Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers
Randomly amplified polymorphic DNA (RAPD) markers fingerprinting were used to assess the level of genetic variations among 24 cut-flower Anthurium andraeanum Hort. cultivars. Eight decamer primersproduced a total of 98 reproducible PCR bands that were used to calculate the Nei and Li’s genetic dista...
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African Journal of Biotechnology
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ftjafricanj:oai:ojs.ajol.info:article/71344 2023-05-15T14:15:43+02:00 Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers P Nowbuth G Khittoo T Bahorun S Venkatasamy 2011-10-28 application/pdf http://ajol.info/index.php/ajb/article/view/71344 en eng African Journal of Biotechnology http://ajol.info/index.php/ajb/article/view/71344 Copyright for articles published in this journal is retained by the journal. African Journal of Biotechnology; Vol 4, No 10 (2005) Anthurium andraeanum Hort. genetic variation genetic distance RAPD fingerprinting UPGMA dendrogram spathe Peer-reviewed Article 2011 ftjafricanj 2011-10-30T00:54:05Z Randomly amplified polymorphic DNA (RAPD) markers fingerprinting were used to assess the level of genetic variations among 24 cut-flower Anthurium andraeanum Hort. cultivars. Eight decamer primersproduced a total of 98 reproducible PCR bands that were used to calculate the Nei and Li’s genetic distance (GDNL) coefficients amongst the cultivars. GDNL values ranged from 0.018 to 0.163 with an average of 0.09 (representing an average genetic similarity of 91.34%). This significantly low average genetic distance among the various cultivars indicated that genetic variation among the cultivars was low. A dendrogram, produced using unweighted pair group method using arithmetic averages (UPGMA), grouped the cultivars into four main clusters. Cultivar ‘Antartica’ was genetically distinct from all the others. ‘Midori’ and ‘Bourgogne’ together formed a cluster whereas the remaining 21 cultivars grouped into two clusters and were closely related to each other. Clusters did not relate to cultivar provenance or origin and were independent of floral colour and spathe category. Finding correlations between these morphological traits to RAPD markers would necessitate extensive primer screening. Nevertheless, RAPD markers fingerprinting allowed a rapid assessment of the level of genetic variation that would otherwise be difficult to evaluate using the limited number of morphological markers present among these closely related anthurium cultivars. Article in Journal/Newspaper antartic* AJOL - African Journals Online Midori ENVELOPE(39.600,39.600,-69.017,-69.017) |
institution |
Open Polar |
collection |
AJOL - African Journals Online |
op_collection_id |
ftjafricanj |
language |
English |
topic |
Anthurium andraeanum Hort. genetic variation genetic distance RAPD fingerprinting UPGMA dendrogram spathe |
spellingShingle |
Anthurium andraeanum Hort. genetic variation genetic distance RAPD fingerprinting UPGMA dendrogram spathe P Nowbuth G Khittoo T Bahorun S Venkatasamy Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers |
topic_facet |
Anthurium andraeanum Hort. genetic variation genetic distance RAPD fingerprinting UPGMA dendrogram spathe |
description |
Randomly amplified polymorphic DNA (RAPD) markers fingerprinting were used to assess the level of genetic variations among 24 cut-flower Anthurium andraeanum Hort. cultivars. Eight decamer primersproduced a total of 98 reproducible PCR bands that were used to calculate the Nei and Li’s genetic distance (GDNL) coefficients amongst the cultivars. GDNL values ranged from 0.018 to 0.163 with an average of 0.09 (representing an average genetic similarity of 91.34%). This significantly low average genetic distance among the various cultivars indicated that genetic variation among the cultivars was low. A dendrogram, produced using unweighted pair group method using arithmetic averages (UPGMA), grouped the cultivars into four main clusters. Cultivar ‘Antartica’ was genetically distinct from all the others. ‘Midori’ and ‘Bourgogne’ together formed a cluster whereas the remaining 21 cultivars grouped into two clusters and were closely related to each other. Clusters did not relate to cultivar provenance or origin and were independent of floral colour and spathe category. Finding correlations between these morphological traits to RAPD markers would necessitate extensive primer screening. Nevertheless, RAPD markers fingerprinting allowed a rapid assessment of the level of genetic variation that would otherwise be difficult to evaluate using the limited number of morphological markers present among these closely related anthurium cultivars. |
format |
Article in Journal/Newspaper |
author |
P Nowbuth G Khittoo T Bahorun S Venkatasamy |
author_facet |
P Nowbuth G Khittoo T Bahorun S Venkatasamy |
author_sort |
P Nowbuth |
title |
Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers |
title_short |
Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers |
title_full |
Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers |
title_fullStr |
Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers |
title_full_unstemmed |
Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers |
title_sort |
assessing genetic diversity of some anthurium andraeanum hort. cut-flower cultivars using rapd markers |
publisher |
African Journal of Biotechnology |
publishDate |
2011 |
url |
http://ajol.info/index.php/ajb/article/view/71344 |
long_lat |
ENVELOPE(39.600,39.600,-69.017,-69.017) |
geographic |
Midori |
geographic_facet |
Midori |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
African Journal of Biotechnology; Vol 4, No 10 (2005) |
op_relation |
http://ajol.info/index.php/ajb/article/view/71344 |
op_rights |
Copyright for articles published in this journal is retained by the journal. |
_version_ |
1766288020181352448 |