Histopathological investigation of complex gill disease in sea farmed Atlantic salmon
Various agents including Ca. Piscichlamydia salmonis, Ca. Branchiomonas cysticola, Des-mozoon lepeophtherii, Paramoeba perurans and salmon gill poxvirus may be associated with complex gill disease in Atlantic salmon. Co-infections involving two or more of these agents are common and histopathologica...
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ftistanbuluniv:oai:http://acikerisim.istanbul.edu.tr:20.500.12627/95203 2023-05-15T15:31:03+02:00 Histopathological investigation of complex gill disease in sea farmed Atlantic salmon Colquhoun, Duncan J. Steinum, Terje Marken Olsen, Anne Berit Lie, Kai Inge Tavornpanich, Saraya Gjevre, Anne-Gerd Gjessing, Mona C. Norwegian Veterinary Institute , , 2485212 2019 https://hdl.handle.net/20.500.12627/95203 https://doi.org/10.1371/journal.pone.0222926 eng eng PLOS ONE Gjessing M. C. , Steinum T. M. , Olsen A. B. , Lie K. I. , Tavornpanich S., Colquhoun D. J. , Gjevre A., "Histopathological investigation of complex gill disease in sea farmed Atlantic salmon", PLOS ONE, cilt.14, 2019 1932-6203 vv_1032021 av_8cc17fb0-9fac-4fc6-b44f-dd2706c457cc http://hdl.handle.net/20.500.12627/95203 https://doi.org/10.1371/journal.pone.0222926 14 10 ÇOK DİSİPLİNLİ BİLİMLER Doğa Bilimleri Genel Temel Bilimler (SCI) Multidisciplinary Temel Bilimler Makale 2019 ftistanbuluniv https://doi.org/20.500.12627/95203 https://doi.org/10.1371/journal.pone.0222926 2022-08-10T13:39:04Z Various agents including Ca. Piscichlamydia salmonis, Ca. Branchiomonas cysticola, Des-mozoon lepeophtherii, Paramoeba perurans and salmon gill poxvirus may be associated with complex gill disease in Atlantic salmon. Co-infections involving two or more of these agents are common and histopathological interpretation of lesions is therefore challenging. In this study, we developed a semi-quantitative scoring system for examination of histopathological gill lesions in sea-farmed Atlantic salmon suffering from gill disease. Following qPCR analysis of gills sampled for Ca. P. salmonis, Ca. B. cysticola, D. lepeophtherii and P. perurans from 22 geographically spread outbreaks, five cases representing different infectious loads and combinations of agents were chosen for histopathological scoring. Twenty-eight histological features were evaluated and potential associations between individual pathological changes and the occurrence of individual agents studied. The inter-observer agreement in interpretation of histological parameters between the three pathologists involved, was calculated to validate robustness of the scoring scheme. Seventeen histological parameters met the criteria for inter-observer agreement analysis and were included in the calculation. The three most frequent findings were identification of subepithelial leukocytes, epithelial cell hyperplasia and mucus cell hyperplasia. While few findings could be specifically related to particular agents, necrosis in hyperplastic lesions, pustules and necrosis of subepithelial cells appeared to be associated with the presence of Ca. B. cysticola. Further, lesion profiles clearly support the previously identified association between P. perurans and pathological changes associated with AGD. Very few pathological changes were observed in the single case in which Ca. P. salmonis was the dominating agent. Some lesions were only very rarely observed e.g. chloride cell necrosis, epithelial cell apoptosis, lamellar deposition of melanin and haemophagocytosis. The scoring ... Article in Journal/Newspaper Atlantic salmon İstanbul Üniversitesi Açık Erişim Sistemi PLOS ONE 14 10 e0222926 |
institution |
Open Polar |
collection |
İstanbul Üniversitesi Açık Erişim Sistemi |
op_collection_id |
ftistanbuluniv |
language |
English |
topic |
ÇOK DİSİPLİNLİ BİLİMLER Doğa Bilimleri Genel Temel Bilimler (SCI) Multidisciplinary Temel Bilimler |
spellingShingle |
ÇOK DİSİPLİNLİ BİLİMLER Doğa Bilimleri Genel Temel Bilimler (SCI) Multidisciplinary Temel Bilimler Colquhoun, Duncan J. Steinum, Terje Marken Olsen, Anne Berit Lie, Kai Inge Tavornpanich, Saraya Gjevre, Anne-Gerd Gjessing, Mona C. Histopathological investigation of complex gill disease in sea farmed Atlantic salmon |
