Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing
Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom...
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ftjpolarres:oai:journals.openacademia.net:article/3156 2024-09-09T19:21:47+00:00 Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja 2014-11-05 application/pdf text/html application/epub+zip text/plain https://polarresearch.net/index.php/polar/article/view/3156 https://doi.org/10.3402/polar.v33.23466 eng eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/3156/pdf_1 https://polarresearch.net/index.php/polar/article/view/3156/html https://polarresearch.net/index.php/polar/article/view/3156/epub https://polarresearch.net/index.php/polar/article/view/3156/xml https://polarresearch.net/index.php/polar/article/view/3156/7539 https://polarresearch.net/index.php/polar/article/view/3156 doi:10.3402/polar.v33.23466 Polar Research; Vol 33 (2014) 1751-8369 18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2014 ftjpolarres https://doi.org/10.3402/polar.v33.23466 2024-06-20T23:33:17Z Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faciluiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species.Keywords: 18S rDNA; Arctic Ocean; biodiversity; next-generation sequencing; flow cytometryTo access the supplementary material for this article, please see supplementary files in the column to the right (under Article Tools)(Published: 5 November 2014)Citation: Polar Research 2014, 33, 23466, http://dx.doi.org/10.3402/polar.v33.23466 Article in Journal/Newspaper Arctic Arctic Ocean Polar Research Sea ice Polar Research Arctic Arctic Ocean Polar Research 33 1 23466 |
institution |
Open Polar |
collection |
Polar Research |
op_collection_id |
ftjpolarres |
language |
English |
topic |
18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry |
spellingShingle |
18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
topic_facet |
18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry |
description |
Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faciluiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species.Keywords: 18S rDNA; Arctic Ocean; biodiversity; next-generation sequencing; flow cytometryTo access the supplementary material for this article, please see supplementary files in the column to the right (under Article Tools)(Published: 5 November 2014)Citation: Polar Research 2014, 33, 23466, http://dx.doi.org/10.3402/polar.v33.23466 |
format |
Article in Journal/Newspaper |
author |
Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja |
author_facet |
Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja |
author_sort |
Kilias, Estelle Silvia |
title |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_short |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_full |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_fullStr |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_full_unstemmed |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_sort |
insight into protist diversity in arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
publisher |
Norwegian Polar Institute |
publishDate |
2014 |
url |
https://polarresearch.net/index.php/polar/article/view/3156 https://doi.org/10.3402/polar.v33.23466 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Polar Research Sea ice |
genre_facet |
Arctic Arctic Ocean Polar Research Sea ice |
op_source |
Polar Research; Vol 33 (2014) 1751-8369 |
op_relation |
https://polarresearch.net/index.php/polar/article/view/3156/pdf_1 https://polarresearch.net/index.php/polar/article/view/3156/html https://polarresearch.net/index.php/polar/article/view/3156/epub https://polarresearch.net/index.php/polar/article/view/3156/xml https://polarresearch.net/index.php/polar/article/view/3156/7539 https://polarresearch.net/index.php/polar/article/view/3156 doi:10.3402/polar.v33.23466 |
op_doi |
https://doi.org/10.3402/polar.v33.23466 |
container_title |
Polar Research |
container_volume |
33 |
container_issue |
1 |
container_start_page |
23466 |
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1809762031875653632 |