Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core
The 2537-m-deep North Greenland Eemian Ice Drilling (NEEM) core provided a first-time opportunity to perform extensive microbiological analyses on selected, recently drilled ice core samples representing different depths, ages, ice structures, deposition climates and ionic compositions. Here, we app...
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ftjpolarres:oai:journals.openacademia.net:article/3244 2024-09-09T19:42:42+00:00 Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core Miteva, Vanya Rinehold, Kaitlyn Sowers, Todd Sebastian, Aswathy Brenchley, Jean 2015-02-16 application/pdf text/html application/epub+zip application/xml https://polarresearch.net/index.php/polar/article/view/3244 https://doi.org/10.3402/polar.v34.25057 eng eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/3244/pdf_9 https://polarresearch.net/index.php/polar/article/view/3244/html_4 https://polarresearch.net/index.php/polar/article/view/3244/_5 https://polarresearch.net/index.php/polar/article/view/3244/xml_3 https://polarresearch.net/index.php/polar/article/view/3244/8495 https://polarresearch.net/index.php/polar/article/view/3244/8501 https://polarresearch.net/index.php/polar/article/view/3244 doi:10.3402/polar.v34.25057 Polar Research; Vol 34 (2015) 1751-8369 Greenland NEEM ice core indigenous microbial diversity isolates Illumina MiSeq info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2015 ftjpolarres https://doi.org/10.3402/polar.v34.25057 2024-06-20T23:33:17Z The 2537-m-deep North Greenland Eemian Ice Drilling (NEEM) core provided a first-time opportunity to perform extensive microbiological analyses on selected, recently drilled ice core samples representing different depths, ages, ice structures, deposition climates and ionic compositions. Here, we applied cultivation, small subunit (SSU) rRNA gene clone library construction and Illumina next-generation sequencing (NGS) targeting the V4–V5 region, to examine the microbial abundance, viability and diversity in five decontaminated NEEM samples from selected depths (101.2, 633.05, 643.5, 1729.75 and 2051.5 m) deposited 300–80 000 years ago. These comparisons of the indigenous glacial microbial populations in the ice samples detected significant spatial and temporal variations. Major findings include: (a) different phylogenetic diversity of isolates, dominated by Actinobacteria and fungi, compared to the culture-independent diversity, in which Proteobacteria and Firmicutes were more frequent; (b) cultivation of a novel alphaproteobacterium; (c) dominance of Cyanobacteria among the SSU rRNA gene clones from the 1729.75-m ice; (d) identification of Archaea by NGS that are rarely detected in glacial ice; (e) detection of one or two dominant but different genera among the NGS sequences from each sample; (f) finding dominance of Planococcaceae over Bacillaceae among Firmicutes in the brittle and the 2051.5-m ice. The overall beta diversity between the studied ice core samples examined at the phylum/class level for each approach showed that the population structure of the brittle ice was significantly different from the two deep clathrated ice samples and the shallow ice core.Keywords: Greenland; NEEM ice core; indigenous microbial diversity; isolates; Illumina MiSeq.To access the supplementary material for this article, please see supplementary files (in the column to the right) under Article Tools.(Published: 16 February 2015)Citation: Polar Research 2015, 34, 25057, http://dx.doi.org/10.3402/polar.v34.25057 Article in Journal/Newspaper Greenland Greenland ice core ice core North Greenland Polar Research Polar Research Greenland Polar Research 34 1 25057 |
institution |
Open Polar |
collection |
Polar Research |
op_collection_id |
ftjpolarres |
language |
English |
topic |
Greenland NEEM ice core indigenous microbial diversity isolates Illumina MiSeq |
spellingShingle |
Greenland NEEM ice core indigenous microbial diversity isolates Illumina MiSeq Miteva, Vanya Rinehold, Kaitlyn Sowers, Todd Sebastian, Aswathy Brenchley, Jean Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core |
topic_facet |
Greenland NEEM ice core indigenous microbial diversity isolates Illumina MiSeq |
description |
The 2537-m-deep North Greenland Eemian Ice Drilling (NEEM) core provided a first-time opportunity to perform extensive microbiological analyses on selected, recently drilled ice core samples representing different depths, ages, ice structures, deposition climates and ionic compositions. Here, we applied cultivation, small subunit (SSU) rRNA gene clone library construction and Illumina next-generation sequencing (NGS) targeting the V4–V5 region, to examine the microbial abundance, viability and diversity in five decontaminated NEEM samples from selected depths (101.2, 633.05, 643.5, 1729.75 and 2051.5 m) deposited 300–80 000 years ago. These comparisons of the indigenous glacial microbial populations in the ice samples detected significant spatial and temporal variations. Major findings include: (a) different phylogenetic diversity of isolates, dominated by Actinobacteria and fungi, compared to the culture-independent diversity, in which Proteobacteria and Firmicutes were more frequent; (b) cultivation of a novel alphaproteobacterium; (c) dominance of Cyanobacteria among the SSU rRNA gene clones from the 1729.75-m ice; (d) identification of Archaea by NGS that are rarely detected in glacial ice; (e) detection of one or two dominant but different genera among the NGS sequences from each sample; (f) finding dominance of Planococcaceae over Bacillaceae among Firmicutes in the brittle and the 2051.5-m ice. The overall beta diversity between the studied ice core samples examined at the phylum/class level for each approach showed that the population structure of the brittle ice was significantly different from the two deep clathrated ice samples and the shallow ice core.Keywords: Greenland; NEEM ice core; indigenous microbial diversity; isolates; Illumina MiSeq.To access the supplementary material for this article, please see supplementary files (in the column to the right) under Article Tools.(Published: 16 February 2015)Citation: Polar Research 2015, 34, 25057, http://dx.doi.org/10.3402/polar.v34.25057 |
format |
Article in Journal/Newspaper |
author |
Miteva, Vanya Rinehold, Kaitlyn Sowers, Todd Sebastian, Aswathy Brenchley, Jean |
author_facet |
Miteva, Vanya Rinehold, Kaitlyn Sowers, Todd Sebastian, Aswathy Brenchley, Jean |
author_sort |
Miteva, Vanya |
title |
Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core |
title_short |
Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core |
title_full |
Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core |
title_fullStr |
Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core |
title_full_unstemmed |
Abundance, viability and diversity of the indigenous microbial populations at different depths of the NEEM Greenland ice core |
title_sort |
abundance, viability and diversity of the indigenous microbial populations at different depths of the neem greenland ice core |
publisher |
Norwegian Polar Institute |
publishDate |
2015 |
url |
https://polarresearch.net/index.php/polar/article/view/3244 https://doi.org/10.3402/polar.v34.25057 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Greenland ice core ice core North Greenland Polar Research |
genre_facet |
Greenland Greenland ice core ice core North Greenland Polar Research |
op_source |
Polar Research; Vol 34 (2015) 1751-8369 |
op_relation |
https://polarresearch.net/index.php/polar/article/view/3244/pdf_9 https://polarresearch.net/index.php/polar/article/view/3244/html_4 https://polarresearch.net/index.php/polar/article/view/3244/_5 https://polarresearch.net/index.php/polar/article/view/3244/xml_3 https://polarresearch.net/index.php/polar/article/view/3244/8495 https://polarresearch.net/index.php/polar/article/view/3244/8501 https://polarresearch.net/index.php/polar/article/view/3244 doi:10.3402/polar.v34.25057 |
op_doi |
https://doi.org/10.3402/polar.v34.25057 |
container_title |
Polar Research |
container_volume |
34 |
container_issue |
1 |
container_start_page |
25057 |
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1809911949850312704 |