Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity
Marine microalgae has been attracting the researcher’s attention for centuries. Development on microalgal research is majorly favoured by its medicinal, pharmaceutical or cosmeceutical properties. The advancement in the investigation related to microalgal products have been concentrated in the coast...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
2021
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Online Access: | https://gayanabotanica.cl/index.php/gb/article/view/351 |
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Gayana Botanica (E-Journal) |
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ftjgayanabotani |
language |
English |
topic |
Southern Ocean microalgae phenolic compound antibacterial activity microalga compuestos fenólicos actividad antibacteriana Océano Sur |
spellingShingle |
Southern Ocean microalgae phenolic compound antibacterial activity microalga compuestos fenólicos actividad antibacteriana Océano Sur Gupta, Pratibha Banerjee, Aparna Castillo, Alexis Bandopadhyay, Rajib Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity |
topic_facet |
Southern Ocean microalgae phenolic compound antibacterial activity microalga compuestos fenólicos actividad antibacteriana Océano Sur |
description |
Marine microalgae has been attracting the researcher’s attention for centuries. Development on microalgal research is majorly favoured by its medicinal, pharmaceutical or cosmeceutical properties. The advancement in the investigation related to microalgal products have been concentrated in the coastal zones because of the greater supply of raw material. The Southern Ocean, highly productive and relatively poorly studied ecosystem, constitutes approximately 10% of the global volume of the oceans. In this study, marine microalgae Chlorella sp. PR-1 was isolated from the Southern Ocean (Indian Sector) for the identification of bioactive antibacterial compounds. The antimicrobial activity of this microalgal extracts was evaluated against three gram positive (Staphylococcus aureus, Bacillus licheniformis, and Bacillus subtilis) and three gram negative bacteria (Pseudomonas aeruginosa, Salmonella typhimurium, and Escherichia coli). The extract showing antibacterial activity was further purified by thin layer and column chromatography. The antimicrobial activity was again evaluated and confirmed with the purified fraction against the same bacteria. The identification of the functional groups in the purified fraction was performed by infrared spectroscopic analysis. Based on gas chromatographic and mass spectroscopic analysis, the principle bioactive compound was proved to be 2,4-bis (1,1-dimethylethyl)- phenol. Thus, the bioactive compound isolated from marine microalga of Southern Ocean origin may be a novel alternative source of antibacterials in the future. Las microalgas marinas han atraído la atención de los investigadores durante siglos. El desarrollo de la investigación de las microalgas se ve favorecido principalmente debido a sus propiedades medicinales, farmacéuticas y cosméticas. El avance de las investigaciones relacionadas con productos microalgales se ha concentrado en las zonas costeras debido a la mayor oferta de materias primas. El Océano Sur es un ecosistema extremo altamente productivo y relativamente poco estudiado, constituye aproximadamente el 10% del volumen global de los océanos. En este estudio, la microalga marina Chlorella sp. PR-1 fue aislada desde el Océano Sur (sector Indico) para la identificación de compuestos bioactivos antibacterianos. La actividad antimicrobiana de los extractos microalgales fue evaluada contra tres especies bacterianas gran positivas (Staphylococcus aureus, Bacillus licheniformis y Bacillus subtilis) y tres gram negativas (Pseudomonas aeruginosa, Salmonella typhimurium y Escherichia coli). Los extractos que mostraron actividad antibacteriana fueron purificados mediante cromatografía de capa fina. La actividad antibacteriana de las fracciones purificadas fue evaluada nuevamente y confirmada contra las mismas bacterias. En las fracciones purificadas, la identificación de los grupos funcionales fue realizada mediante análisis espectroscópico infrarrojo. Basado en los análisis cromatográficos y de espectroscopia de masa se demostró que el principal compuesto bioactivo fue 2,4-bis (1,1-dimetiletil)-fenol. Por lo tanto, el compuesto bioactivo aislado de microalgas marinas del Océano Austral puede ser una nueva fuente alternativa de antibacterianos en el futuro. Palabras clave: actividad antibacteriana, compuestos fenólicos, microalga, Océano Sur. |
format |
Article in Journal/Newspaper |
author |
Gupta, Pratibha Banerjee, Aparna Castillo, Alexis Bandopadhyay, Rajib |
author_facet |
Gupta, Pratibha Banerjee, Aparna Castillo, Alexis Bandopadhyay, Rajib |
author_sort |
Gupta, Pratibha |
title |
Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity |
title_short |
Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity |
title_full |
Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity |
title_fullStr |
Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity |
title_full_unstemmed |
Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity |
title_sort |
