Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae

The Siberian tick-borne typhus (STT) is the most common tick-borne rickettsiosis (TBR) in Russia, registered in 17 administrative territories of the Southern Siberia and the Far East. The aim of this study was to describe clinical picture, pathological data and results of laboratory diagnostics duri...

Full description

Bibliographic Details
Published in:Russian Journal of Infection and Immunity
Main Authors: Rudakov N.V., Samoylenko I.E., Shtrek S.V., Rudakova S.A., Kumpan L.V., Igolkina Y.P., Rar V.A., Zhirakovskaya E.V., Tkachev S.E., Tikunova N.V., Kostrykina T.V., Blokhina I.A., Lents P.A.
Other Authors: The study was partially supported by a grant from the Ministry of Education and Science of Russia (project VI.55.1.1: 0309-2016-0002)., Исследование было частично поддержано грантом Министерства образования и науки России (проект VI.55.1.1: 0309-2016-0002).
Format: Article in Journal/Newspaper
Language:Russian
Published: SPb RAACI 2021
Subjects:
Online Access:https://iimmun.ru/iimm/article/view/1597
https://doi.org/10.15789/2220-7619-CAL-1597
id ftjiii:oai:iimmun.ru:article/1597
record_format openpolar
institution Open Polar
collection Russian Journal of Infection and Immunity (Infektsiya i immunitet)
op_collection_id ftjiii
language Russian
topic Candidatus Rickettsia tarasevichiae
Rickettsia sibirica
etiological agents
rickettsioses
clinical manifestations
ixodid ticks
этиологические агенты
риккетсиозы
клинические проявления
иксодовые клещи
spellingShingle Candidatus Rickettsia tarasevichiae
Rickettsia sibirica
etiological agents
rickettsioses
clinical manifestations
ixodid ticks
этиологические агенты
риккетсиозы
клинические проявления
иксодовые клещи
Rudakov N.V.
Samoylenko I.E.
Shtrek S.V.
Rudakova S.A.
Kumpan L.V.
Igolkina Y.P.
Rar V.A.
Zhirakovskaya E.V.
Tkachev S.E.
Tikunova N.V.
Kostrykina T.V.
Blokhina I.A.
Lents P.A.
Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae
topic_facet Candidatus Rickettsia tarasevichiae
Rickettsia sibirica
etiological agents
rickettsioses
clinical manifestations
ixodid ticks
этиологические агенты
риккетсиозы
клинические проявления
иксодовые клещи
description The Siberian tick-borne typhus (STT) is the most common tick-borne rickettsiosis (TBR) in Russia, registered in 17 administrative territories of the Southern Siberia and the Far East. The aim of this study was to describe clinical picture, pathological data and results of laboratory diagnostics during fatal mixed infection caused by two Rickettsia species most common in Russia — pathogenic Rickettsia sibirica and poorly examined Candidatus R. tarasevichiae, including identification of its etiological agents. A four-year-old girl in Krasnoyarsk Krai in a hyperendemic focus of the Siberian tick-borne typhus after tick sucking revealed typical TBR symptoms (scab at the site of tick suction, fever, spotted rash, myalgia) and meningeal syndrome, which is not typical for the Siberian tick-borne typhus. The child died on the seventh day of illness. Autopsy data (hepatosplenomegaly; cerebral edema, which was the immediate cause of death) and the results of histological examination (productive vasculitis of the brain, spinal cord and skin, polymorphic cell perivascular infiltrates in the liver and lungs, serous meningitis, myeloid hyperplasia of the spleen and lymph nodes, interstitial lymphoid infiltration in the myocardium) confirmed the clinical diagnosis of tick-borne rickettsiosis. The patient’s blood and brain samples were tested for a wide range of tick-borne pathogens and enteric viruses that cause brain damage using PCR followed by sequencing of the positive samples. The DNA of Rickettsia sibirica and Candidatus Rickettsia tarasevichiae was found in both blood and brain samples. R. sibirica was identified by the nucleotide sequences of gene fragments gltA, ompA and ompB, and Candidatus R. tarasevichiae — by the gltA and ompB genes using nested PCR and sequencing. All amplified fragments were sequenced in both directions; the obtained sequences were deposited in the GenBank database under the inventory numbers MK048467–MK048475. We have not identified other tick-borne pathogens or intestinal viruses in the ...
