Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks
Introduction.On the territory of the Ekhirit-Bulagatsky district of the Irkutsk region zones of sympatry of four Ixodes ticks species are found, where the species and genetic diversity of infectious agents transmitted through tick bites may be more pronounced than in foci with a mono-dominant type o...
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Scientific Centre for Family Health and Human Reproduction Problems
2019
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Online Access: | https://www.actabiomedica.ru/jour/article/view/2056 https://doi.org/10.29413/ABS.2019-4.2.18 |
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ftjabs:oai:oai.actabiomedica.elpub.ru:article/2056 |
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Acta Biomedica Scientifica |
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ftjabs |
language |
Russian |
topic |
полимеразная цепная реакция Ehrlichia ixodic ticks reservoir hosts polymerase chain reaction эрлихии иксодовые клещи резервуарные хозяева |
spellingShingle |
полимеразная цепная реакция Ehrlichia ixodic ticks reservoir hosts polymerase chain reaction эрлихии иксодовые клещи резервуарные хозяева E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova Е. К. Дорощенко О. В. Лисак В. А. Рар О. В. Сунцова Ю. С. Савинова И. В. Козлова Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks |
topic_facet |
полимеразная цепная реакция Ehrlichia ixodic ticks reservoir hosts polymerase chain reaction эрлихии иксодовые клещи резервуарные хозяева |
description |
Introduction.On the territory of the Ekhirit-Bulagatsky district of the Irkutsk region zones of sympatry of four Ixodes ticks species are found, where the species and genetic diversity of infectious agents transmitted through tick bites may be more pronounced than in foci with a mono-dominant type of ticks’ population. In this connection, the study of the species and genetic diversity of representatives of the Anaplasmataceae family in the sympatry zone of the Ixodes ticks of closely related species was of scientific interest.Objective: To study the species and genetic diversity of members of the Anaplasmataceae family in the zones of sympatry of Ixodes ticks Ixodes persulcatus, Dermacentor silvarum, D. nuttalli and Haemaphysalis concinna, to identify the main carriers and potential reservoir hosts of ehrlichia and anaplasma.Methods. In the course of the study, 1106 specimens of adult ticks and 49 samples of small mammalian livers from the Ekhirit-Bulagatsky area were analyzed. Anaplasma and ehrlichia DNA were detected by two-round PCR in the presence of genus- and species-specific primers from the 16S rRNA gene region. The nucleotide sequences of the 16S rRNA gene and the fragment of the groESL operon were identified in some samples. Sequencing was carried out according to the Sanger method. Comparative analysis was performed using the BLASTN program and ClustalW method. Epidemiological data analysis was performed using parametric methods of statistical processing of the material.Results. The DNA of Ehrlichia muris and Anaplasma phagocytophilum were detected in all studied species of ticks in their sympatry area. However, the rate of infection of taiga ticks was significantly higher than that of H. concinna and Dermacentor spp. Potential reservoir hosts of the Anaplasmataceae family members can be classified as Microtus oeconomus, M. gregalis, Myodes rutilus and Sorex spp. When analyzing the nucleotide sequences of the 16S rRNA gene, three genetic variants of anaplasma were detected. The nucleotide sequences of ... |
author2 |
грант РФФИ № 16-04-01336_а. |
format |
Article in Journal/Newspaper |
author |
E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova Е. К. Дорощенко О. В. Лисак В. А. Рар О. В. Сунцова Ю. С. Савинова И. В. Козлова |
author_facet |
E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova Е. К. Дорощенко О. В. Лисак В. А. Рар О. В. Сунцова Ю. С. Савинова И. В. Козлова |
