An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation
Proliferative gill inflammation, a respiratory disease of apparently multifactorial aetiology, affects seawater-farmed Atlantic salmon in Norway and may be associated with significant losses. In the present study, the gill-associated bacterial community in fish suffering PGI as diagnosed by histolog...
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Online Access: | https://hdl.handle.net/20.500.12627/69954 https://doi.org/10.1016/j.aquaculture.2009.05.006 |
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ftistanbuluniv:oai:http://acikerisim.istanbul.edu.tr:20.500.12627/69954 2024-06-23T07:51:21+00:00 An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation FALK, K. Steinum, Terje Marken KVELLESTAD, A. COLQUHOUN, D. J. SJASTAD, K. Norwegian University of Science & Technology (NTNU) , , 100547 2021-03-04T08:41:37Z https://hdl.handle.net/20.500.12627/69954 https://doi.org/10.1016/j.aquaculture.2009.05.006 eng eng AQUACULTURE Steinum T. M. , SJASTAD K., FALK K., KVELLESTAD A., COLQUHOUN D. J. , "An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation", AQUACULTURE, cilt.293, ss.172-179, 2009 0044-8486 vv_1032021 av_6485d8f0-fa8a-4bcb-b940-032c57eeda58 http://hdl.handle.net/20.500.12627/69954 https://doi.org/10.1016/j.aquaculture.2009.05.006 293 172 179 Toprak ve Su Muhafazası ve Amenajmanı Su Hasadı Su Ürünleri Ziraat Toprak ve Bitki Besleme Tarımsal Bilimler DENİZ VE TATLISU BİYOLOJİSİ Tarım ve Çevre Bilimleri (AGE) Bitki ve Hayvan Bilimleri BALIKÇILIK Makale 2021 ftistanbuluniv https://doi.org/20.500.12627/6995410.1016/j.aquaculture.2009.05.006 2024-06-06T04:10:03Z Proliferative gill inflammation, a respiratory disease of apparently multifactorial aetiology, affects seawater-farmed Atlantic salmon in Norway and may be associated with significant losses. In the present study, the gill-associated bacterial community in fish suffering PGI as diagnosed by histology was compared with that of clinically healthy fish by Reverse Transcriptase Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis. 'Candidatus Piscichlamydia salmonis', which forms histologically detectable intracellular inclusions commonly called epitheliocysts, was identified in 16 of 21 fish displaying PGI, normally as the dominant phylotype. Although identified by RT-DGGE in many clinically healthy fish from PGI affected farms, this bacterium was only detected in 7 of 48 fish from PGI-free farms, of which all positives interestingly originated from a single farm. Taken together, these results reveal a positive association between 'Candidatus Piscichlamydia salmonis' and PGI in the investigated material from 10 geographically distinct farms. Furthermore, a wide distribution of gamma-proteobacteria was detected, but the bacterial communities individual fish gills were generally quite simple and often dominated by one or two phylotypes, most commonly Burkholderia-like bacteria (previously Pseudomonads) and Psychrobacter spp. Representatives spoilage or fish pathogenic genera e.g. Photobacterium, Flavobacterium, Allivibrio, Shewanella and Tenacibaculum, were less frequently identified and apparently in low numbers. Sequences representing at least one undescribed species closely related to fish pathogenic Francisella spp. were also identified. (C) 2009 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Atlantic salmon İstanbul Üniversitesi Açık Erişim Sistemi Norway Aquaculture 293 3-4 172 179 |
institution |
Open Polar |
collection |
İstanbul Üniversitesi Açık Erişim Sistemi |
op_collection_id |
ftistanbuluniv |
language |
English |
topic |
Toprak ve Su Muhafazası ve Amenajmanı Su Hasadı Su Ürünleri Ziraat Toprak ve Bitki Besleme Tarımsal Bilimler DENİZ VE TATLISU BİYOLOJİSİ Tarım ve Çevre Bilimleri (AGE) Bitki ve Hayvan Bilimleri BALIKÇILIK |
spellingShingle |
