Species identification in the trematomid family using nuclear genetic markers

The Trematominae, a subfamily of the Nototheniidae, are typical of the high-Antarctic shelf waters. Within the Trematominae examples of phenotypic plasticity and possible cryptic speciation have been observed. Morphological identification of adult stages can be problematic in cases of high phenotypi...

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Published in:Polar Biology
Main Authors: Van de Putte, Anton Pieter, Van Houdt, J.K.J., Maes, G.E., Janko, K., Koubbi, P., Rock, J., Volckaert, F.A.M.
Format: Article in Journal/Newspaper
Language:unknown
Published: Springer 2009
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Online Access:https://researchonline.jcu.edu.au/30026/1/30026_Van_de_Putte_et_al_2009.pdf
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spelling ftjamescook:oai:researchonline.jcu.edu.au:30026 2024-02-11T09:58:42+01:00 Species identification in the trematomid family using nuclear genetic markers Van de Putte, Anton Pieter Van Houdt, J.K.J. Maes, G.E. Janko, K. Koubbi, P. Rock, J. Volckaert, F.A.M. 2009 application/pdf https://researchonline.jcu.edu.au/30026/1/30026_Van_de_Putte_et_al_2009.pdf unknown Springer http://dx.doi.org/10.1007/s00300-009-0672-8 https://researchonline.jcu.edu.au/30026/ https://researchonline.jcu.edu.au/30026/1/30026_Van_de_Putte_et_al_2009.pdf Van de Putte, Anton Pieter, Van Houdt, J.K.J., Maes, G.E., Janko, K., Koubbi, P., Rock, J., and Volckaert, F.A.M. (2009) Species identification in the trematomid family using nuclear genetic markers. Polar Biology, 32 (12). pp. 1731-1741. restricted Article PeerReviewed 2009 ftjamescook https://doi.org/10.1007/s00300-009-0672-8 2024-01-22T23:31:51Z The Trematominae, a subfamily of the Nototheniidae, are typical of the high-Antarctic shelf waters. Within the Trematominae examples of phenotypic plasticity and possible cryptic speciation have been observed. Morphological identification of adult stages can be problematic in cases of high phenotypic plasticity or cryptic speciation. Additionally, postlarval and juvenile stages often have traits still under development and which lack distinction. A microsatellite DNA multiplex of six markers has been developed for Trematomus newnesi (Van Houdt et al. 2006). This multiplex was tested on five additional trematomid taxa: Pagothenia borchgrevinki, Trematomus bernacchii, Trematomus eulepidotus, Trematomus hansoni and Trematomus scotti. We used these six microsatellite loci to assess the genetic differentiation among species and the resolution power of these loci for individual-based assignment methods. The six species could be well discriminated by conventional methods such as principal component analysis and distance-based methods, and individual Bayesian assignment methods. This marker set can be used for a number of purposes, including the identification of eggs and larval and adult stages. It is also useful for the investigation of recent phylogenetic patterns, as well as the detection of cryptic speciation, which has been suggested for T. bernacchii and T. newnesi but never confirmed with high polymorphic genetic markers. Article in Journal/Newspaper Antarc* Antarctic Polar Biology James Cook University, Australia: ResearchOnline@JCU Antarctic Polar Biology 32 12 1731 1741
institution Open Polar
collection James Cook University, Australia: ResearchOnline@JCU
op_collection_id ftjamescook
language unknown
description The Trematominae, a subfamily of the Nototheniidae, are typical of the high-Antarctic shelf waters. Within the Trematominae examples of phenotypic plasticity and possible cryptic speciation have been observed. Morphological identification of adult stages can be problematic in cases of high phenotypic plasticity or cryptic speciation. Additionally, postlarval and juvenile stages often have traits still under development and which lack distinction. A microsatellite DNA multiplex of six markers has been developed for Trematomus newnesi (Van Houdt et al. 2006). This multiplex was tested on five additional trematomid taxa: Pagothenia borchgrevinki, Trematomus bernacchii, Trematomus eulepidotus, Trematomus hansoni and Trematomus scotti. We used these six microsatellite loci to assess the genetic differentiation among species and the resolution power of these loci for individual-based assignment methods. The six species could be well discriminated by conventional methods such as principal component analysis and distance-based methods, and individual Bayesian assignment methods. This marker set can be used for a number of purposes, including the identification of eggs and larval and adult stages. It is also useful for the investigation of recent phylogenetic patterns, as well as the detection of cryptic speciation, which has been suggested for T. bernacchii and T. newnesi but never confirmed with high polymorphic genetic markers.
format Article in Journal/Newspaper
author Van de Putte, Anton Pieter
Van Houdt, J.K.J.
Maes, G.E.
Janko, K.
Koubbi, P.
Rock, J.
Volckaert, F.A.M.
spellingShingle Van de Putte, Anton Pieter
Van Houdt, J.K.J.
Maes, G.E.
Janko, K.
Koubbi, P.
Rock, J.
Volckaert, F.A.M.
Species identification in the trematomid family using nuclear genetic markers
author_facet Van de Putte, Anton Pieter
Van Houdt, J.K.J.
Maes, G.E.
Janko, K.
Koubbi, P.
Rock, J.
Volckaert, F.A.M.
author_sort Van de Putte, Anton Pieter
title Species identification in the trematomid family using nuclear genetic markers
title_short Species identification in the trematomid family using nuclear genetic markers
title_full Species identification in the trematomid family using nuclear genetic markers
title_fullStr Species identification in the trematomid family using nuclear genetic markers
title_full_unstemmed Species identification in the trematomid family using nuclear genetic markers
title_sort species identification in the trematomid family using nuclear genetic markers
publisher Springer
publishDate 2009
url https://researchonline.jcu.edu.au/30026/1/30026_Van_de_Putte_et_al_2009.pdf
geographic Antarctic
geographic_facet Antarctic
genre Antarc*
Antarctic
Polar Biology
genre_facet Antarc*
Antarctic
Polar Biology
op_relation http://dx.doi.org/10.1007/s00300-009-0672-8
https://researchonline.jcu.edu.au/30026/
https://researchonline.jcu.edu.au/30026/1/30026_Van_de_Putte_et_al_2009.pdf
Van de Putte, Anton Pieter, Van Houdt, J.K.J., Maes, G.E., Janko, K., Koubbi, P., Rock, J., and Volckaert, F.A.M. (2009) Species identification in the trematomid family using nuclear genetic markers. Polar Biology, 32 (12). pp. 1731-1741.
op_rights restricted
op_doi https://doi.org/10.1007/s00300-009-0672-8
container_title Polar Biology
container_volume 32
container_issue 12
container_start_page 1731
op_container_end_page 1741
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