author_sort |
E. K. Doroshchenko |
title |
Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks |
title_short |
Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks |
title_full |
Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks |
title_fullStr |
Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks |
title_full_unstemmed |
Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks |
title_sort |
species and genetic diversity of representatives of the anaplasmataceae family found in the sympatry zone of the ixodes, dermacentor and haemaphysalis genera ticks |
publisher |
Scientific Centre for Family Health and Human Reproduction Problems |
publishDate |
2019 |
url |
https://www.actabiomedica.ru/jour/article/view/2056 https://doi.org/10.29413/ABS.2019-4.2.18 |
genre |
taiga |
genre_facet |
taiga |
op_source |
Acta Biomedica Scientifica; Том 4, № 2 (2019); 127-135 2587-9596 2541-9420 |
op_relation |
https://www.actabiomedica.ru/jour/article/view/2056/1873 Филиппова Н.А. Симпатрия близкородственных видов иксодовых клещей и её возможная роль в паразитарных системах природных очагов трансмиссивных болезней. Паразитология. 1999; 33(3): 223-241. Козлова И.В., Злобин В.И., Верхозина М.М., Рар В.А., Лисак О.В., Дорощенко Е.К., и др. Результаты рекогносцировочных исследований по моноцитарному эрлихиозу и гранулоцитарному анаплазмозу человека в Прибайкалье. Acta biomedica scientifica. 2007; 3(55): 112-116. Козлова И.В., Верхозина М.М., Дёмина Т.В., Джиоев Ю.П., Дорощенко Е.К., Лисак О.В., и др. Сочетанные очаги трансмиссивных клещевых инфекций на территории Прибайкалья. Эпидемиология и вакцинопрофилактика. 2010; 4(53): 40-46. Ливанова Н.Н., Рар В.А., Ливанов С.Г., Иголкина Я.П. Разнообразие паразитарных систем с участием мелких млекопитающих и Ixodes persulcatus Shculze на Северном Урале. Сибирский экологический журнал. 2005; (6): 1079-1084. Рар В.А., Пуховская Н.М., Высочина Н.П., Зайнулина З.У., Гуляко Л.Ф., Иванов Л.И. Распространение и генетическое разнообразие эрлихий и анаплазм в таежных клещах и мелких млекопитающих на территории Хабаровского края. Acta biomedica scientifica. 2007; 3 (Приложение): 156-159. Rar VA, Livanova NN, Panov VV, Kozlova IV, Pukhovskaya NM, Vysochina NP, et al. Prevalence of Anaplasmaand Ehrlichia species in Ixodes persulcatus ticks and small mammals from different regions of Asian part of Russia. Int J Med Microbiol. 2008; 298(1): 222-230. doi:10.1016/j.ijmm.2008.01.001 Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, et al. Tick-borne pathogen detection, Western Sibiria, Russia. Emerg Infect Dis. 2005; 11: 1708-1715. Rar VA, Epikhina TI, Livanova NN, Panov VV, Doroshchenko EK, Pukhovskaya NM, et al. The study of heterogeneity of 16S rRNA gene and groESL operone in DNA samples of Anaplasma phagocytophilum, Ehrlichia muris, and “Candidatus Neoehrlichia mikurensis” determined in Ixodes persulcatus ticks on the territory of Ural, Siberia and Far East of Russia. Mol Gen Microbiol Virol. 2011; 26(2): 66-73. doi:10.3103/S0891416811020091 Sumner JW, Nicholson WL, Massung RF. PCR amplification and comparison of nucleotide sequences from the groESLheat shock operon of Ehrlichiaspecies. J Clin Microbiol. 1997; 35(8): 2087-2092. Liz JS, Anderes L, Sumner JW, Massung RF, Gern L, Rutti B, et al. PCR detection of granulocytic ehrlichia in Ixodes ricinusticks and wild small mammals in western Switzerland. J Clin Microbiol. 2000; 38(3): 1002-1007. Савилов Е.Д., Астафьев В.А., Жданова С.Н., Заруднев Е.А. Эпидемиологический анализ: Методы статистиче ской обработки материала. Новосибирск: Наука-Центр; 2011. Rar VA, Livanova NN, Panov VV, Doroshchenko EK, Pukhovskaya NM, Vysochina NP, et al. Genetic diversity of Anaplasma and Ehrlichiain Asian part of Russia. Ticks TickBorne Dis.2010; 1(1): 57-65. doi:10.1016/j.ttbdis.2010.01.002 Rar VA, Epikhina TI, Livanova NN, Panov VV, Doroschenko EK, Pukhovskaya NM, et al. Genetic variability of Anaplasma phagocytophilumin Ixodes persulcatusticks and small mammals in the Asian part of Russia. VectorBorne Zoonot Dis.2011; 11(8): 1013-1021. doi:10.1089/vbz.2010.0266 Kawahara M, Suto C, Rikihisa Y, Yamamoto S, Tsuboi Y. Characterization of ehrlichial organisms isolated from a wild mouse. J Clin Microbiol. 