Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase

The study of the functioning of various body systems in different vertebrates is one of the key tasks of comparative physiology. Teleost fish are a large group of vertebrates, however, the mechanisms of functioning of their digestive tract have been studied little and mainly only for such a model ob...

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Main Authors: A. A. Shvetsova, G. V. Morgunova, E. A. Novoderezhkina, V. M. Potekhina, A. A. Kamensky, O. S. Tarasova, А. А. Швецова, Г. В. Моргунова, Е. А. Новодережкина, В. М. Потехина, А. А. Каменский, О. С. Тарасова
Format: Article in Journal/Newspaper
Language:Russian
Published: Lomonosov Moscow State University, School of Biology 2022
Subjects:
Rho-киназа
smooth muscle
intestine
acetylcholine
muscarinic receptors
Rho-kinase
гладкая мышца
кишечник
ацетилхолин
мускари- новые холинорецепторы
White Sea
Rho
Gadus morhua
Online Access:https://vestnik-bio-msu.elpub.ru/jour/article/view/1184
id ftjhmub:oai:oai.vestnik-bio-msu.elpub.ru:article/1184
record_format openpolar
institution Open Polar
collection Herald of Moscow University. Series 16. Biology
op_collection_id ftjhmub
language Russian
topic Rho-киназа
smooth muscle
intestine
acetylcholine
muscarinic receptors
Rho-kinase
гладкая мышца
кишечник
ацетилхолин
мускари- новые холинорецепторы
spellingShingle Rho-киназа
smooth muscle
intestine
acetylcholine
muscarinic receptors
Rho-kinase
гладкая мышца
кишечник
ацетилхолин
мускари- новые холинорецепторы
A. A. Shvetsova
G. V. Morgunova
E. A. Novoderezhkina
V. M. Potekhina
A. A. Kamensky
O. S. Tarasova
А. А. Швецова
Г. В. Моргунова
Е. А. Новодережкина
В. М. Потехина
А. А. Каменский
О. С. Тарасова
Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase
topic_facet Rho-киназа
smooth muscle
intestine
acetylcholine
muscarinic receptors
Rho-kinase
гладкая мышца
кишечник
ацетилхолин
мускари- новые холинорецепторы
description The study of the functioning of various body systems in different vertebrates is one of the key tasks of comparative physiology. Teleost fish are a large group of vertebrates, however, the mechanisms of functioning of their digestive tract have been studied little and mainly only for such a model object as Danio rerio. The aim of this work was to characterize the participation of various subtypes of M-cholinergic receptors and the enzyme Rho-kinase in the cholinergic contraction of the intestinal smooth muscles of the White Sea cod Gadus morhua marisalbi (Gadidae). A longitudinal strip was excised from the proximal cod intestine and placed in an apparatus for recording contractile responses in the isometric mode. Contractile responses to acetylcholine were completely blocked by atropine. Blockade of M3 cholinergic receptors with 4-DAMP resulted in a decrease in acetylcholine-induced contraction compared with the control response. Blockade of M1 receptors with pirenzepine led to a weakening of contraction, less pronounced than with blockade of M3 cholinergic receptors. Blockade of M2-cholinergic receptors with methoctramine did not affect the magnitude of the contractile response. Incubation of preparations with the Rho-kinase inhibitor fasudil was accompanied by a significant decrease in contractile responses compared with the control, as well as a faster decrease in the contraction force after reaching the “peak” of the reaction. Thus, acetylcholine causes contraction of cod intestinal smooth muscle by activating M3- and M1- but not M2-cholinergic receptors. The activity of the Rho-kinase enzyme contributes to the development and maintenance of cod intestinal smooth muscle contraction under the action of acetylcholine. The results obtained are of interest for comparative physiology, may be important for understanding the mechanisms of the damaging effect of environmental factors on the bony fish’ body, as well as for the use of fish as objects of preclinical studies of drugs. Изучение особенностей ...
