Cellular immunity in women aged 20–40 living in different climatic and geographic regions

• Published in: Archives of Disease in Childhood - Fetal and Neonatal Edition
• Main Authors: L. S. Shchegoleva, E. Yu. Shashkova, T. B. Sergeeva, Л. С. Щёголева, Е. Ю. Шашкова, Т. Б. Сергеева
• Other Authors: Исследование выполнено при финансовой поддержке госзадания «Физиологическая значимость особенностей иммунного гомеостаза, функциональной и рецепторной активности иммунокомпетентных клеток у людей в экстремальных меняющихся условиях среды с учётом профессионального статуса и социально-значимых заболеваний у жителей Приарктического региона» (№ государственной регистрации 122011700267-5)
• Format: Article in Journal/Newspaper
• Language: Russian
• Published: Scientific Centre for Family Health and Human Reproduction Problems 2024
• Subjects: резервные возможности иммунного гомеостаза; southern region; adaptive immunity; cytokines; reserve capabilities of immune homeostasis; южный регион; адаптивный иммунитет; цитокины; Arctic; Arkhangelsk; Архангельск*
• Online Access: https://www.actabiomedica.ru/jour/article/view/4734; https://doi.org/10.29413/ABS.2024-9.2.12

The aim. To identify phenotypic features of adaptive cellular immune responses in young women living in different climatic and geographic regions.Materials and methods. We examined 63 apparently healthy women, including 25 residents of Sovpolye settlement, Arkhangelsk region (Arctic region) and 38 residents of Tskhinvali (Republic of South Ossetia) aged 20–40. The content of lymphocytes, their phenotypes CD4+, CD8+, CD3+, CD5+, CD16+, CD10+, CD71+, CD25+, HLA-DR+ and CD95+, CEA (carcinoembryonic antigen) glycoprotein, and interleukin (IL) 6 and IL-10 cytokines was studied. Lymphocytes phenotyping was performed by indirect immunoperoxidase reaction using monoclonal antibodies (MedBioSpektr, Moscow) on “dried drop” lymphocyte sample with peroxidase conjugate and chromogen solution staining for immersion microscopy analysis (Nicon 50i, Japan). The content of CEA glycoprotein and cytokines was determined using ELISA.Results. In women living in Arctic region, a deficiency of CD5+, CD3+, CD10+, CD95+, CD71+, CD25+, HLA-DR+ cells, CEA and IL-6 concentrations is associated with high concentrations of CD8+ and IL-10. In women living in Southern region, a slight deficiency of CD5+, CD8+ and CD95+ cells is associated with high concentrations of CEA, IL-6, IL-10 and CD10+, CD16+ and HLA-DR+ cells.Conclusion. In inhabitants of different climatic regions, the features of formation of adaptive immune reactions are determined by different quantitative and qualitative composition of lymphocytes and cytokines phenotypes. Reduction of reserve capabilities of immune homeostasis is detected 3 times more often in inhabitants of the Arctic region. Цель исследования. Выявить фенотипические особенности адаптивных клеточных иммунных реакций у молодых женщин, проживающих в разных климатогеографических регионах.Материалы и методы исследования. Обследовано 63 практически здоровых женщины, в том числе 25 жительниц п. Совполье Архангельской области (Арктический регион) и 38 жительниц г. Цхинвал (Республика Южная Осетия) в возрасте 20–40 ...