Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish

• Published in: Conservation Physiology
• Main Authors: Andrewartha, Sarah J., Munns, Suzanne L., Edwards, Ashley
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Oxford University Press 2016
• Subjects: Atlantic salmon; Salmo salar
• Online Access: https://researchonline.jcu.edu.au/43423/1/Conserv%20Physiol-2016-Andrewartha-.pdf

Haemoglobin concentration ([Hb]) is measured for a wide variety of animal studies. The use of point-of-care devices, such as the HemoCue, is becoming increasingly common because of their portability, relative ease of use and low cost. In this study, we aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be determined accurately using the HemoCue and whether the HemoCue overestimates the [Hb] of reptile blood in a similar manner to fish blood. Additionally, we aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201⁺ systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon, and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species and, as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.