1993; (31): 89-96. Wen B, Rikihisa Y, Mott J, Fuerst PA, Kawahara M, Suto C. Ehrlichia muris sp. nov., identified on the basis of 16S rRNA base sequences and serological, morphological and biological characteristics. Int J Syst Bacteriol. 1995: (45): 250-254. doi:10.1099/00207713-45-2-250 Kawahara M, Ito T, Suto C, Shibata S, Rikihisa Y, Hata K, et al. Comparison of Ehrlichia murisstrains isolated from wild mice and ticks and serologic evidence of humans and animals with E. murisas antigen. J Clin Microbiol. 1999: 37(4): 1123-1129. Sumner JW, Storch GA, Buller RS, Liddell AM, Stockham SL, Rikihisa Y, et al. PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris. J Clin Microbiol. 2000; 38(7): 2746-2749. Von Loewenich FD, Stumpf G, Baumgarten BU, Rollinghoff M, Dumler JS, Bogdan C. A case of equine granulocytic ehrlichiosis provides molecular evidence for the presence of pathogenic Anaplasma phagocytophilum(HGE agent) in Germany. Eur J Clin Microbiol Infect Dis. 2003; 22(5): 303-305. doi:10.1007/s10096-003-0935-1 Cao WC, Zhan L, He J, Foley J, De Vlas SJ, Wu XM, Yang H, Richardus JH, Habbema JD. Natural Anaplasma phagocytophilum infection of ticks and rodents from a forest area of Jilin Province, China. Am J Trop Med Hyg. 2006; 75(4): 664-668. Cao WC, Zhao QM, Zhang PH, Dumler JS, Zhang XT, Fang LQ, et al. Granulocytic ehrlichia in Ixodes persulcatusticks from an area in China where Lyme disease is endemic. J Clin Microbiol. 2000; 38(11): 4208-4210. Rar VA, Golovljova I. Anaplasma, Ehrlichia, and “Candidatus Neoehrlichia” bacteria: pathogenicity, biodiversity, and molecular genetic characteristics, a review. Infect Genet Evol. 2011; 11(8): 1842-1861. doi:10.1016/j.meegid.2011.09.019 Rar VA, Epikhina TI, Tikunova NV, Yakimenko VV, Malkova MG, Tancev AK, et al. Genetic variability of Anaplasma phagocy tophilum in ticks and voles from Ixodes persulcatus/ Ixodes triangu liceps sympatric areas from Western Siberia, Russia. Ticks TickBorne Dis. 2014; 5(6): 854-863. doi:10.1016/j.ttbdis.2014.07.008 Blaňarová L, Stanko M, Carpi G, Miklisová D, Víchová B, Mošanský L, et al. Distinct Anaplasma phagocytophilumgenotypes associated with Ixodes triangulicepsticks and rodents in Central Europe. Ticks TickBorne Dis.2014; 5(6): 928-38. doi:10.1016/j.ttbdis.2014.07.012. https://www.actabiomedica.ru/jour/article/view/2056 doi:10.29413/ABS.2019-4.2.18 |
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ftjabs:oai:oai.actabiomedica.elpub.ru:article/2056 2023-05-15T18:31:05+02:00 Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks Видовое и генетическое разнообразие представителей семейства Anaplasmataceae, выявленное в зоне симпатрии клещей родов Ixodes, Dermacentor, Haemaphysalis E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova Е. К. Дорощенко О. В. Лисак В. А. Рар О. В. Сунцова Ю. С. Савинова И. В. Козлова грант РФФИ № 16-04-01336_а. 2019-05-25 application/pdf https://www.actabiomedica.ru/jour/article/view/2056 https://doi.org/10.29413/ABS.2019-4.2.18 rus rus Scientific Centre for Family Health and Human Reproduction Problems https://www.actabiomedica.ru/jour/article/view/2056/1873 Филиппова Н.А. Симпатрия близкородственных видов иксодовых клещей и её возможная роль в паразитарных системах природных очагов трансмиссивных болезней. Паразитология. 1999; 33(3): 223-241. Козлова И.В., Злобин В.И., Верхозина М.М., Рар В.А., Лисак О.В., Дорощенко Е.К., и др. Результаты рекогносцировочных исследований по моноцитарному эрлихиозу и гранулоцитарному анаплазмозу человека в Прибайкалье. Acta biomedica scientifica. 2007; 3(55): 112-116. Козлова И.В., Верхозина М.М., Дёмина Т.В., Джиоев Ю.П., Дорощенко Е.К., Лисак О.