Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1
Purpose: To investigate the effect of azaindole on proliferation of liver cancer cells, as well as the underlying mechanism. Methods: Colony forming and 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assays were used to determine the effect of azaindole on cell proliferation. A...
Published in: | Tropical Journal of Pharmaceutical Research |
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Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
2022
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Online Access: | https://www.ajol.info/index.php/tjpr/article/view/219991 https://doi.org/10.4314/tjpr.v20i2.20 |
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ftjafricanj:oai:ajol.info:article/219991 2023-05-15T14:15:31+02:00 Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 You, Zhen Li, Bei Gao, Jun Lu, Jiong Xu, Ruihua 2022-01-13 application/pdf https://www.ajol.info/index.php/tjpr/article/view/219991 https://doi.org/10.4314/tjpr.v20i2.20 eng eng Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria https://www.ajol.info/index.php/tjpr/article/view/219991/207596 https://www.ajol.info/index.php/tjpr/article/view/219991 doi:10.4314/tjpr.v20i2.20 Tropical Journal of Pharmaceutical Research; Vol. 20 No. 2 (2021); 359-364 1596-9827 1596-5996 Liver cancer Azaindole Malignant tumor Kinesin Antartic sponge info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2022 ftjafricanj https://doi.org/10.4314/tjpr.v20i2.20 2022-01-16T01:21:40Z Purpose: To investigate the effect of azaindole on proliferation of liver cancer cells, as well as the underlying mechanism. Methods: Colony forming and 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assays were used to determine the effect of azaindole on cell proliferation. A tumor model was established through subcutaneous administration of HEPG2 cells to rats. Thereafter, in vivo tumor development was measured using Vernier caliper. Results: The proliferation potential of HEPG2 and SNU-398 cells was markedly and dose-dependently suppressed by treatment with azaindole at doses of 2, 4, 8, 16 and 20 μM (p < 0.05). The expression levels of Ki67 and PCNA levels were significantly down-regulated in HEPG2 and SNU-398 cells on treatment with 20 μM azaindole. Moreover, azaindole significantly suppressed mRNA and protein expressions of KIFC1 in HEPG2 and SNU-398 cells (p < 0.05). Tumor volume in azaindole-treated rats on day 21 was greatly reduced, while KIFC1 expression in azaindole-treated rat tumor tissue was significantly down-regulated, when compared to the model group (p < 0.05). Conclusion: Azaindole targets proliferation of liver cancer cells in vitro and inhibits tumor growth in vivo through a mechanism involving down-regulation of KIFCI expression. Thus, azaindole is a potential therapeutic candidate for liver cancer. Article in Journal/Newspaper antartic* AJOL - African Journals Online Tropical Journal of Pharmaceutical Research 20 2 359 364 |
institution |
Open Polar |
collection |
AJOL - African Journals Online |
op_collection_id |
ftjafricanj |
language |
English |
topic |
Liver cancer Azaindole Malignant tumor Kinesin Antartic sponge |
spellingShingle |
Liver cancer Azaindole Malignant tumor Kinesin Antartic sponge You, Zhen Li, Bei Gao, Jun Lu, Jiong Xu, Ruihua Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 |
topic_facet |
Liver cancer Azaindole Malignant tumor Kinesin Antartic sponge |
description |
Purpose: To investigate the effect of azaindole on proliferation of liver cancer cells, as well as the underlying mechanism. Methods: Colony forming and 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assays were used to determine the effect of azaindole on cell proliferation. A tumor model was established through subcutaneous administration of HEPG2 cells to rats. Thereafter, in vivo tumor development was measured using Vernier caliper. Results: The proliferation potential of HEPG2 and SNU-398 cells was markedly and dose-dependently suppressed by treatment with azaindole at doses of 2, 4, 8, 16 and 20 μM (p < 0.05). The expression levels of Ki67 and PCNA levels were significantly down-regulated in HEPG2 and SNU-398 cells on treatment with 20 μM azaindole. Moreover, azaindole significantly suppressed mRNA and protein expressions of KIFC1 in HEPG2 and SNU-398 cells (p < 0.05). Tumor volume in azaindole-treated rats on day 21 was greatly reduced, while KIFC1 expression in azaindole-treated rat tumor tissue was significantly down-regulated, when compared to the model group (p < 0.05). Conclusion: Azaindole targets proliferation of liver cancer cells in vitro and inhibits tumor growth in vivo through a mechanism involving down-regulation of KIFCI expression. Thus, azaindole is a potential therapeutic candidate for liver cancer. |
format |
Article in Journal/Newspaper |
author |
You, Zhen Li, Bei Gao, Jun Lu, Jiong Xu, Ruihua |
author_facet |
You, Zhen Li, Bei Gao, Jun Lu, Jiong Xu, Ruihua |
author_sort |
You, Zhen |
title |
Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 |
title_short |
Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 |
title_full |
Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 |
title_fullStr |
Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 |
title_full_unstemmed |
Azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member C1 |
title_sort |
azaindole inhibits liver cancer cell proliferation in vitro and in vivo by targeting the expression of kinesin family member c1 |
publisher |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria |
publishDate |
2022 |
url |
https://www.ajol.info/index.php/tjpr/article/view/219991 https://doi.org/10.4314/tjpr.v20i2.20 |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
Tropical Journal of Pharmaceutical Research; Vol. 20 No. 2 (2021); 359-364 1596-9827 1596-5996 |
op_relation |
https://www.ajol.info/index.php/tjpr/article/view/219991/207596 https://www.ajol.info/index.php/tjpr/article/view/219991 doi:10.4314/tjpr.v20i2.20 |
op_doi |
https://doi.org/10.4314/tjpr.v20i2.20 |
container_title |
Tropical Journal of Pharmaceutical Research |
container_volume |
20 |
container_issue |
2 |
container_start_page |
359 |
op_container_end_page |
364 |
_version_ |
1766287877646319616 |