The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera
This study explored the interactive effects of near-term CO₂ increases (40–90 ppm above current ambient) during a simulated bleaching event (34°C for 5d) of Acropora aspera by linking physiology to expression patterns of genes involved in carbon metabolism. Symbiodinium photosynthetic efficiency (F(...
Published in: | Coral Reefs |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Springer
2013
|
Subjects: | |
Online Access: | https://researchonline.jcu.edu.au/27887/1/27887_Ogawa_et_al_2013.pdf |
id |
ftjamescook:oai:researchonline.jcu.edu.au:27887 |
---|---|
record_format |
openpolar |
spelling |
ftjamescook:oai:researchonline.jcu.edu.au:27887 2024-02-11T10:07:34+01:00 The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera Ogawa, D. Bobeszko, T. Ainsworth, T. Leggat, W. 2013-12 application/pdf https://researchonline.jcu.edu.au/27887/1/27887_Ogawa_et_al_2013.pdf unknown Springer http://dx.doi.org/10.1007/s00338-013-1046-9 https://researchonline.jcu.edu.au/27887/ https://researchonline.jcu.edu.au/27887/1/27887_Ogawa_et_al_2013.pdf Ogawa, D., Bobeszko, T., Ainsworth, T., and Leggat, W. (2013) The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera. Coral Reefs, 32 (4). pp. 895-907. restricted Article PeerReviewed 2013 ftjamescook https://doi.org/10.1007/s00338-013-1046-9 2024-01-22T23:31:02Z This study explored the interactive effects of near-term CO₂ increases (40–90 ppm above current ambient) during a simulated bleaching event (34°C for 5d) of Acropora aspera by linking physiology to expression patterns of genes involved in carbon metabolism. Symbiodinium photosynthetic efficiency (F(v)/F(m)) was significantly depressed by the bleaching event, while elevated pressure of CO₂ (pCO₂) slightly mitigated the effects of increased temperature on F(v)/F(m) during the final 4 d of the recovery period, however, did not affect the loss of symbionts. Elevated pCO₂ alone had no effect on F(v)/F(m) or symbiont density. Expression of targeted Symbiodinium genes involved in carbon metabolism and heat stress response was not significantly altered by either increased temperature and/or CO₂. Of the selected host genes, two carbonic anhydrase isoforms (coCA2 and coCA3) exhibited the largest changes, most notably in crossed bleaching and elevated pCO₂ treatments. CA2 was significantly down-regulated on day 14 in all treatments, with the greatest decrease in the crossed treatment (relative expression compared to control = 0.16; p < 0.05); CA3 showed a similar trend, with expression levels 0.20-fold of controls on day 14 (p<0.05) in the elevated temperature/pCO₂ treatment. The synergistic effects of ocean acidification and bleaching were evident during this study and demonstrate that increased pCO₂ in surface waters will impact corals much sooner than many studies utilising end-of-century pCO₂ concentrations would indicate. Article in Journal/Newspaper Ocean acidification James Cook University, Australia: ResearchOnline@JCU Coral Reefs 32 4 895 907 |
institution |
Open Polar |
collection |
James Cook University, Australia: ResearchOnline@JCU |
op_collection_id |
ftjamescook |
language |
unknown |
description |
This study explored the interactive effects of near-term CO₂ increases (40–90 ppm above current ambient) during a simulated bleaching event (34°C for 5d) of Acropora aspera by linking physiology to expression patterns of genes involved in carbon metabolism. Symbiodinium photosynthetic efficiency (F(v)/F(m)) was significantly depressed by the bleaching event, while elevated pressure of CO₂ (pCO₂) slightly mitigated the effects of increased temperature on F(v)/F(m) during the final 4 d of the recovery period, however, did not affect the loss of symbionts. Elevated pCO₂ alone had no effect on F(v)/F(m) or symbiont density. Expression of targeted Symbiodinium genes involved in carbon metabolism and heat stress response was not significantly altered by either increased temperature and/or CO₂. Of the selected host genes, two carbonic anhydrase isoforms (coCA2 and coCA3) exhibited the largest changes, most notably in crossed bleaching and elevated pCO₂ treatments. CA2 was significantly down-regulated on day 14 in all treatments, with the greatest decrease in the crossed treatment (relative expression compared to control = 0.16; p < 0.05); CA3 showed a similar trend, with expression levels 0.20-fold of controls on day 14 (p<0.05) in the elevated temperature/pCO₂ treatment. The synergistic effects of ocean acidification and bleaching were evident during this study and demonstrate that increased pCO₂ in surface waters will impact corals much sooner than many studies utilising end-of-century pCO₂ concentrations would indicate. |
format |
Article in Journal/Newspaper |
author |
Ogawa, D. Bobeszko, T. Ainsworth, T. Leggat, W. |
spellingShingle |
Ogawa, D. Bobeszko, T. Ainsworth, T. Leggat, W. The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera |
author_facet |
Ogawa, D. Bobeszko, T. Ainsworth, T. Leggat, W. |
author_sort |
Ogawa, D. |
title |
The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera |
title_short |
The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera |
title_full |
The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera |
title_fullStr |
The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera |
title_full_unstemmed |
The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera |
title_sort |
combined effects of temperature and co₂ lead to altered gene expression in acropora aspera |
publisher |
Springer |
publishDate |
2013 |
url |
https://researchonline.jcu.edu.au/27887/1/27887_Ogawa_et_al_2013.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
http://dx.doi.org/10.1007/s00338-013-1046-9 https://researchonline.jcu.edu.au/27887/ https://researchonline.jcu.edu.au/27887/1/27887_Ogawa_et_al_2013.pdf Ogawa, D., Bobeszko, T., Ainsworth, T., and Leggat, W. (2013) The combined effects of temperature and CO₂ lead to altered gene expression in Acropora aspera. Coral Reefs, 32 (4). pp. 895-907. |
op_rights |
restricted |
op_doi |
https://doi.org/10.1007/s00338-013-1046-9 |
container_title |
Coral Reefs |
container_volume |
32 |
container_issue |
4 |
container_start_page |
895 |
op_container_end_page |
907 |
_version_ |
1790606181009457152 |