Validation of suitable reference genes for quantitative real-time PCR normalization in Crassostrea gigas spat stage during toxic dinoflagellates exposure//Validación de genes de referencia adecuados para la normalización de PCR cuantitativa en tiempo real de juveniles de Crassostrea gigas expuestos a dinoflagelados tóxicos

• Published in: Biotecnia
• Main Authors: Romero Geraldo, Reyna, Hernández Saavedra, Norma, Fimbres Olivarría, Diana, García Lagunas, Norma
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidad de Sonora 2020
• Subjects: Gene expression; reference gene; normalization; toxic dinoflagellates; Crassostrea gigas; Cadena; Referencia
• Online Access: https://biotecnia.unison.mx/index.php/biotecnia/article/view/1250; https://doi.org/10.18633/biotecnia.v22i2.1250

The quantitative real-time polymerase chain reaction is a widely used method for gene expression analysis requiring carefully selected reference genes to ensure data validity. Regardless of several studies on gene expression bivalves and particularly Crassostrea gigas, has not been fully investigate...