В., и др. Сочетанные очаги трансмиссивных клещевых инфекций на территории Прибайкалья. Эпидемиология и вакцинопрофилактика. 2010; 4(53): 40-46. Ливанова Н.Н., Рар В.А., Ливанов С.Г., Иголкина Я.П. Разнообразие паразитарных систем с участием мелких млекопитающих и Ixodes persulcatus Shculze на Северном Урале. Сибирский экологический журнал. 2005; (6): 1079-1084. Рар В.А., Пуховская Н.М., Высочина Н.П., Зайнулина З.У., Гуляко Л.Ф., Иванов Л.И. Распространение и генетическое разнообразие эрлихий и анаплазм в таежных клещах и мелких млекопитающих на территории Хабаровского края. Acta biomedica scientifica. 2007; 3 (Приложение): 156-159. Rar VA, Livanova NN, Panov VV, Kozlova IV, Pukhovskaya NM, Vysochina NP, et al. Prevalence of Anaplasmaand Ehrlichia species in Ixodes persulcatus ticks and small mammals from different regions of Asian part of Russia. Int J Med Microbiol. 2008; 298(1): 222-230. doi:10.1016/j.ijmm.2008.01.001 Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, et al. Tick-borne pathogen detection, Western Sibiria, Russia. Emerg Infect Dis. 2005; 11: 1708-1715. Rar VA, Epikhina TI, Livanova NN, Panov VV, Doroshchenko EK, Pukhovskaya NM, et al. The study of heterogeneity of 16S rRNA gene and groESL operone in DNA samples of Anaplasma phagocytophilum, Ehrlichia muris, and “Candidatus Neoehrlichia mikurensis” determined in Ixodes persulcatus ticks on the territory of Ural, Siberia and Far East of Russia. Mol Gen Microbiol Virol. 2011; 26(2): 66-73. doi:10.3103/S0891416811020091 Sumner JW, Nicholson WL, Massung RF. PCR amplification and comparison of nucleotide sequences from the groESLheat shock operon of Ehrlichiaspecies. J Clin Microbiol. 1997; 35(8): 2087-2092. Liz JS, Anderes L, Sumner JW, Massung RF, Gern L, Rutti B, et al. PCR detection of granulocytic ehrlichia in Ixodes ricinusticks and wild small mammals in western Switzerland. J Clin Microbiol. 2000; 38(3): 1002-1007. Савилов Е.Д., Астафьев В.А., Жданова С.Н., Заруднев Е.А. Эпидемиологический анализ: Методы статистиче ской обработки материала. Новосибирск: Наука-Центр; 2011. Rar VA, Livanova NN, Panov VV, Doroshchenko EK, Pukhovskaya NM, Vysochina NP, et al. Genetic diversity of Anaplasma and Ehrlichiain Asian part of Russia. Ticks TickBorne Dis.2010; 1(1): 57-65. doi:10.1016/j.ttbdis.2010.01.002 Rar VA, Epikhina TI, Livanova NN, Panov VV, Doroschenko EK, Pukhovskaya NM, et al. Genetic variability of Anaplasma phagocytophilumin Ixodes persulcatusticks and small mammals in the Asian part of Russia. VectorBorne Zoonot Dis.2011; 11(8): 1013-1021. doi:10.1089/vbz.2010.0266 Kawahara M, Suto C, Rikihisa Y, Yamamoto S, Tsuboi Y. Characterization of ehrlichial organisms isolated from a wild mouse. J Clin Microbiol. 1993; (31): 89-96. Wen B, Rikihisa Y, Mott J, Fuerst PA, Kawahara M, Suto C. Ehrlichia muris sp. nov., identified on the basis of 16S rRNA base sequences and serological, morphological and biological characteristics. Int J Syst Bacteriol. 1995: (45): 250-254. doi:10.1099/00207713-45-2-250 Kawahara M, Ito T, Suto C, Shibata S, Rikihisa Y, Hata K, et al. Comparison of Ehrlichia murisstrains isolated from wild mice and ticks and serologic evidence of humans and animals with E. murisas antigen. J Clin Microbiol. 1999: 37(4): 1123-1129. Sumner JW, Storch GA, Buller RS, Liddell AM, Stockham SL, Rikihisa Y, et al. PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris. J Clin Microbiol. 2000; 38(7): 2746-2749. Von Loewenich FD, Stumpf G, Baumgarten BU, Rollinghoff M, Dumler JS, Bogdan C. A case of equine granulocytic ehrlichiosis provides molecular evidence for the presence of pathogenic Anaplasma phagocytophilum(HGE agent) in Germany. Eur J Clin Microbiol Infect Dis. 2003; 22(5): 303-305. doi:10.1007/s10096-003-0935-1 Cao WC, Zhan L, He J, Foley J, De Vlas SJ, Wu XM, Yang H, Richardus JH, Habbema JD. Natural Anaplasma phagocytophilum infection of ticks and rodents from a forest area of Jilin Province, China. Am J Trop Med Hyg. 2006; 75(4): 664-668. Cao WC, Zhao QM, Zhang PH, Dumler JS, Zhang XT, Fang LQ, et al. Granulocytic ehrlichia in Ixodes persulcatusticks from an area in China where Lyme disease is endemic. J Clin Microbiol. 