author2 The study was partially supported by a grant from the Ministry of Education and Science of Russia (project VI.55.1.1: 0309-2016-0002).
Исследование было частично поддержано грантом Министерства образования и науки России (проект VI.55.1.1: 0309-2016-0002).
format Article in Journal/Newspaper
author Rudakov N.V.
Samoylenko I.E.
Shtrek S.V.
Rudakova S.A.
Kumpan L.V.
Igolkina Y.P.
Rar V.A.
Zhirakovskaya E.V.
Tkachev S.E.
Tikunova N.V.
Kostrykina T.V.
Blokhina I.A.
Lents P.A.
author_facet Rudakov N.V.
Samoylenko I.E.
Shtrek S.V.
Rudakova S.A.
Kumpan L.V.
Igolkina Y.P.
Rar V.A.
Zhirakovskaya E.V.
Tkachev S.E.
Tikunova N.V.
Kostrykina T.V.
Blokhina I.A.
Lents P.A.
author_sort Rudakov N.V.
title Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae
title_short Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae
title_full Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae
title_fullStr Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae
title_full_unstemmed Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae
title_sort clinical and laboratory characteristics of tick-borne rickettsiosis related to rickettsia sibirica and candidatus rickettsia tarasevichiae
publisher SPb RAACI
publishDate 2021
url https://iimmun.ru/iimm/article/view/1597
https://doi.org/10.15789/2220-7619-CAL-1597
genre Krasnoyarsk Krai
Siberia
genre_facet Krasnoyarsk Krai
Siberia
op_source Russian Journal of Infection and Immunity; Vol 11, No 6 (2021); 1173-1178
Инфекция и иммунитет; Vol 11, No 6 (2021); 1173-1178
2313-7398
2220-7619
op_relation https://iimmun.ru/iimm/article/view/1597/1368
https://iimmun.ru/iimm/article/downloadSuppFile/1597/5910
https://iimmun.ru/iimm/article/downloadSuppFile/1597/5912
https://iimmun.ru/iimm/article/downloadSuppFile/1597/6904
https://iimmun.ru/iimm/article/view/1597
doi:10.15789/2220-7619-CAL-1597
op_rights Copyright (c) 2021 Rudakov N.V., Samoylenko I.E., Shtrek S.V., Rudakova S.A., Kumpan L.V., Igolkina Y.P., Rar V.A., Zhirakovskaya E.V., Tkachev S.E., Tikunova N.V., Kostrykina T.V., Blokhina I.A., Lents P.A.
