Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation
The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the...
Published in: | Polar Research |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Norwegian Polar Institute
2013
|
Subjects: | |
Online Access: | https://polarresearch.net/index.php/polar/article/view/3117 https://doi.org/10.3402/polar.v32i0.17390 |
id |
ftjpolarres:oai:journals.openacademia.net:article/3117 |
---|---|
record_format |
openpolar |
spelling |
ftjpolarres:oai:journals.openacademia.net:article/3117 2024-09-09T19:22:19+00:00 Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation Møller, Annette K. Søborg, Ditte A. Al-Soud, Waleed Abu Sørensen, Søren J. Kroer, Niels 2013-04-25 application/pdf text/html application/zip text/plain image/jpeg https://polarresearch.net/index.php/polar/article/view/3117 https://doi.org/10.3402/polar.v32i0.17390 eng eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/3117/pdf_1 https://polarresearch.net/index.php/polar/article/view/3117/html https://polarresearch.net/index.php/polar/article/view/3117/epub https://polarresearch.net/index.php/polar/article/view/3117/7182 https://polarresearch.net/index.php/polar/article/view/3117/7183 https://polarresearch.net/index.php/polar/article/view/3117/7184 https://polarresearch.net/index.php/polar/article/view/3117/7185 https://polarresearch.net/index.php/polar/article/view/3117/7186 https://polarresearch.net/index.php/polar/article/view/3117/7187 https://polarresearch.net/index.php/polar/article/view/3117 doi:10.3402/polar.v32i0.17390 Polar Research; Vol 32 (2013) 1751-8369 Taxonomic Diversity Microbial Assemblages Bacterial Density DOC info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2013 ftjpolarres https://doi.org/10.3402/polar.v32i0.17390 2024-06-20T23:33:17Z The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the microbial assemblages was different within the snow layers and between snow and freshwater. The highest diversity was seen in snow. In the middle and top snow layers, Proteobacteria, Bacteroidetes and Cyanobacteria dominated, although Actinobacteria and Firmicutes were relatively abundant also. High numbers of chloroplasts were also observed. In the deepest snow layer, large percentages of Firmicutes and Fusobacteria were seen. In freshwater, Bacteroidetes, Actinobacteria and Verrucomicrobia were the most abundant phyla while relatively few Proteobacteria and Cyanobacteria were present. Possibly, light intensity controlled the distribution of the Cyanobacteria and algae in the snow while carbon and nitrogen fixed by these autotrophs in turn fed the heterotrophic bacteria. In the lake, a probable lower light input relative to snow resulted in low numbers of Cyanobacteria and chloroplasts and, hence, limited input of organic carbon and nitrogen to the heterotrophic bacteria. Thus, differences in the physicochemical conditions may play an important role in the processes leading to distinctive bacterial community structures in High-Arctic snow and freshwater.Keywords: Taxonomic diversity; microbial assemblages; bacterial density; DOC(Published: 25 April 2013)To access the supplementary material for this article, please see supplementary files in the column to the right (under Article Tools).Citation: Polar Research 2013, 32, 17390, http://dx.doi.org/10.3402/polar.v32i0.17390 Article in Journal/Newspaper Arctic Polar Research Sea ice Polar Research Arctic Polar Research 32 1 17390 |
institution |
Open Polar |
collection |
Polar Research |
op_collection_id |
ftjpolarres |
language |
English |
topic |
Taxonomic Diversity Microbial Assemblages Bacterial Density DOC |
spellingShingle |
Taxonomic Diversity Microbial Assemblages Bacterial Density DOC Møller, Annette K. Søborg, Ditte A. Al-Soud, Waleed Abu Sørensen, Søren J. Kroer, Niels Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
topic_facet |
Taxonomic Diversity Microbial Assemblages Bacterial Density DOC |
description |
The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the microbial assemblages was different within the snow layers and between snow and freshwater. The highest diversity was seen in snow. In the middle and top snow layers, Proteobacteria, Bacteroidetes and Cyanobacteria dominated, although Actinobacteria and Firmicutes were relatively abundant also. High numbers of chloroplasts were also observed. In the deepest snow layer, large percentages of Firmicutes and Fusobacteria were seen. In freshwater, Bacteroidetes, Actinobacteria and Verrucomicrobia were the most abundant phyla while relatively few Proteobacteria and Cyanobacteria were present. Possibly, light intensity controlled the distribution of the Cyanobacteria and algae in the snow while carbon and nitrogen fixed by these autotrophs in turn fed the heterotrophic bacteria. In the lake, a probable lower light input relative to snow resulted in low numbers of Cyanobacteria and chloroplasts and, hence, limited input of organic carbon and nitrogen to the heterotrophic bacteria. Thus, differences in the physicochemical conditions may play an important role in the processes leading to distinctive bacterial community structures in High-Arctic snow and freshwater.Keywords: Taxonomic diversity; microbial assemblages; bacterial density; DOC(Published: 25 April 2013)To access the supplementary material for this article, please see supplementary files in the column to the right (under Article Tools).Citation: Polar Research 2013, 32, 17390, http://dx.doi.org/10.3402/polar.v32i0.17390 |
format |
Article in Journal/Newspaper |
author |
Møller, Annette K. Søborg, Ditte A. Al-Soud, Waleed Abu Sørensen, Søren J. Kroer, Niels |
author_facet |
Møller, Annette K. Søborg, Ditte A. Al-Soud, Waleed Abu Sørensen, Søren J. Kroer, Niels |
author_sort |
Møller, Annette K. |
title |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_short |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_full |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_fullStr |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_full_unstemmed |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_sort |
bacterial community structure in high-arctic snow and freshwater as revealed by pyrosequencing of 16s rrna genes and cultivation |
publisher |
Norwegian Polar Institute |
publishDate |
2013 |
url |
https://polarresearch.net/index.php/polar/article/view/3117 https://doi.org/10.3402/polar.v32i0.17390 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Polar Research Sea ice |
genre_facet |
Arctic Polar Research Sea ice |
op_source |
Polar Research; Vol 32 (2013) 1751-8369 |
op_relation |
https://polarresearch.net/index.php/polar/article/view/3117/pdf_1 https://polarresearch.net/index.php/polar/article/view/3117/html https://polarresearch.net/index.php/polar/article/view/3117/epub https://polarresearch.net/index.php/polar/article/view/3117/7182 https://polarresearch.net/index.php/polar/article/view/3117/7183 https://polarresearch.net/index.php/polar/article/view/3117/7184 https://polarresearch.net/index.php/polar/article/view/3117/7185 https://polarresearch.net/index.php/polar/article/view/3117/7186 https://polarresearch.net/index.php/polar/article/view/3117/7187 https://polarresearch.net/index.php/polar/article/view/3117 doi:10.3402/polar.v32i0.17390 |
op_doi |
https://doi.org/10.3402/polar.v32i0.17390 |
container_title |
Polar Research |
container_volume |
32 |
container_issue |
1 |
container_start_page |
17390 |
_version_ |
1809762596357668864 |