Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue
Interleukin-1 Receptor 8 (IL-1R8) is a transmembrane protein of the IL-1 receptor family and represents an important regulator of the balance of innate and inflammatory responses. Depending on the immunological insult, IL1-R8 protects from the immunopathology or impairs the protective immune respons...
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ftjavr:oai:ojs.pkp.sfu.ca:article/1087 2023-05-15T15:51:01+02:00 Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue Turin, Lauretta Bianchessi, Laura Filipe, Joel Inglesi, Alessia Riva, Federica 2022-10-04 application/pdf https://www.advetresearch.com/index.php/AVR/article/view/1087 eng eng Journal of Advanced Veterinary Research https://www.advetresearch.com/index.php/AVR/article/view/1087/594 https://www.advetresearch.com/index.php/AVR/article/view/1087 Copyright (c) 2022 Journal of Advanced Veterinary Research https://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND Journal of Advanced Veterinary Research; Vol. 12 No. 5 (2022): October; 613-617 2090-6277 2090-6269 canine dog IL-1R8 innate immunity receptor info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2022 ftjavr 2022-10-11T17:25:13Z Interleukin-1 Receptor 8 (IL-1R8) is a transmembrane protein of the IL-1 receptor family and represents an important regulator of the balance of innate and inflammatory responses. Depending on the immunological insult, IL1-R8 protects from the immunopathology or impairs the protective immune response against the insult. The expression pattern of IL-1R8 in dog tissues is unknown. Given the relevance of inflammatory diseases in dog, the aims of this study were to identify the sequence, analyze the phylogenesis and investigate the differential expression and distribution of IL-1R8 in a wide panel of non-pathologic tissues and organs by means of quantitative Real-Time PCR and uncover species-specific peculiarities. In Canis lupus familiaris, the IL1-R8 gene maps on chromosome 18, and includes ten exons. We first compared the coding sequence of dog IL-1R8 with sequences of other carnivors. Phylogenetic analysis revealed that IL-1R8 shares significantly high sequence homology with IL-1R8 of other canids particularly fox, sharing a common progenitor. Our study demonstrated that IL-1R8 is highly expressed in pancreas, considerably expressed in kidney, heart, liver, skeletal muscle, thymus, salivary gland, lymph node and lung. Interestingly, the expression pattern disclosed a unique profile for canine tissues when compared to tissues from other animal’s species. Imbalance of pro-inflammatory response leads to a vicious loop whither pro-inflammatory signaling and injury sustain each other and booster the disease. Therefore, it is crucial to investigate key regulator molecules such as IL-1R8, which functions both in homeostasis and disease and has potential to be a valid diagnostic, prognostic and therapeutic biomarker. Article in Journal/Newspaper Canis lupus Journal of Advanced Veterinary Research (University Assiut, Egypt) |
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Open Polar |
collection |
Journal of Advanced Veterinary Research (University Assiut, Egypt) |
op_collection_id |
ftjavr |
language |
English |
topic |
canine dog IL-1R8 innate immunity receptor |
spellingShingle |
canine dog IL-1R8 innate immunity receptor Turin, Lauretta Bianchessi, Laura Filipe, Joel Inglesi, Alessia Riva, Federica Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue |
topic_facet |
canine dog IL-1R8 innate immunity receptor |
description |
Interleukin-1 Receptor 8 (IL-1R8) is a transmembrane protein of the IL-1 receptor family and represents an important regulator of the balance of innate and inflammatory responses. Depending on the immunological insult, IL1-R8 protects from the immunopathology or impairs the protective immune response against the insult. The expression pattern of IL-1R8 in dog tissues is unknown. Given the relevance of inflammatory diseases in dog, the aims of this study were to identify the sequence, analyze the phylogenesis and investigate the differential expression and distribution of IL-1R8 in a wide panel of non-pathologic tissues and organs by means of quantitative Real-Time PCR and uncover species-specific peculiarities. In Canis lupus familiaris, the IL1-R8 gene maps on chromosome 18, and includes ten exons. We first compared the coding sequence of dog IL-1R8 with sequences of other carnivors. Phylogenetic analysis revealed that IL-1R8 shares significantly high sequence homology with IL-1R8 of other canids particularly fox, sharing a common progenitor. Our study demonstrated that IL-1R8 is highly expressed in pancreas, considerably expressed in kidney, heart, liver, skeletal muscle, thymus, salivary gland, lymph node and lung. Interestingly, the expression pattern disclosed a unique profile for canine tissues when compared to tissues from other animal’s species. Imbalance of pro-inflammatory response leads to a vicious loop whither pro-inflammatory signaling and injury sustain each other and booster the disease. Therefore, it is crucial to investigate key regulator molecules such as IL-1R8, which functions both in homeostasis and disease and has potential to be a valid diagnostic, prognostic and therapeutic biomarker. |
format |
Article in Journal/Newspaper |
author |
Turin, Lauretta Bianchessi, Laura Filipe, Joel Inglesi, Alessia Riva, Federica |
author_facet |
Turin, Lauretta Bianchessi, Laura Filipe, Joel Inglesi, Alessia Riva, Federica |
author_sort |
Turin, Lauretta |
title |
Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue |
title_short |
Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue |
title_full |
Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue |
title_fullStr |
Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue |
title_full_unstemmed |
Phylogeny and Differential Gene Expression Profile of IL-1R8 in Normal Dog Tissue |
title_sort |
phylogeny and differential gene expression profile of il-1r8 in normal dog tissue |
publisher |
Journal of Advanced Veterinary Research |
publishDate |
2022 |
url |
https://www.advetresearch.com/index.php/AVR/article/view/1087 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Journal of Advanced Veterinary Research; Vol. 12 No. 5 (2022): October; 613-617 2090-6277 2090-6269 |
op_relation |
https://www.advetresearch.com/index.php/AVR/article/view/1087/594 https://www.advetresearch.com/index.php/AVR/article/view/1087 |
op_rights |
Copyright (c) 2022 Journal of Advanced Veterinary Research https://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766386063793717248 |