Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates
Methanococcoides burtonii is a cold-adapted methanogenic archaeon from Ace Lake in Antarctica. Methanol and methylamines are the only substrates it can use for carbon and energy. We carried out quantitative proteomics using iTRAQ of M. burtonii cells grown on different substrates (methanol in define...
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ftistanbuluniv:oai:http://acikerisim.istanbul.edu.tr:20.500.12627/61388 2023-05-15T13:32:21+02:00 Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates Williams, Timothy J. Cavicchioli, Ricardo Guilhaus, Michael Poljak, Anne Raftery, Mark J. Ertan, Haluk Burg, Dominic W. University of New South Wales Sydney , , 195058 2010 https://hdl.handle.net/20.500.12627/61388 https://doi.org/10.1021/pr9005102 eng eng JOURNAL OF PROTEOME RESEARCH Williams T. J. , Burg D. W. , Ertan H., Raftery M. J. , Poljak A., Guilhaus M., Cavicchioli R., "Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates", JOURNAL OF PROTEOME RESEARCH, cilt.9, sa.2, ss.653-663, 2010 1535-3893 vv_1032021 av_56fe2e35-4f8a-4311-ab86-ae7e8d0b3ae5 http://hdl.handle.net/20.500.12627/61388 https://doi.org/10.1021/pr9005102 9 2 653 663 Yaşam Bilimleri BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ Tıp Temel Bilimler Yaşam Bilimleri (LIFE) Sitogenetik Moleküler Biyoloji ve Genetik Biyoloji ve Biyokimya Biyokimya Temel Tıp Bilimleri Sağlık Bilimleri BİYOKİMYA VE MOLEKÜLER BİYOLOJİ Makale 2010 ftistanbuluniv https://doi.org/20.500.12627/61388 https://doi.org/10.1021/pr9005102 2022-08-10T13:25:20Z Methanococcoides burtonii is a cold-adapted methanogenic archaeon from Ace Lake in Antarctica. Methanol and methylamines are the only substrates it can use for carbon and energy. We carried out quantitative proteomics using iTRAQ of M. burtonii cells grown on different substrates (methanol in defined media or trimethylamine in complex media), using techniques that enriched for secreted and membrane proteins in addition to cytoplasmic proteins. By integrating proteomic data with the complete, manually annotated genome sequence of M. burtonii, we were able to gain new insight into methylotrophic metabolism and the effects of methanol on the cell. Metabolic processing of methanol and methylamines is initiated by methyltransferases specific for each substrate, with multiple paralogs for each of the methyltransferases (similar to other members of the Methanosarcinaceae). In M. burtonii, most methyltransferases appear to have distinct roles in the metabolism of methylated substrates, although two methylamine methyltransferases appear to be nonfunctional. One set of methyltransferases for trimethylamine catabolism appears to be membrane associated, potentially providing a mechanism to directly couple trimethylamine uptake to demethylation. Important roles were highlighted for citrate synthase, glutamine synthetase, acetyl-CoA decarbonylase/synthase, and pyruvate synthase in carbon and nitrogen metabolism during growth on methanol. M. burtonii had only a marginal response to the provision of exogenous amino acids (from yeast extract), indicating that it is predisposed to the endogenous synthesis of amino acids. Growth on methanol appeared to cause oxidative stress in the cell, possibly through the formation of reactive nonoxygen species and formaldehyde, and the oxidative inactivation of corrinoid proteins, with the cell responding by elevating the synthesis of universal stress (Usp) proteins, several nucleic acid binding proteins, and a serpin. In addition, changes in levels of cell envelope proteins were linked to ... Article in Journal/Newspaper Antarc* Antarctica İstanbul Üniversitesi Açık Erişim Sistemi Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Journal of Proteome Research 9 2 653 663 |
institution |
Open Polar |
collection |
İstanbul Üniversitesi Açık Erişim Sistemi |
op_collection_id |
ftistanbuluniv |
language |
English |
topic |
Yaşam Bilimleri BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ Tıp Temel Bilimler Yaşam Bilimleri (LIFE) Sitogenetik Moleküler Biyoloji ve Genetik Biyoloji ve Biyokimya Biyokimya Temel Tıp Bilimleri Sağlık Bilimleri BİYOKİMYA VE MOLEKÜLER BİYOLOJİ |
spellingShingle |
Yaşam Bilimleri BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ Tıp Temel Bilimler Yaşam Bilimleri (LIFE) Sitogenetik Moleküler Biyoloji ve Genetik Biyoloji ve Biyokimya Biyokimya Temel Tıp Bilimleri Sağlık Bilimleri BİYOKİMYA VE MOLEKÜLER BİYOLOJİ Williams, Timothy J. Cavicchioli, Ricardo Guilhaus, Michael Poljak, Anne Raftery, Mark J. Ertan, Haluk Burg, Dominic W. Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates |
topic_facet |
Yaşam Bilimleri BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ Tıp Temel Bilimler Yaşam Bilimleri (LIFE) Sitogenetik Moleküler Biyoloji ve Genetik Biyoloji ve Biyokimya Biyokimya Temel Tıp Bilimleri Sağlık Bilimleri BİYOKİMYA VE MOLEKÜLER BİYOLOJİ |
description |
Methanococcoides burtonii is a cold-adapted methanogenic archaeon from Ace Lake in Antarctica. Methanol and methylamines are the only substrates it can use for carbon and energy. We carried out quantitative proteomics using iTRAQ of M. burtonii cells grown on different substrates (methanol in defined media or trimethylamine in complex media), using techniques that enriched for secreted and membrane proteins in addition to cytoplasmic proteins. By integrating proteomic data with the complete, manually annotated genome sequence of M. burtonii, we were able to gain new insight into methylotrophic metabolism and the effects of methanol on the cell. Metabolic processing of methanol and methylamines is initiated by methyltransferases specific for each substrate, with multiple paralogs for each of the methyltransferases (similar to other members of the Methanosarcinaceae). In M. burtonii, most methyltransferases appear to have distinct roles in the metabolism of methylated substrates, although two methylamine methyltransferases appear to be nonfunctional. One set of methyltransferases for trimethylamine catabolism appears to be membrane associated, potentially providing a mechanism to directly couple trimethylamine uptake to demethylation. Important roles were highlighted for citrate synthase, glutamine synthetase, acetyl-CoA decarbonylase/synthase, and pyruvate synthase in carbon and nitrogen metabolism during growth on methanol. M. burtonii had only a marginal response to the provision of exogenous amino acids (from yeast extract), indicating that it is predisposed to the endogenous synthesis of amino acids. Growth on methanol appeared to cause oxidative stress in the cell, possibly through the formation of reactive nonoxygen species and formaldehyde, and the oxidative inactivation of corrinoid proteins, with the cell responding by elevating the synthesis of universal stress (Usp) proteins, several nucleic acid binding proteins, and a serpin. In addition, changes in levels of cell envelope proteins were linked to ... |
author2 |
University of New South Wales Sydney , , 195058 |
format |
Article in Journal/Newspaper |
author |
Williams, Timothy J. Cavicchioli, Ricardo Guilhaus, Michael Poljak, Anne Raftery, Mark J. Ertan, Haluk Burg, Dominic W. |
author_facet |
Williams, Timothy J. Cavicchioli, Ricardo Guilhaus, Michael Poljak, Anne Raftery, Mark J. Ertan, Haluk Burg, Dominic W. |
author_sort |
Williams, Timothy J. |
title |
Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates |
title_short |
Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates |
title_full |
Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates |
title_fullStr |
Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates |
title_full_unstemmed |
Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates |
title_sort |
global proteomic analysis of the insoluble, soluble, and supernatant fractions of the psychrophilic archaeon methanococcoides burtonii part ii: the effect of different methylated growth substrates |
publishDate |
2010 |
url |
https://hdl.handle.net/20.500.12627/61388 https://doi.org/10.1021/pr9005102 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake |
geographic_facet |
Ace Lake |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
JOURNAL OF PROTEOME RESEARCH Williams T. J. , Burg D. W. , Ertan H., Raftery M. J. , Poljak A., Guilhaus M., Cavicchioli R., "Global Proteomic Analysis of the Insoluble, Soluble, and Supernatant Fractions of the Psychrophilic Archaeon Methanococcoides burtonii Part II: The Effect of Different Methylated Growth Substrates", JOURNAL OF PROTEOME RESEARCH, cilt.9, sa.2, ss.653-663, 2010 1535-3893 vv_1032021 av_56fe2e35-4f8a-4311-ab86-ae7e8d0b3ae5 http://hdl.handle.net/20.500.12627/61388 https://doi.org/10.1021/pr9005102 9 2 653 663 |
op_doi |
https://doi.org/20.500.12627/61388 https://doi.org/10.1021/pr9005102 |
container_title |
Journal of Proteome Research |
container_volume |
9 |
container_issue |
2 |
container_start_page |
653 |
op_container_end_page |
663 |
_version_ |
1766026005668954112 |