2000; 38(11): 4208-4210. Rar VA, Golovljova I. Anaplasma, Ehrlichia, and “Candidatus Neoehrlichia” bacteria: pathogenicity, biodiversity, and molecular genetic characteristics, a review. Infect Genet Evol. 2011; 11(8): 1842-1861. doi:10.1016/j.meegid.2011.09.019 Rar VA, Epikhina TI, Tikunova NV, Yakimenko VV, Malkova MG, Tancev AK, et al. Genetic variability of Anaplasma phagocy tophilum in ticks and voles from Ixodes persulcatus/ Ixodes triangu liceps sympatric areas from Western Siberia, Russia. Ticks TickBorne Dis. 2014; 5(6): 854-863. doi:10.1016/j.ttbdis.2014.07.008 Blaňarová L, Stanko M, Carpi G, Miklisová D, Víchová B, Mošanský L, et al. Distinct Anaplasma phagocytophilumgenotypes associated with Ixodes triangulicepsticks and rodents in Central Europe. Ticks TickBorne Dis.2014; 5(6): 928-38. doi:10.1016/j.ttbdis.2014.07.012. https://www.actabiomedica.ru/jour/article/view/2056 doi:10.29413/ABS.2019-4.2.18 Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access). 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CC-BY Acta Biomedica Scientifica; Том 4, № 2 (2019); 127-135 2587-9596 2541-9420 полимеразная цепная реакция Ehrlichia ixodic ticks reservoir hosts polymerase chain reaction эрлихии иксодовые клещи резервуарные хозяева info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftjabs https://doi.org/10.29413/ABS.2019-4.2.18 https://doi.org/10.1016/j.ijmm.2008.01.001 https://doi.org/10.3103/S0891416811020091 https://doi.org/10.1016/j.ttbdis.2010.01.002 https://doi.org/10.1089/vbz.2010.0266 https://doi.org/10.1099/00207713-45- 2022-10-02T06:08:54Z Introduction.On the territory of the Ekhirit-Bulagatsky district of the Irkutsk region zones of sympatry of four Ixodes ticks species are found, where the species and genetic diversity of infectious agents transmitted through tick bites may be more pronounced than in foci with a mono-dominant type of ticks’ population. In this connection, the study of the species and genetic diversity of representatives of the Anaplasmataceae family in the sympatry zone of the Ixodes ticks of closely related species was of scientific interest.Objective: To study the species and genetic diversity of members of the Anaplasmataceae family in the zones of sympatry of Ixodes ticks Ixodes persulcatus, Dermacentor silvarum, D. nuttalli and Haemaphysalis concinna, to identify the main carriers and potential reservoir hosts of ehrlichia and anaplasma.Methods. In the course of the study, 1106 specimens of adult ticks and 49 samples of small mammalian livers from the Ekhirit-Bulagatsky area were analyzed. Anaplasma and ehrlichia DNA were detected by two-round PCR in the presence of genus- and species-specific primers from the 16S rRNA gene region. The nucleotide sequences of the 16S rRNA gene and the fragment of the groESL operon were identified in some samples. Sequencing was carried out according to the Sanger method. Comparative analysis was performed using the BLASTN program and ClustalW method. Epidemiological data analysis was performed using parametric methods of statistical processing of the material.Results. The DNA of Ehrlichia muris and Anaplasma phagocytophilum were detected in all studied species of ticks in their sympatry area. However, the rate of infection of taiga ticks was significantly higher than that of H. concinna and Dermacentor spp. Potential reservoir hosts of the Anaplasmataceae family members can be classified as Microtus oeconomus, M. gregalis, Myodes rutilus and Sorex spp. When analyzing the nucleotide sequences of the 16S rRNA gene, three genetic variants of anaplasma were detected. The nucleotide sequences of ... Article in Journal/Newspaper taiga Acta Biomedica Scientifica Acta Biomedica Scientifica (East Siberian Biomedical Journal) 4 2 127 135 |