Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads
A double immobilisation technique was developed for C. antarctica lipase (CALB) that improved its enzymatic activity and solved difficulties frequently observed in reactions catalysed by lipases. The first immobilisation consisted of CALB adsorption onto a TiO2 nanoparticle surface (CALB TiO2). The...
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ftjamescook:oai:researchonline.jcu.edu.au:73869 2024-02-11T09:58:03+01:00 Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads Welter, Rosilene Santana, Harrson Silva Carvalho, Bruna Gregatti Melani, Natalia Oelgemoeller, Michael de la Torre, Lucimara Gaziola Taranto, Osvaldir Pereira 2022 application/pdf https://researchonline.jcu.edu.au/73869/7/73869.pdf unknown Elsevier https://doi.org/10.1016/j.jiec.2022.03.028 https://researchonline.jcu.edu.au/73869/ https://researchonline.jcu.edu.au/73869/7/73869.pdf Welter, Rosilene, Santana, Harrson Silva, Carvalho, Bruna Gregatti, Melani, Natalia, Oelgemoeller, Michael, de la Torre, Lucimara Gaziola, and Taranto, Osvaldir Pereira (2022) Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads. Journal of Industrial and Engineering Chemistry, 110. pp. 576-586. restricted Article PeerReviewed 2022 ftjamescook https://doi.org/10.1016/j.jiec.2022.03.028 2024-01-22T23:50:55Z A double immobilisation technique was developed for C. antarctica lipase (CALB) that improved its enzymatic activity and solved difficulties frequently observed in reactions catalysed by lipases. The first immobilisation consisted of CALB adsorption onto a TiO2 nanoparticle surface (CALB TiO2). The adsorption was carried out by an oriented monolayer formed by CALB’s hydrophilic amino acids and the TiO2 surface, leaving the CALB’s active site accessible for reaction. As a result, an increase in enzymatic activity was achieved. The Relative Enzymatic Activity ((REA) obtained was 289%. The second immobilisation consisted of CALBTiO2 entrapment into calcium alginate microbeads [(CALBTiO2)EDTA-Ca], obtained by an internal crosslinking ion-exchange mechanism and using microfluidic droplet technique. The microbiocatalyst obtained, (CALBTiO2)EDTA-Ca, retained a high enzymatic activity (REAt=0 = 232%) and stability (REAt=30days = 263%) at a size (diameter, Ø=8.9·104 nm) that enabled easier recovery than CALBTiO2 (Ø = 2.3·102 nm) or CALBFree (Ø=5.0 nm), and showed a favourable porosity for diffusion without releasing CALBTiO2. Although the microbeads showed CALBFree leaching, as demonstrated by the loss of REA after a mechanical resistant test, (CALBTiO2)EDTA-Ca maintained an almost constant REA. Article in Journal/Newspaper Antarc* Antarctica James Cook University, Australia: ResearchOnline@JCU Journal of Industrial and Engineering Chemistry 110 576 586 |
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Open Polar |
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James Cook University, Australia: ResearchOnline@JCU |
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ftjamescook |
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description |
A double immobilisation technique was developed for C. antarctica lipase (CALB) that improved its enzymatic activity and solved difficulties frequently observed in reactions catalysed by lipases. The first immobilisation consisted of CALB adsorption onto a TiO2 nanoparticle surface (CALB TiO2). The adsorption was carried out by an oriented monolayer formed by CALB’s hydrophilic amino acids and the TiO2 surface, leaving the CALB’s active site accessible for reaction. As a result, an increase in enzymatic activity was achieved. The Relative Enzymatic Activity ((REA) obtained was 289%. The second immobilisation consisted of CALBTiO2 entrapment into calcium alginate microbeads [(CALBTiO2)EDTA-Ca], obtained by an internal crosslinking ion-exchange mechanism and using microfluidic droplet technique. The microbiocatalyst obtained, (CALBTiO2)EDTA-Ca, retained a high enzymatic activity (REAt=0 = 232%) and stability (REAt=30days = 263%) at a size (diameter, Ø=8.9·104 nm) that enabled easier recovery than CALBTiO2 (Ø = 2.3·102 nm) or CALBFree (Ø=5.0 nm), and showed a favourable porosity for diffusion without releasing CALBTiO2. Although the microbeads showed CALBFree leaching, as demonstrated by the loss of REA after a mechanical resistant test, (CALBTiO2)EDTA-Ca maintained an almost constant REA. |
format |
Article in Journal/Newspaper |
author |
Welter, Rosilene Santana, Harrson Silva Carvalho, Bruna Gregatti Melani, Natalia Oelgemoeller, Michael de la Torre, Lucimara Gaziola Taranto, Osvaldir Pereira |
spellingShingle |
Welter, Rosilene Santana, Harrson Silva Carvalho, Bruna Gregatti Melani, Natalia Oelgemoeller, Michael de la Torre, Lucimara Gaziola Taranto, Osvaldir Pereira Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads |
author_facet |
Welter, Rosilene Santana, Harrson Silva Carvalho, Bruna Gregatti Melani, Natalia Oelgemoeller, Michael de la Torre, Lucimara Gaziola Taranto, Osvaldir Pereira |
author_sort |
Welter, Rosilene |
title |
Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads |
title_short |
Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads |
title_full |
Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads |
title_fullStr |
Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads |
title_full_unstemmed |
Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads |
title_sort |
droplet microfluidics for double lipase immobilisation using tio2 and alginate microbeads |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://researchonline.jcu.edu.au/73869/7/73869.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://doi.org/10.1016/j.jiec.2022.03.028 https://researchonline.jcu.edu.au/73869/ https://researchonline.jcu.edu.au/73869/7/73869.pdf Welter, Rosilene, Santana, Harrson Silva, Carvalho, Bruna Gregatti, Melani, Natalia, Oelgemoeller, Michael, de la Torre, Lucimara Gaziola, and Taranto, Osvaldir Pereira (2022) Droplet microfluidics for double lipase immobilisation using TiO2 and alginate microbeads. Journal of Industrial and Engineering Chemistry, 110. pp. 576-586. |
op_rights |
restricted |
op_doi |
https://doi.org/10.1016/j.jiec.2022.03.028 |
container_title |
Journal of Industrial and Engineering Chemistry |
container_volume |
110 |
container_start_page |
576 |
op_container_end_page |
586 |
_version_ |
1790593622198976512 |