerability and sensitivity to the detrimental effects of non-indigenous species, which is well exemplified by the Nootka lupin (Lupinus nootkatensis) spread in Iceland. Since understanding the population and ecological genetics of invasive...
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Norwegian Polar Institute
2021
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ftjpolarres:oai:journals.openacademia.net:article/4536 2023-05-15T16:45:50+02:00 Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland Skorupski, Jakub Szenejko, Magdalena Gruba-Tabaka, Martyna Śmietana, Przemysław Panicz, Remigiusz 2021-02-17 text/html application/pdf application/epub+zip text/xml https://polarresearch.net/index.php/polar/article/view/4536 https://doi.org/10.33265/polar.v40.4536 eng eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/4536/13349 https://polarresearch.net/index.php/polar/article/view/4536/13348 https://polarresearch.net/index.php/polar/article/view/4536/13351 https://polarresearch.net/index.php/polar/article/view/4536/13350 https://polarresearch.net/index.php/polar/article/view/4536 doi:10.33265/polar.v40.4536 Copyright (c) 2021 Jakub Skorupski, Magdalena Szenejko, Martyna Gruba-Tabaka, Przemysław Śmietana, Remigiusz Panicz https://creativecommons.org/licenses/by-nd/4.0 CC-BY-ND Polar Research; Vol. 40 (2021) 1751-8369 Biological invasions genetic diversity ITS2 Lupinus nootkatensis population structure nature conservation info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftjpolarres https://doi.org/10.33265/polar.v40.4536 2021-11-11T19:14:21Z Polar and subpolar regions are known for their particular vulnerability and sensitivity to the detrimental effects of non-indigenous species, which is well exemplified by the Nootka lupin (Lupinus nootkatensis) spread in Iceland. Since understanding the population and ecological genetics of invasive alien species offers hope for counteracting harmful biological invasions, the objective of the present study was to investigate interspecific variation inL. nootkatensisin Iceland in relation to a native population in Alaska. Moreover, we aimed to assess whether internal transcribed spacer 2 (ITS2) has sufficient phylogenetic applicability for a large-scale screening of the genetic diversity of a non-indigenous population of this species. This study, which is the first attempt to investigate the genetic diversity of the Nootka lupin in Iceland, included plant samples from eight locations in Iceland and one in Alaska. The analyses included genotyping by sequencing of the 417-nucleotide fragment of the 5.8S ribosomal RNA, ITS2 and part of the large subunit ribosomal RNA (GenBank MT026578-MT026580, MT077004). The main findings showed the presence of five previously unexplained single-nucleotide polymorphisms (SNPs); however, their discriminatory power for Icelandic populations was relatively low, since polymorphism information content (PIC) values ranged from 0.0182 to 0.0526, with average heterozygosity 0.0296. Concomitantly, analysis of multilocus genotypes (MLG) revealed sufficient differences in MLGs variants and their frequency to form genotypic patterns unique for Alaskan and Icelandic populations, revealing an internal genetic structure of the studied group. The proposed SNP panel needs to be supplemented with other nuclear and organellar markers. Article in Journal/Newspaper Iceland Polar Research Alaska Polar Research (E-Journal) Polar Research 40 |
institution |
Open Polar |
collection |
Polar Research (E-Journal) |
op_collection_id |
ftjpolarres |
language |
English |
topic |
Biological invasions genetic diversity ITS2 Lupinus nootkatensis population structure nature conservation |
spellingShingle |
Biological invasions genetic diversity ITS2 Lupinus nootkatensis population structure nature conservation Skorupski, Jakub Szenejko, Magdalena Gruba-Tabaka, Martyna Śmietana, Przemysław Panicz, Remigiusz Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland |
topic_facet |
Biological invasions genetic diversity ITS2 Lupinus nootkatensis population structure nature conservation |
description |
Polar and subpolar regions are known for their particular vulnerability and sensitivity to the detrimental effects of non-indigenous species, which is well exemplified by the Nootka lupin (Lupinus nootkatensis) spread in Iceland. Since understanding the population and ecological genetics of invasive alien species offers hope for counteracting harmful biological invasions, the objective of the present study was to investigate interspecific variation inL. nootkatensisin Iceland in relation to a native population in Alaska. Moreover, we aimed to assess whether internal transcribed spacer 2 (ITS2) has sufficient phylogenetic applicability for a large-scale screening of the genetic diversity of a non-indigenous population of this species. This study, which is the first attempt to investigate the genetic diversity of the Nootka lupin in Iceland, included plant samples from eight locations in Iceland and one in Alaska. The analyses included genotyping by sequencing of the 417-nucleotide fragment of the 5.8S ribosomal RNA, ITS2 and part of the large subunit ribosomal RNA (GenBank MT026578-MT026580, MT077004). The main findings showed the presence of five previously unexplained single-nucleotide polymorphisms (SNPs); however, their discriminatory power for Icelandic populations was relatively low, since polymorphism information content (PIC) values ranged from 0.0182 to 0.0526, with average heterozygosity 0.0296. Concomitantly, analysis of multilocus genotypes (MLG) revealed sufficient differences in MLGs variants and their frequency to form genotypic patterns unique for Alaskan and Icelandic populations, revealing an internal genetic structure of the studied group. The proposed SNP panel needs to be supplemented with other nuclear and organellar markers. |
format |
Article in Journal/Newspaper |
author |
Skorupski, Jakub Szenejko, Magdalena Gruba-Tabaka, Martyna Śmietana, Przemysław Panicz, Remigiusz |
author_facet |
Skorupski, Jakub Szenejko, Magdalena Gruba-Tabaka, Martyna Śmietana, Przemysław Panicz, Remigiusz |
author_sort |
Skorupski, Jakub |
title |
Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland |
title_short |
Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland |
title_full |
Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland |
title_fullStr |
Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland |
title_full_unstemmed |
Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland |
title_sort |
inferring population structure and genetic diversity of the invasive alien nootka lupin in iceland |
publisher |
Norwegian Polar Institute |
publishDate |
2021 |
url |
https://polarresearch.net/index.php/polar/article/view/4536 https://doi.org/10.33265/polar.v40.4536 |
genre |
Iceland Polar Research Alaska |
genre_facet |
Iceland Polar Research Alaska |
op_source |
Polar Research; Vol. 40 (2021) 1751-8369 |
op_relation |
https://polarresearch.net/index.php/polar/article/view/4536/13349 https://polarresearch.net/index.php/polar/article/view/4536/13348 https://polarresearch.net/index.php/polar/article/view/4536/13351 https://polarresearch.net/index.php/polar/article/view/4536/13350 https://polarresearch.net/index.php/polar/article/view/4536 doi:10.33265/polar.v40.4536 |
op_rights |
Copyright (c) 2021 Jakub Skorupski, Magdalena Szenejko, Martyna Gruba-Tabaka, Przemysław Śmietana, Remigiusz Panicz https://creativecommons.org/licenses/by-nd/4.0 |
op_rightsnorm |
CC-BY-ND |
op_doi |
https://doi.org/10.33265/polar.v40.4536 |
container_title |
Polar Research |
container_volume |
40 |
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1766035989053046784 |