topic_facet |
ÇOK DİSİPLİNLİ BİLİMLER Doğa Bilimleri Genel Temel Bilimler (SCI) Multidisciplinary Temel Bilimler |
description |
Various agents including Ca. Piscichlamydia salmonis, Ca. Branchiomonas cysticola, Des-mozoon lepeophtherii, Paramoeba perurans and salmon gill poxvirus may be associated with complex gill disease in Atlantic salmon. Co-infections involving two or more of these agents are common and histopathological interpretation of lesions is therefore challenging. In this study, we developed a semi-quantitative scoring system for examination of histopathological gill lesions in sea-farmed Atlantic salmon suffering from gill disease. Following qPCR analysis of gills sampled for Ca. P. salmonis, Ca. B. cysticola, D. lepeophtherii and P. perurans from 22 geographically spread outbreaks, five cases representing different infectious loads and combinations of agents were chosen for histopathological scoring. Twenty-eight histological features were evaluated and potential associations between individual pathological changes and the occurrence of individual agents studied. The inter-observer agreement in interpretation of histological parameters between the three pathologists involved, was calculated to validate robustness of the scoring scheme. Seventeen histological parameters met the criteria for inter-observer agreement analysis and were included in the calculation. The three most frequent findings were identification of subepithelial leukocytes, epithelial cell hyperplasia and mucus cell hyperplasia. While few findings could be specifically related to particular agents, necrosis in hyperplastic lesions, pustules and necrosis of subepithelial cells appeared to be associated with the presence of Ca. B. cysticola. Further, lesion profiles clearly support the previously identified association between P. perurans and pathological changes associated with AGD. Very few pathological changes were observed in the single case in which Ca. P. salmonis was the dominating agent. Some lesions were only very rarely observed e.g. chloride cell necrosis, epithelial cell apoptosis, lamellar deposition of melanin and haemophagocytosis. The scoring ... |
author2 |
Norwegian Veterinary Institute , , 2485212 |
format |
Article in Journal/Newspaper |
author |
Colquhoun, Duncan J. Steinum, Terje Marken Olsen, Anne Berit Lie, Kai Inge Tavornpanich, Saraya Gjevre, Anne-Gerd Gjessing, Mona C. |
author_facet |
Colquhoun, Duncan J. Steinum, Terje Marken Olsen, Anne Berit Lie, Kai Inge Tavornpanich, Saraya Gjevre, Anne-Gerd Gjessing, Mona C. |
author_sort |
Colquhoun, Duncan J. |
title |
Histopathological investigation of complex gill disease in sea farmed Atlantic salmon |
title_short |
Histopathological investigation of complex gill disease in sea farmed Atlantic salmon |
title_full |
Histopathological investigation of complex gill disease in sea farmed Atlantic salmon |
title_fullStr |
Histopathological investigation of complex gill disease in sea farmed Atlantic salmon |
title_full_unstemmed |
Histopathological investigation of complex gill disease in sea farmed Atlantic salmon |
title_sort |
histopathological investigation of complex gill disease in sea farmed atlantic salmon |
publishDate |
2019 |
url |
https://hdl.handle.net/20.500.12627/95203 https://doi.org/10.1371/journal.pone.0222926 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
PLOS ONE Gjessing M. C. , Steinum T. M. , Olsen A. B. , Lie K. I. , Tavornpanich S., Colquhoun D. J. , Gjevre A., "Histopathological investigation of complex gill disease in sea farmed Atlantic salmon", PLOS ONE, cilt.14, 2019 1932-6203 vv_1032021 av_8cc17fb0-9fac-4fc6-b44f-dd2706c457cc http://hdl.handle.net/20.500.12627/95203 https://doi.org/10.1371/journal.pone.0222926 14 10 |
op_doi |
https://doi.org/20.500.12627/95203 https://doi.org/10.1371/journal.pone.0222926 |
container_title |
PLOS ONE |
container_volume |
14 |
container_issue |
10 |
container_start_page |
e0222926 |
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1766361547174576128 |