format Article in Journal/Newspaper
author A. A. Shvetsova
G. V. Morgunova
E. A. Novoderezhkina
V. M. Potekhina
A. A. Kamensky
O. S. Tarasova
А. А. Швецова
Г. В. Моргунова
Е. А. Новодережкина
В. М. Потехина
А. А. Каменский
О. С. Тарасова
author_facet A. A. Shvetsova
G. V. Morgunova
E. A. Novoderezhkina
V. M. Potekhina
A. A. Kamensky
O. S. Tarasova
А. А. Швецова
Г. В. Моргунова
Е. А. Новодережкина
В. М. Потехина
А. А. Каменский
О. С. Тарасова
author_sort A. A. Shvetsova
title Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase
title_short Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase
title_full Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase
title_fullStr Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase
title_full_unstemmed Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase
title_sort mechanisms of the white sea cod gadus morhua marisalbi (gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of m-cholinergic receptors and rho-kinase
publisher Lomonosov Moscow State University, School of Biology
publishDate 2022
url https://vestnik-bio-msu.elpub.ru/jour/article/view/1184
long_lat ENVELOPE(-63.000,-63.000,-64.300,-64.300)
geographic White Sea
Rho
geographic_facet White Sea
Rho
genre Gadus morhua
White Sea
genre_facet Gadus morhua
White Sea
op_source Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 77, № 4 (2022); 231-240
Вестник Московского университета. Серия 16. Биология; Том 77, № 4 (2022); 231-240
0137-0952
op_relation https://vestnik-bio-msu.elpub.ru/jour/article/view/1184/604
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Cueto-Escobedo J., German-Ponciano L.J., Guillén- Ruiz G., Soria-Fregozo C., Herrera-Huerta E.V. Zebrafish as a useful tool in the research of natural products with potential anxiolytic effects // Front. Behav. Neurosci. 2022. Vol. 15: 795285.
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spelling ftjhmub:oai:oai.vestnik-bio-msu.elpub.ru:article/1184 2023-05-15T16:19:01+02:00 Mechanisms of the White Sea cod Gadus morhua marisalbi (Gadidae) intestinal smooth muscle cholinergic contraction: the contribution of various subtypes of M-cholinergic receptors and Rho-kinase Механизмы холинергического сокращения гладкой мускулатуры кишечника беломорской трески Gadus morhua marisalbi (Gadidae): вклад различных подтипов М-холинорецепторов и Rho-киназы A. A. Shvetsova G. V. Morgunova E. A. Novoderezhkina V. M. Potekhina A. A. Kamensky O. S. Tarasova А. А. Швецова Г. В. Моргунова Е. А. Новодережкина В. М. Потехина А. А. Каменский О. С. Тарасова 2022-11-29 application/pdf https://vestnik-bio-msu.elpub.ru/jour/article/view/1184 rus rus Lomonosov Moscow State University, School of Biology https://vestnik-bio-msu.elpub.ru/jour/article/view/1184/604 Shahjahan M. Taslima K., Rahman M.S., Al- Emran M., Alam S.I., Faggio С. Effects of heavy metals on fish physiology – A review // Chemosphere. 2022. Vol. 300: 134519. Pasparakis C., Esbaughb A.J., Burggrenc W., Grosella M. Impacts of deepwater horizon oil on fish // Comp. Biochem. Physiol. Part – C: Toxicol. Pharmacol. 2019. Vol. 224: 108558. Cueto-Escobedo J., German-Ponciano L.J., Guillén- Ruiz G., Soria-Fregozo C., Herrera-Huerta E.V. Zebrafish as a useful tool in the research of natural products with potential anxiolytic effects // Front. Behav. Neurosci. 2022. Vol. 15: 795285. Karila P., Shahbazi F., Jensen J., Holmgren S. Projections and actions of tachykininergic, cholinergic, and serotonergic neurones in the intestine of the Atlantic cod // Cell Tissue Res. 1998. Vol. 291. N 3. P. 403–413. Olsson C., Holmgren S. Autonomic control of gut motility: A comparative view // Auton. Neurosci. Basic Clin. 2011. Vol. 165. N 1. P. 80–101. Jensen J., Holmgren S. Neurotransmitters in the intestine of the atlantic cod, Gadus morhua // Comp. Biochem. Physiol. Part – C: Toxicol. Pharmacol. 1985. Vol. 82. N 1. P. 81–89. Gómez A., Martos F., Bellido I., Marquez E., Garcia A. J., Pavia J., de la Cuesta F.S. Muscarinic receptor subtypes in human and rat colon smooth muscle // Biochem. Pharmacol. 1992. Vol. 43. N 11. P. 2413–2419. Unno T., Matsuyama H., Sakamoto T., Uchiyama M., Izumi Y., Okamoto H., Yamada M., Wess J., Komori S. M2 and M3 muscarinic receptor-mediated contractions in longitudinal smooth muscle of the ileum studied with receptor knockout mice // Br. J. Pharmacol. 