Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization
Chromolaena odorata is a plant that has been used traditionally in the treatment of diverse disease conditions. This study sort to evaluate the anti-Salmonella typhi potential of Chromolaena odorata compared to ciprofloxacin in Wistar rats and to identify the phytochemical constituents present in th...
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ftjasrjets:oai:ojs.asrjetsjournal.org:article/4030 2023-05-15T15:52:59+02:00 Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization Charles, Joshua Isirima Minakiri, Iyeopu Siminialayi 2018-04-19 application/pdf http://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4030 eng eng American Scientific Research Journal for Engineering, Technology, and Sciences http://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4030/1442 http://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4030 Copyright (c) 2019 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS); Vol 42 No 1 (2018); 111-129 2313-4402 2313-4410 Chromolaena odorata Phytochemical Screening GC-MS analysis Salmonella typhi Wistar Rats info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2018 ftjasrjets 2021-06-20T09:23:11Z Chromolaena odorata is a plant that has been used traditionally in the treatment of diverse disease conditions. This study sort to evaluate the anti-Salmonella typhi potential of Chromolaena odorata compared to ciprofloxacin in Wistar rats and to identify the phytochemical constituents present in the plant that could be responsible for the antibacterial activity exhibited by the plant. The animals were divided into six groups: group 1 was normal control, group 2 was infected with Salmonella typhi without treatment, groups 3, 4 and 5 were Salmonella typhi infected and treated with 100mg/kg, 200mg/kg and 400mg/kg of the extract respectively and group 6 was infected and treated with 500mg/70kg of ciprofloxacin. The animals were inoculated with a single infective dose of Salmonella typhi bacteria and subsequently, treated with the graded doses of methanol leaf extract of Chromolaena odorata and 500mg/70kg dose of ciprofloxacin for a period of fifteen days, when the animals were confirmed infected. Fecal matter was collected from each group on day 0 (when the animals were confirmed infected) and on every five days interval for the period of the study, for Salmonella typhi load assay. This was followed by phytochemical screening and GC-MS analysis of the plant extract. Treatment of infected animals with the extract and standard drug, resulted in a dose and time dependent eradication of the Salmonella typhi from the stool, while the phytochemical screening and the GC-MS analysis revealed the presence of phenolic, flavonoid, alkaloid, steroid and Geijerene, 3-methyl-3-vinyl- cyclopropene; 4, 6-Dimethyl-3-nitro-2(1H)-pyridinone, 2-Hexyn-1-ol, 1,2,3,4-Tetrahydro-2,3-dimethylquinoxaline, nonyl prop-1-en-2-yl ester Carbonic acid respectively. Thus the observed antibacterial activity could be attributed to the presence of these phytochemicals. Article in Journal/Newspaper Carbonic acid American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) |
institution |
Open Polar |
collection |
American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) |
op_collection_id |
ftjasrjets |
language |
English |
topic |
Chromolaena odorata Phytochemical Screening GC-MS analysis Salmonella typhi Wistar Rats |
spellingShingle |
Chromolaena odorata Phytochemical Screening GC-MS analysis Salmonella typhi Wistar Rats Charles, Joshua Isirima Minakiri, Iyeopu Siminialayi Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization |
topic_facet |
Chromolaena odorata Phytochemical Screening GC-MS analysis Salmonella typhi Wistar Rats |
description |
Chromolaena odorata is a plant that has been used traditionally in the treatment of diverse disease conditions. This study sort to evaluate the anti-Salmonella typhi potential of Chromolaena odorata compared to ciprofloxacin in Wistar rats and to identify the phytochemical constituents present in the plant that could be responsible for the antibacterial activity exhibited by the plant. The animals were divided into six groups: group 1 was normal control, group 2 was infected with Salmonella typhi without treatment, groups 3, 4 and 5 were Salmonella typhi infected and treated with 100mg/kg, 200mg/kg and 400mg/kg of the extract respectively and group 6 was infected and treated with 500mg/70kg of ciprofloxacin. The animals were inoculated with a single infective dose of Salmonella typhi bacteria and subsequently, treated with the graded doses of methanol leaf extract of Chromolaena odorata and 500mg/70kg dose of ciprofloxacin for a period of fifteen days, when the animals were confirmed infected. Fecal matter was collected from each group on day 0 (when the animals were confirmed infected) and on every five days interval for the period of the study, for Salmonella typhi load assay. This was followed by phytochemical screening and GC-MS analysis of the plant extract. Treatment of infected animals with the extract and standard drug, resulted in a dose and time dependent eradication of the Salmonella typhi from the stool, while the phytochemical screening and the GC-MS analysis revealed the presence of phenolic, flavonoid, alkaloid, steroid and Geijerene, 3-methyl-3-vinyl- cyclopropene; 4, 6-Dimethyl-3-nitro-2(1H)-pyridinone, 2-Hexyn-1-ol, 1,2,3,4-Tetrahydro-2,3-dimethylquinoxaline, nonyl prop-1-en-2-yl ester Carbonic acid respectively. Thus the observed antibacterial activity could be attributed to the presence of these phytochemicals. |
format |
Article in Journal/Newspaper |
author |
Charles, Joshua Isirima Minakiri, Iyeopu Siminialayi |
author_facet |
Charles, Joshua Isirima Minakiri, Iyeopu Siminialayi |
author_sort |
Charles, Joshua Isirima |
title |
Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization |
title_short |
Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization |
title_full |
Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization |
title_fullStr |
Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization |
title_full_unstemmed |
Evaluation of the Antibacterial Activity of Chromolaena Odorata in Wistar Rats and its Chemical Characterization |
title_sort |
evaluation of the antibacterial activity of chromolaena odorata in wistar rats and its chemical characterization |
publisher |
American Scientific Research Journal for Engineering, Technology, and Sciences |
publishDate |
2018 |
url |
http://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4030 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS); Vol 42 No 1 (2018); 111-129 2313-4402 2313-4410 |
op_relation |
http://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4030/1442 http://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/4030 |
op_rights |
Copyright (c) 2019 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) |
_version_ |
1766388061878353920 |