https://creativecommons.org/licenses/by/4.0
op_doi https://doi.org/10.15789/2220-7619-CAL-1597
container_title Russian Journal of Infection and Immunity
container_volume 11
container_issue 6
container_start_page 1173
op_container_end_page 1178
_version_ 1772816277531787264
spelling ftjiii:oai:iimmun.ru:article/1597 2023-07-30T04:04:42+02:00 Clinical and laboratory characteristics of tick-borne rickettsiosis related to Rickettsia sibirica and Candidatus Rickettsia tarasevichiae Клинико-лабораторная характеристика клещевого риккетсиоза, связанного с микст-инфицированием Rickettsia sibirica и Candidatus Rickettsia tarasevichiae Rudakov N.V. Samoylenko I.E. Shtrek S.V. Rudakova S.A. Kumpan L.V. Igolkina Y.P. Rar V.A. Zhirakovskaya E.V. Tkachev S.E. Tikunova N.V. Kostrykina T.V. Blokhina I.A. Lents P.A. The study was partially supported by a grant from the Ministry of Education and Science of Russia (project VI.55.1.1: 0309-2016-0002). Исследование было частично поддержано грантом Министерства образования и науки России (проект VI.55.1.1: 0309-2016-0002). 2021-07-12 application/pdf https://iimmun.ru/iimm/article/view/1597 https://doi.org/10.15789/2220-7619-CAL-1597 rus rus SPb RAACI https://iimmun.ru/iimm/article/view/1597/1368 https://iimmun.ru/iimm/article/downloadSuppFile/1597/5910 https://iimmun.ru/iimm/article/downloadSuppFile/1597/5912 https://iimmun.ru/iimm/article/downloadSuppFile/1597/6904 https://iimmun.ru/iimm/article/view/1597 doi:10.15789/2220-7619-CAL-1597 Copyright (c) 2021 Rudakov N.V., Samoylenko I.E., Shtrek S.V., Rudakova S.A., Kumpan L.V., Igolkina Y.P., Rar V.A., Zhirakovskaya E.V., Tkachev S.E., Tikunova N.V., Kostrykina T.V., Blokhina I.A., Lents P.A. https://creativecommons.org/licenses/by/4.0 Russian Journal of Infection and Immunity; Vol 11, No 6 (2021); 1173-1178 Инфекция и иммунитет; Vol 11, No 6 (2021); 1173-1178 2313-7398 2220-7619 Candidatus Rickettsia tarasevichiae Rickettsia sibirica etiological agents rickettsioses clinical manifestations ixodid ticks этиологические агенты риккетсиозы клинические проявления иксодовые клещи info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftjiii https://doi.org/10.15789/2220-7619-CAL-1597 2023-07-18T20:19:22Z The Siberian tick-borne typhus (STT) is the most common tick-borne rickettsiosis (TBR) in Russia, registered in 17 administrative territories of the Southern Siberia and the Far East. The aim of this study was to describe clinical picture, pathological data and results of laboratory diagnostics during fatal mixed infection caused by two Rickettsia species most common in Russia — pathogenic Rickettsia sibirica and poorly examined Candidatus R. tarasevichiae, including identification of its etiological agents. A four-year-old girl in Krasnoyarsk Krai in a hyperendemic focus of the Siberian tick-borne typhus after tick sucking revealed typical TBR symptoms (scab at the site of tick suction, fever, spotted rash, myalgia) and meningeal syndrome, which is not typical for the Siberian tick-borne typhus. The child died on the seventh day of illness. Autopsy data (hepatosplenomegaly; cerebral edema, which was the immediate cause of death) and the results of histological examination (productive vasculitis of the brain, spinal cord and skin, polymorphic cell perivascular infiltrates in the liver and lungs, serous meningitis, myeloid hyperplasia of the spleen and lymph nodes, interstitial lymphoid infiltration in the myocardium) confirmed the clinical diagnosis of tick-borne rickettsiosis. The patient’s blood and brain samples were tested for a wide range of tick-borne pathogens and enteric viruses that cause brain damage using PCR followed by sequencing of the positive samples. The DNA of Rickettsia sibirica and Candidatus Rickettsia tarasevichiae was found in both blood and brain samples. R. sibirica was identified by the nucleotide sequences of gene fragments gltA, ompA and ompB, and Candidatus R. tarasevichiae — by the gltA and ompB genes using nested PCR and sequencing. All amplified fragments were sequenced in both directions; the obtained sequences were deposited in the GenBank database under the inventory numbers MK048467–MK048475. We have not identified other tick-borne pathogens or intestinal viruses in the ... Article in Journal/Newspaper Krasnoyarsk Krai Siberia Russian Journal of Infection and Immunity (Infektsiya i immunitet) Russian Journal of Infection and Immunity 11 6 1173 1178