Histopathological lesions in mice caused by rabies virus molecular variants circulating in Mexican wildlife

• Main Authors: E.Weimersheimer, José, Loza Rubio, Elizabeth, Morales Salinas, Elizabeth
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2012
• Subjects: Rabies; Wild animals; Molecular variants; Epidemiological cycle; Spongiosis; Bovine spongiform encephalopathy; Rabia; Fauna silvestre; Variantes moleculares; Ciclo epidemiológico; Espongiosis; Encefalopatía espongiforme bovina; Lince; Lynx
• Online Access: https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1401

The purpose of this study was to know weather some molecular variants of the rabies virus detected in Mexican wildlife could produce histopathological differences in mouse central nervous system. Five 6-mouse groups were inoculated intracerebrally with three different virus variants isolated from wildlife. Group 1) lynx/fox, Group 2) vampire bat, Group 3) skunk, Group 4) challenge virus standard (CVS), Group 5) normal mouse brain (NMB). Animals in Group 4 and those inoculated with all three different isolates resulted positive to the fluorescent antibody test. Animals in Group 1 had lesions characteristic of central nervous system (CNS), such as: inflammation, non-suppurative encephalitis, moderate-to-severe perivascular lymphocytic infiltration affecting only the hippocampus and the thalamic region, intra-cytoplasmic inclusions in the neurons (Negri's corpuscles), mild microgliosis, and spongiosis-like vacuoles of different sizes in the neuropil. Animals in Groups 2 and 3 showed similar lesions, but no spongiosis or Negri's corpuscles. None of the animals showed neuronophagia. No changes were found in Group 5. Differential diagnosis with bovine spongiform encephalopathy (BSE) is recommended, since very similar lesions were observed with the fox/lynx variant. El objetivo de este estudio fue conocer si algunas de las variantes moleculares del virus de la rabia detectadas en México producí­an diferencias histopatológicas en el sistema nervioso central de ratón. Se formaron cinco grupos con seis ratones cada uno, que se inocularon por ví­a intracerebral con tres variantes de fauna silvestre. Grupo 1) lince/zorro, Grupo 2) murciélago hematófago, Grupo 3) zorrillo, Grupo 4) virus estándar de desafí­o (CVS), Grupo 5) cerebro normal de ratón (CNR). El Grupo 4 y los animales inoculados con los tres diferentes aislamientos, resultaron positivos por anticuerpos fluorescentes. El Grupo 1 presentó lesiones caracterí­sticas de inflamación en el sistema nerviosos central: encefalitis no supurativa, infiltrado linfocitario ...