Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB...
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ftjohs:oai:ojs.pkp.sfu.ca:article/385 2024-09-15T17:56:36+00:00 Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis Rajamanickam, C Rajmohan, K 2012-06-30 application/pdf https://jhs.iihr.res.in/index.php/jhs/article/view/385 https://doi.org/10.24154/jhs.v7i1.385 eng eng Society for Promotion of Horticulture https://jhs.iihr.res.in/index.php/jhs/article/view/385/252 https://jhs.iihr.res.in/index.php/jhs/article/view/385 doi:10.24154/jhs.v7i1.385 Copyright (c) 2012 C Rajamanickam, K Rajmohan (Author) https://creativecommons.org/licenses/by-nc-sa/4.0 Journal of Horticultural Sciences; Vol. 7 No. 1 (2012); 34-40 2582-4899 0973-354X Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2012 ftjohs https://doi.org/10.24154/jhs.v7i1.385 2024-07-11T23:30:44Z The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2analysis too, a similar trend was observed. Article in Journal/Newspaper Attu Journal of Horticultural Sciences Journal of Horticultural Sciences 7 1 34 40 |
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Open Polar |
collection |
Journal of Horticultural Sciences |
op_collection_id |
ftjohs |
language |
English |
topic |
Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence |
spellingShingle |
Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence Rajamanickam, C Rajmohan, K Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
topic_facet |
Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence |
description |
The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2analysis too, a similar trend was observed. |
format |
Article in Journal/Newspaper |
author |
Rajamanickam, C Rajmohan, K |
author_facet |
Rajamanickam, C Rajmohan, K |
author_sort |
Rajamanickam, C |
title |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_short |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_full |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_fullStr |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_full_unstemmed |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_sort |
diversity studies in ecotypes of banana (musa spp.) using molecular markers and d2 analysis |
publisher |
Society for Promotion of Horticulture |
publishDate |
2012 |
url |
https://jhs.iihr.res.in/index.php/jhs/article/view/385 https://doi.org/10.24154/jhs.v7i1.385 |
genre |
Attu |
genre_facet |
Attu |
op_source |
Journal of Horticultural Sciences; Vol. 7 No. 1 (2012); 34-40 2582-4899 0973-354X |
op_relation |
https://jhs.iihr.res.in/index.php/jhs/article/view/385/252 https://jhs.iihr.res.in/index.php/jhs/article/view/385 doi:10.24154/jhs.v7i1.385 |
op_rights |
Copyright (c) 2012 C Rajamanickam, K Rajmohan (Author) https://creativecommons.org/licenses/by-nc-sa/4.0 |
op_doi |
https://doi.org/10.24154/jhs.v7i1.385 |
container_title |
Journal of Horticultural Sciences |
container_volume |
7 |
container_issue |
1 |
container_start_page |
34 |
op_container_end_page |
40 |
_version_ |
1810432796325314560 |