Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel
Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilization (Inipol E...
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Norwegian Polar Institute
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ftjpolarres:oai:journals.openacademia.net:article/3114 2024-09-09T19:10:16+00:00 Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel Rodríguez-Blanco, Arturo Duval, Audrey Pelletier, Emilien Delille, Daniel Ghiglione, Jean-François 2013-05-09 application/pdf text/html application/epub+zip text/plain https://polarresearch.net/index.php/polar/article/view/3114 https://doi.org/10.3402/polar.v32i0.18521 eng eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/3114/pdf_1 https://polarresearch.net/index.php/polar/article/view/3114/html https://polarresearch.net/index.php/polar/article/view/3114/epub https://polarresearch.net/index.php/polar/article/view/3114/7169 https://polarresearch.net/index.php/polar/article/view/3114 doi:10.3402/polar.v32i0.18521 Polar Research; Vol 32 (2013) 1751-8369 Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2013 ftjpolarres https://doi.org/10.3402/polar.v32i0.18521 2024-06-20T23:33:17Z Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilization (Inipol EAP 22) on community structure and diversity of bacteria inhabiting Kerguelen sub-Antarctic waters were studied in crude- and diesel-amended microcosms. Dynamics of total (i.e., 16S rDNA-based) and metabolically active (i.e., 16S rRNA-based) bacterial community structure and diversity were monitored using capillary-electrophoresis single-strand conformation polymorphism. Results showed that total and active community structures were differently influenced by temperature and fertilization in the presence of hydrocarbons. Both fertilization and temperature induced changes in total community structure in the presence of crude oil and diesel. However, temperature showed a limited influence on active community structure, and fertilization induced changes in the presence of crude oil only. Simpson’s index decreased for total bacterial communities at all temperatures in the presence of crude oil and diesel, whereas a lower reduction was observed for active bacterial populations. In the presence of fertilizer, the diversity of the whole community approached control values after seven incubation weeks; this was not observed for the active bacterial community. This study evidenced qualitative differences in total and active bacterial community structures of Kerguelen seawaters in the presence of hydrocarbons and different responses relative to variation in temperature and fertilization. These factors and hydrocarbons composition have to be taken into account to understand bacterial community dynamics after an oil spill.Keywords: Oil hydrocarbons; Inipol EAP 22; temperature; 16S rDNA/rRNA; sub-Antarctic seawaterCitation: Polar Research 2013, 32, 18521, http://dx.doi.org/10.3402/polar.v32i0.18521 Article in Journal/Newspaper Antarc* Antarctic Polar Research Polar Research Antarctic Kerguelen Polar Research 32 1 18521 |
institution |
Open Polar |
collection |
Polar Research |
op_collection_id |
ftjpolarres |
language |
English |
topic |
Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater |
spellingShingle |
Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater Rodríguez-Blanco, Arturo Duval, Audrey Pelletier, Emilien Delille, Daniel Ghiglione, Jean-François Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
topic_facet |
Oil hydrocarbons Inipol EAP 22 temperature 16S rDNA/rRNA sub-Antarctic seawater |
description |
Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilization (Inipol EAP 22) on community structure and diversity of bacteria inhabiting Kerguelen sub-Antarctic waters were studied in crude- and diesel-amended microcosms. Dynamics of total (i.e., 16S rDNA-based) and metabolically active (i.e., 16S rRNA-based) bacterial community structure and diversity were monitored using capillary-electrophoresis single-strand conformation polymorphism. Results showed that total and active community structures were differently influenced by temperature and fertilization in the presence of hydrocarbons. Both fertilization and temperature induced changes in total community structure in the presence of crude oil and diesel. However, temperature showed a limited influence on active community structure, and fertilization induced changes in the presence of crude oil only. Simpson’s index decreased for total bacterial communities at all temperatures in the presence of crude oil and diesel, whereas a lower reduction was observed for active bacterial populations. In the presence of fertilizer, the diversity of the whole community approached control values after seven incubation weeks; this was not observed for the active bacterial community. This study evidenced qualitative differences in total and active bacterial community structures of Kerguelen seawaters in the presence of hydrocarbons and different responses relative to variation in temperature and fertilization. These factors and hydrocarbons composition have to be taken into account to understand bacterial community dynamics after an oil spill.Keywords: Oil hydrocarbons; Inipol EAP 22; temperature; 16S rDNA/rRNA; sub-Antarctic seawaterCitation: Polar Research 2013, 32, 18521, http://dx.doi.org/10.3402/polar.v32i0.18521 |
format |
Article in Journal/Newspaper |
author |
Rodríguez-Blanco, Arturo Duval, Audrey Pelletier, Emilien Delille, Daniel Ghiglione, Jean-François |
author_facet |
Rodríguez-Blanco, Arturo Duval, Audrey Pelletier, Emilien Delille, Daniel Ghiglione, Jean-François |
author_sort |
Rodríguez-Blanco, Arturo |
title |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_short |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_full |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_fullStr |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_full_unstemmed |
Effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-Antarctic seawater exposed to crude oil and diesel fuel |
title_sort |
effects of temperature and fertilization on the structure of total versus active bacterial communities from sub-antarctic seawater exposed to crude oil and diesel fuel |
publisher |
Norwegian Polar Institute |
publishDate |
2013 |
url |
https://polarresearch.net/index.php/polar/article/view/3114 https://doi.org/10.3402/polar.v32i0.18521 |
geographic |
Antarctic Kerguelen |
geographic_facet |
Antarctic Kerguelen |
genre |
Antarc* Antarctic Polar Research |
genre_facet |
Antarc* Antarctic Polar Research |
op_source |
Polar Research; Vol 32 (2013) 1751-8369 |
op_relation |
https://polarresearch.net/index.php/polar/article/view/3114/pdf_1 https://polarresearch.net/index.php/polar/article/view/3114/html https://polarresearch.net/index.php/polar/article/view/3114/epub https://polarresearch.net/index.php/polar/article/view/3114/7169 https://polarresearch.net/index.php/polar/article/view/3114 doi:10.3402/polar.v32i0.18521 |
op_doi |
https://doi.org/10.3402/polar.v32i0.18521 |
container_title |
Polar Research |
container_volume |
32 |
container_issue |
1 |
container_start_page |
18521 |
_version_ |
1809825005194706944 |