Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads
Ostreid herpesvirus-1 (OsHV-1) has been involved in mass mortality episodes of Pacific oysters Crassostrea gigas throughout the world, causing important economic losses to the aquaculture industry. In the present study, magnetic beads (MBs) coated with an anionic polymer were used to capture viable...
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Online Access: | https://hdl.handle.net/20.500.12327/98 https://doi.org/10.1371/journal.pone.0205207 |
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ftirta:oai:repositori.irta.cat:20.500.12327/98 2024-06-23T07:52:16+00:00 Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads Toldrà, Anna Andree, Karl B. Bertomeu, Edgar Roque, Ana Carrasco, Noèlia Gairín, Ignasi Furones, M. Dolores Campàs, Mònica Producció Animal Aigües Marines i Continentals Aqüicultura 2018-10-03 15 application/pdf https://hdl.handle.net/20.500.12327/98 https://doi.org/10.1371/journal.pone.0205207 eng eng Public Library of Science PLoS ONE EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI Toldrà, A., Andree, K., Bertomeu, E., Roque, A., Carrasco, N., & Gairín, I. et al. (2018). Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads. PLOS ONE, 13(10), e0205207. doi:10.1371/journal.pone.0205207 1932-6203 http://hdl.handle.net/20.500.12327/98 https://doi.org/10.1371/journal.pone.0205207 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess 639 info:eu-repo/semantics/article 2018 ftirta https://doi.org/20.500.12327/9810.1371/journal.pone.0205207 2024-06-03T14:07:21Z Ostreid herpesvirus-1 (OsHV-1) has been involved in mass mortality episodes of Pacific oysters Crassostrea gigas throughout the world, causing important economic losses to the aquaculture industry. In the present study, magnetic beads (MBs) coated with an anionic polymer were used to capture viable OsHV-1 from two types of naturally infected matrix: oyster homogenate and seawater. Adsorption of the virus on the MBs and characterisation of the MB-virus conjugates was demonstrated by real-time quantitative PCR (qPCR). To study the infective capacity of the captured virus, MB-virus conjugates were injected in the adductor muscle of naïve spat oysters, using oyster homogenate and seawater without MBs as positive controls, and bare MBs and sterile water as negative controls. Mortalities were induced after injection with MB-virus conjugates and in positive controls, whereas no mortalities were recorded in negative controls. Subsequent OsHV-1 DNA and RNA analysis of the oysters by qPCR and reverse transcription qPCR (RT-qPCR), respectively, confirmed that the virus was the responsible for the mortality event and the ability of the MBs to capture viable viral particles. The capture of viable OsHV-1 using MBs is a rapid and easy isolation method and a promising tool, combined with qPCR, to be applied to OsHV-1 detection in aquaculture facilities. info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Crassostrea gigas Pacific oyster IRTA Pubpro (Institute of Agrifood Research and Technology) Pacific PLOS ONE 13 10 e0205207 |
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Open Polar |
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IRTA Pubpro (Institute of Agrifood Research and Technology) |
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ftirta |
language |
English |
topic |
639 |
spellingShingle |
639 Toldrà, Anna Andree, Karl B. Bertomeu, Edgar Roque, Ana Carrasco, Noèlia Gairín, Ignasi Furones, M. Dolores Campàs, Mònica Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads |
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639 |
description |
Ostreid herpesvirus-1 (OsHV-1) has been involved in mass mortality episodes of Pacific oysters Crassostrea gigas throughout the world, causing important economic losses to the aquaculture industry. In the present study, magnetic beads (MBs) coated with an anionic polymer were used to capture viable OsHV-1 from two types of naturally infected matrix: oyster homogenate and seawater. Adsorption of the virus on the MBs and characterisation of the MB-virus conjugates was demonstrated by real-time quantitative PCR (qPCR). To study the infective capacity of the captured virus, MB-virus conjugates were injected in the adductor muscle of naïve spat oysters, using oyster homogenate and seawater without MBs as positive controls, and bare MBs and sterile water as negative controls. Mortalities were induced after injection with MB-virus conjugates and in positive controls, whereas no mortalities were recorded in negative controls. Subsequent OsHV-1 DNA and RNA analysis of the oysters by qPCR and reverse transcription qPCR (RT-qPCR), respectively, confirmed that the virus was the responsible for the mortality event and the ability of the MBs to capture viable viral particles. The capture of viable OsHV-1 using MBs is a rapid and easy isolation method and a promising tool, combined with qPCR, to be applied to OsHV-1 detection in aquaculture facilities. info:eu-repo/semantics/publishedVersion |
author2 |
Producció Animal Aigües Marines i Continentals Aqüicultura |
format |
Article in Journal/Newspaper |
author |
Toldrà, Anna Andree, Karl B. Bertomeu, Edgar Roque, Ana Carrasco, Noèlia Gairín, Ignasi Furones, M. Dolores Campàs, Mònica |
author_facet |
Toldrà, Anna Andree, Karl B. Bertomeu, Edgar Roque, Ana Carrasco, Noèlia Gairín, Ignasi Furones, M. Dolores Campàs, Mònica |
author_sort |
Toldrà, Anna |
title |
Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads |
title_short |
Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads |
title_full |
Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads |
title_fullStr |
Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads |
title_full_unstemmed |
Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads |
title_sort |
rapid capture and detection of ostreid herpesvirus-1 from pacific oyster crassostrea gigas and seawater using magnetic beads |
publisher |
Public Library of Science |
publishDate |
2018 |
url |
https://hdl.handle.net/20.500.12327/98 https://doi.org/10.1371/journal.pone.0205207 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
PLoS ONE EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI Toldrà, A., Andree, K., Bertomeu, E., Roque, A., Carrasco, N., & Gairín, I. et al. (2018). Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads. PLOS ONE, 13(10), e0205207. doi:10.1371/journal.pone.0205207 1932-6203 http://hdl.handle.net/20.500.12327/98 https://doi.org/10.1371/journal.pone.0205207 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.12327/9810.1371/journal.pone.0205207 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
10 |
container_start_page |
e0205207 |
_version_ |
1802643517256761344 |