Toprak ve Su Muhafazası ve Amenajmanı Su Hasadı Su Ürünleri Ziraat Toprak ve Bitki Besleme Tarımsal Bilimler DENİZ VE TATLISU BİYOLOJİSİ Tarım ve Çevre Bilimleri (AGE) Bitki ve Hayvan Bilimleri BALIKÇILIK FALK, K. Steinum, Terje Marken KVELLESTAD, A. COLQUHOUN, D. J. SJASTAD, K. An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation |
topic_facet |
Toprak ve Su Muhafazası ve Amenajmanı Su Hasadı Su Ürünleri Ziraat Toprak ve Bitki Besleme Tarımsal Bilimler DENİZ VE TATLISU BİYOLOJİSİ Tarım ve Çevre Bilimleri (AGE) Bitki ve Hayvan Bilimleri BALIKÇILIK |
description |
Proliferative gill inflammation, a respiratory disease of apparently multifactorial aetiology, affects seawater-farmed Atlantic salmon in Norway and may be associated with significant losses. In the present study, the gill-associated bacterial community in fish suffering PGI as diagnosed by histology was compared with that of clinically healthy fish by Reverse Transcriptase Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis. 'Candidatus Piscichlamydia salmonis', which forms histologically detectable intracellular inclusions commonly called epitheliocysts, was identified in 16 of 21 fish displaying PGI, normally as the dominant phylotype. Although identified by RT-DGGE in many clinically healthy fish from PGI affected farms, this bacterium was only detected in 7 of 48 fish from PGI-free farms, of which all positives interestingly originated from a single farm. Taken together, these results reveal a positive association between 'Candidatus Piscichlamydia salmonis' and PGI in the investigated material from 10 geographically distinct farms. Furthermore, a wide distribution of gamma-proteobacteria was detected, but the bacterial communities individual fish gills were generally quite simple and often dominated by one or two phylotypes, most commonly Burkholderia-like bacteria (previously Pseudomonads) and Psychrobacter spp. Representatives spoilage or fish pathogenic genera e.g. Photobacterium, Flavobacterium, Allivibrio, Shewanella and Tenacibaculum, were less frequently identified and apparently in low numbers. Sequences representing at least one undescribed species closely related to fish pathogenic Francisella spp. were also identified. (C) 2009 Elsevier B.V. All rights reserved. |
author2 |
Norwegian University of Science & Technology (NTNU) , , 100547 |
format |
Article in Journal/Newspaper |
author |
FALK, K. Steinum, Terje Marken KVELLESTAD, A. COLQUHOUN, D. J. SJASTAD, K. |
author_facet |
FALK, K. Steinum, Terje Marken KVELLESTAD, A. COLQUHOUN, D. J. SJASTAD, K. |
author_sort |
FALK, K. |
title |
An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation |
title_short |
An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation |
title_full |
An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation |
title_fullStr |
An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation |
title_full_unstemmed |
An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation |
title_sort |
rt pcr-dgge survey of gill-associated bacteria in norwegian seawater-reared atlantic salmon suffering proliferative gill inflammation |
publishDate |
2021 |
url |
https://hdl.handle.net/20.500.12627/69954 https://doi.org/10.1016/j.aquaculture.2009.05.006 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
AQUACULTURE Steinum T. M. , SJASTAD K., FALK K., KVELLESTAD A., COLQUHOUN D. J. , "An RT PCR-DGGE survey of gill-associated bacteria in Norwegian seawater-reared Atlantic salmon suffering proliferative gill inflammation", AQUACULTURE, cilt.293, ss.172-179, 2009 0044-8486 vv_1032021 av_6485d8f0-fa8a-4bcb-b940-032c57eeda58 http://hdl.handle.net/20.500.12627/69954 https://doi.org/10.1016/j.aquaculture.2009.05.006 293 172 179 |
op_doi |
https://doi.org/20.500.12627/6995410.1016/j.aquaculture.2009.05.006 |
container_title |
Aquaculture |
container_volume |
293 |
container_issue |
3-4 |
container_start_page |
172 |
op_container_end_page |
179 |
_version_ |
1802642417255448576 |