2005. Vol. 146. N 1. P. 98–108. Kerr P.M., Hillier K., Wallis R.M., Garland C.J. Characterization of muscarinic receptors mediating contractions of circular and longitudinal muscle of human isolated colon // Br. J. Pharmacol. 1995. Vol. 115. N 8. P. 1518–1524. Ehlert F.J. Contractile role of M2 and M3 muscarinic receptors in gastrointestinal, airway and urinary bladder smooth muscle // Life Sci. 2003. Vol. 74. N 2–3. P. 355–366. Hu X.Q., Zhang L. Functon and regulation of large conductance Ca2+ activated K+ channel in vascular smooth muscle cells // Drug Discov. Today. 2012. Vol. 17. N 17–18. P. 974–987. Tanahashi Y., Komori S., Matsuyama H., Kitazawa T., Unno T. Functions of muscarinic receptor subtypes in gastrointestinal smooth muscle: A review of studies with receptor-knockout mice // Int. J. Mol. Sci. 2021. Vol. 22. N 2. P. 1–24. Tran J.A., Matsui M., Ehlert F.J. Differential coupling of muscarinic M1, M2, and M3 receptors to phosphoinositide hydrolysis in urinary bladder and longitudinal muscle of the ileum of the mouse // J. Pharmacol. Exp. Ther. 2006. Vol. 318. N 2. P. 649–656. Glaza I. Szadujkis-Szadurski L., Szadujkis- Szadurski R., Gajdus M., Olkowska J. Modulating activity of M1 receptor to the reaction of ileal smooth muscle // Postepy Hig. Med. Dosw. 2011. Vol. 65. P. 478–481. Menozzi A., Pozzoli C., Poli E., Bontempi G., Serventi P., Meucci V., Intorre L., Bertini S. Role of muscarinic receptors in the contraction of jejunal smooth muscle in the horse: An in vitro study // Res. Vet. Sci. 2017. Vol. 115. P. 387–392. Karaki H., Weiss G.B. Calcium release in smooth muscle // Life Sci. 1988. Vol. 42. N 2. P. 111–122. Somlyo A.P., Somlyo A.V. Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase // Physiol. Rev. 2003. Vol. 83. N 4. P. 1325–1358. Sahin L., Cevik O.S., Koyuncu D.D., Buyukafsar K. Role of rho-kinase (ROCK) in tonic but not phasic contraction in the frog stomach smooth muscle // Life Sci. 2018. Vol. 198. P. 46–55. Loirand G., Cario-Toumaniantz C., Chardin P., Pacaud P. The Rho-related protein Rnd1 inhibits Ca2+ sensitization of rat smooth muscle // J. Physiol. 1999. Vol. 516. N 3. P. 825–834. Swärd K., Dreja K., Susnjar M., Hellstrand P., Hartshorne D.J. Walsh M.P. Inhibition of Rho-associated kinase blocks agonist-induced Ca2+ sensitization of myosin phosphorylation and force in guinea-pig ileum // J. Physiol. 2000. Vol. 522. N 1. P. 33–49. Bayguinov O., Dwyer L., Kim H., Marklew A., Sanders K. M., Koh S.D. Contribution of Rho-kinase to membrane excitability of murine colonic smooth muscle // Br. J. Pharmacol. 2011. Vol. 163. N 3. P. 638–648. Park S.Y., Song H.J., Sohn U.D. Participation of Rho-associated kinase in electrical stimulated and acetylcholine-induced contraction of feline esophageal smooth muscle // Eur. J. Pharmacol. 2009. Vol. 607. N 1–3. P. 220–225. Rattan S., Phillips B.R., Maxwell P.J. RhoA/Rho- Kinase: pathophysiologic and therapeutic implications in gastrointestinal smooth muscle tone and relaxation // Gastroenterology. 2010. Vol. 138. N 1. P. 13–18. Андрияшев А.П. Рыбы северных морей СССР. М.; Л.: Изд-во Акад. наук СССР, 1954. 567 с. Kitazawa T., Itoh K., Yaosaka N., Maruyama K., Matsuda K., Teraoka H., Kaiya H. Ghrelin does not affect gastrointestinal contractility in rainbow trout and goldfish in vitro // Gen. Comp. Endocrinol. 2012. Vol. 178. N 3. P. 539–545. Kitazawa T., Hoshi T., Chugun A. Effects of some autonomic drugs and neuropeptides on the mechanical activity of longitudinal and circular muscle strips isolated from the carp intestinal bulb (Cyprinus carpio) // Comp. Biochem. Physiol. Part – C: Toxicol. Pharmacol. 1990. Vol. 97. N 1. P. 13–24. Karila P., Holmgren S. Enteric reflexes and nitric oxide in the fish intestine // J. Exp. Biol. 1995. Vol. 198. N 11. P. 2405–2411. Harrington A.M., Peck C.J., Liu L., Burcher E., Hutson J. M., Southwell B.R. Localization of muscarinic receptors M1R, M2R and M3R in the human colon // Neurogastroenterol. Motil. 2010. Vol. 22. N 9: 999–e263. Honda K., Takano Y., Kamiya H. Pharmacological profiles of muscarinic receptors in the longitudinal smooth muscle of guinea pig ileum // Jpn. J. Pharmacol. 1993. Vol. 62. N 1. P. 43–47. Tobin G., Giglio D., Lundgren O. Muscarinic receptor subtypes in the alimentary tract // J. Physiol. Pharmacol. 