novel phenolic compound from southern ocean microalgae chlorella sp. pr-1 and its antibacterial activity |
publisher |
Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción |
publishDate |
2021 |
url |
https://gayanabotanica.cl/index.php/gb/article/view/351 |
geographic |
Southern Ocean Austral Indian |
geographic_facet |
Southern Ocean Austral Indian |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Gayana Botanica; Vol. 78 No. 1 (2021); 29-37 Gayana Botanica; Vol. 78 Núm. 1 (2021); 29-37 0717-6643 0016-5301 |
op_relation |
https://gayanabotanica.cl/index.php/gb/article/view/351/156 https://gayanabotanica.cl/index.php/gb/article/view/351 |
op_rights |
Copyright (c) 2021 Pratibha Gupta, Aparna Banerjee, Alexis Castillo, Rajib Bandopadhyay https://creativecommons.org/licenses/by-nc/4.0 |
op_rightsnorm |
CC-BY-NC |
_version_ |
1766205924579475456 |
spelling |
ftjgayanabotani:oai:ojs2.ojs.gayanabotanica.cl:article/351 2023-05-15T18:24:54+02:00 Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity Nuevo compuesto fenólico antibacteriano aislado de la microalga Chlorella sp. PR-1 del océano sur Gupta, Pratibha Banerjee, Aparna Castillo, Alexis Bandopadhyay, Rajib 2021-06-30 application/pdf https://gayanabotanica.cl/index.php/gb/article/view/351 eng eng Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción https://gayanabotanica.cl/index.php/gb/article/view/351/156 https://gayanabotanica.cl/index.php/gb/article/view/351 Copyright (c) 2021 Pratibha Gupta, Aparna Banerjee, Alexis Castillo, Rajib Bandopadhyay https://creativecommons.org/licenses/by-nc/4.0 CC-BY-NC Gayana Botanica; Vol. 78 No. 1 (2021); 29-37 Gayana Botanica; Vol. 78 Núm. 1 (2021); 29-37 0717-6643 0016-5301 Southern Ocean microalgae phenolic compound antibacterial activity microalga compuestos fenólicos actividad antibacteriana Océano Sur info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftjgayanabotani 2022-02-22T13:03:06Z Marine microalgae has been attracting the researcher’s attention for centuries. Development on microalgal research is majorly favoured by its medicinal, pharmaceutical or cosmeceutical properties. The advancement in the investigation related to microalgal products have been concentrated in the coastal zones because of the greater supply of raw material. The Southern Ocean, highly productive and relatively poorly studied ecosystem, constitutes approximately 10% of the global volume of the oceans. In this study, marine microalgae Chlorella sp. PR-1 was isolated from the Southern Ocean (Indian Sector) for the identification of bioactive antibacterial compounds. The antimicrobial activity of this microalgal extracts was evaluated against three gram positive (Staphylococcus aureus, Bacillus licheniformis, and Bacillus subtilis) and three gram negative bacteria (Pseudomonas aeruginosa, Salmonella typhimurium, and Escherichia coli). The extract showing antibacterial activity was further purified by thin layer and column chromatography. The antimicrobial activity was again evaluated and confirmed with the purified fraction against the same bacteria. The identification of the functional groups in the purified fraction was performed by infrared spectroscopic analysis. Based on gas chromatographic and mass spectroscopic analysis, the principle bioactive compound was proved to be 2,4-bis (1,1-dimethylethyl)- phenol. Thus, the bioactive compound isolated from marine microalga of Southern Ocean origin may be a novel alternative source of antibacterials in the future. Las microalgas marinas han atraído la atención de los investigadores durante siglos. El desarrollo de la investigación de las microalgas se ve favorecido principalmente debido a sus propiedades medicinales, farmacéuticas y cosméticas. El avance de las investigaciones relacionadas con productos microalgales se ha concentrado en las zonas costeras debido a la mayor oferta de materias primas. El Océano Sur es un ecosistema extremo altamente productivo y relativamente poco estudiado, constituye aproximadamente el 10% del volumen global de los océanos. En este estudio, la microalga marina Chlorella sp. PR-1 fue aislada desde el Océano Sur (sector Indico) para la identificación de compuestos bioactivos antibacterianos. La actividad antimicrobiana de los extractos microalgales fue evaluada contra tres especies bacterianas gran positivas (Staphylococcus aureus, Bacillus licheniformis y Bacillus subtilis) y tres gram negativas (Pseudomonas aeruginosa, Salmonella typhimurium y Escherichia coli). Los extractos que mostraron actividad antibacteriana fueron purificados mediante cromatografía de capa fina. La actividad antibacteriana de las fracciones purificadas fue evaluada nuevamente y confirmada contra las mismas bacterias. En las fracciones purificadas, la identificación de los grupos funcionales fue realizada mediante análisis espectroscópico infrarrojo. Basado en los análisis cromatográficos y de espectroscopia de masa se demostró que el principal compuesto bioactivo fue 2,4-bis (1,1-dimetiletil)-fenol. Por lo tanto, el compuesto bioactivo aislado de microalgas marinas del Océano Austral puede ser una nueva fuente alternativa de antibacterianos en el futuro. Palabras clave: actividad antibacteriana, compuestos fenólicos, microalga, Océano Sur. Article in Journal/Newspaper Southern Ocean Gayana Botanica (E-Journal) Southern Ocean Austral Indian |