2009. Vol. 60. N 1. P. 3–21. Hu J., Gao W.Y., Ma L., Man S.L., Huang L.Q., Liu C.X. Activation of M3 muscarinic receptor and Ca2+ influx by crude fraction from Crotonis Fructus in isolated rabbit jejunum // J. Ethnopharmacol. 2012. Vol. 139. N 1. P. 136–141. Harrington A.M., Hutson J.M., Southwell B.R. Cholinergic neurotransmission and muscarinic receptors in the enteric nervous system // Prog. Histochem. Cytochem. 2010. Vol. 44. N 4. P. 173–202. Uchiyama T., Chess-Williams R. Muscarinic receptor subtypes of the bladder and gastrointestinal tract // J. Smooth Muscle Res. 2004. Vol. 40. N 6. P. 237–247. Unno T., Matsuyama H., Izumi Y., Yamada M., Wess J., Komori S. Roles of M2 and M3 muscarinic receptors in cholinergic nerve-induced contractions in mouse ileum studied with receptor knockout mice // Br. J. Pharmacol. 2006. Vol. 149. N 8. P. 1022–1030. 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P. 1254–1262. https://vestnik-bio-msu.elpub.ru/jour/article/view/1184 Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access). Авторы, публикующие статьи в данном журнале, соглашаются на следующее:Авторы сохраняют за собой автороские права и предоставляют журналу право первой публикации работы, которая по истечении 6 месяцев после публикации автоматически лицензируется на условиях Creative Commons Attribution License , которая позволяет другим распространять данную работу с обязательным сохранением ссылок на авторов оригинальной работы и оригинальную публикацию в этом журнале.Авторы имеют право размещать их работу в сети Интернет (например, в институтском хранилище или на персональном сайте). CC-BY Vestnik Moskovskogo universiteta. Seriya 16. Biologiya; Том 77, № 4 (2022); 231-240 Вестник Московского университета. Серия 16. Биология; Том 77, № 4 (2022); 231-240 0137-0952 Rho-киназа smooth muscle intestine acetylcholine muscarinic receptors Rho-kinase гладкая мышца кишечник ацетилхолин мускари- новые холинорецепторы info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftjhmub 2022-12-06T01:11:57Z The study of the functioning of various body systems in different vertebrates is one of the key tasks of comparative physiology. Teleost fish are a large group of vertebrates, however, the mechanisms of functioning of their digestive tract have been studied little and mainly only for such a model object as Danio rerio. The aim of this work was to characterize the participation of various subtypes of M-cholinergic receptors and the enzyme Rho-kinase in the cholinergic contraction of the intestinal smooth muscles of the White Sea cod Gadus morhua marisalbi (Gadidae). A longitudinal strip was excised from the proximal cod intestine and placed in an apparatus for recording contractile responses in the isometric mode. Contractile responses to acetylcholine were completely blocked by atropine. Blockade of M3 cholinergic receptors with 4-DAMP resulted in a decrease in acetylcholine-induced contraction compared with the control response. Blockade of M1 receptors with pirenzepine led to a weakening of contraction, less pronounced than with blockade of M3 cholinergic receptors. Blockade of M2-cholinergic receptors with methoctramine did not affect the magnitude of the contractile response. Incubation of preparations with the Rho-kinase inhibitor fasudil was accompanied by a significant decrease in contractile responses compared with the control, as well as a faster decrease in the contraction force after reaching the “peak” of the reaction. Thus, acetylcholine causes contraction of cod intestinal smooth muscle by activating M3- and M1- but not M2-cholinergic receptors. The activity of the Rho-kinase enzyme contributes to the development and maintenance of cod intestinal smooth muscle contraction under the action of acetylcholine. The results obtained are of interest for comparative physiology, may be important for understanding the mechanisms of the damaging effect of environmental factors on the bony fish’ body, as well as for the use of fish as objects of preclinical studies of drugs. Изучение особенностей ... Article in Journal/Newspaper Gadus morhua White Sea Herald of Moscow University. Series 16. Biology White Sea Rho ENVELOPE(-63.000,-63.000,-64.300,-64.300)

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