A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish
Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric...
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ftirta:oai:repositori.irta.cat:20.500.12327/744 2023-07-02T03:32:03+02:00 A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish Campàs, Mònica Reverté, Jaume Rambla-Alegre, Maria Campbell, Katrina Gerssen, Arjen Diogène, Jorge Producció Animal Aigües Marines i Continentals 2020-04-05 16 application/pdf https://hdl.handle.net/20.500.12327/744 https://doi.org/10.1016/j.fct.2020.111315 eng eng Elsevier Food and Chemical Toxicology EC/H2020/773400/EU/Nutritious, safe and sustainable seafood for consumers of tomorrow/SEAFOODTOMORROW Campàs, Mònica, Jaume Reverté, Maria Rambla-Alegre, Katrina Campbell, Arjen Gerssen, and Jorge Diogène. 2020. "A Fast Magnetic Bead-Based Colorimetric Immunoassay For The Detection Of Tetrodotoxins In Shellfish". Food And Chemical Toxicology 140: 111315. Elsevier BV. doi:10.1016/j.fct.2020.111315. 0278-6915 http://hdl.handle.net/20.500.12327/744 https://doi.org/10.1016/j.fct.2020.111315 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess 574 info:eu-repo/semantics/article 2020 ftirta https://doi.org/20.500.12327/74410.1016/j.fct.2020.111315 2023-06-13T23:20:15Z Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers. info:eu-repo/semantics/acceptedVersion Article in Journal/Newspaper Crassostrea gigas IRTA Pubpro (Institute of Agrifood Research and Technology) Pacific Food and Chemical Toxicology 140 111315 |
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English |
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574 Campàs, Mònica Reverté, Jaume Rambla-Alegre, Maria Campbell, Katrina Gerssen, Arjen Diogène, Jorge A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
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574 |
description |
Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers. info:eu-repo/semantics/acceptedVersion |
author2 |
Producció Animal Aigües Marines i Continentals |
format |
Article in Journal/Newspaper |
author |
Campàs, Mònica Reverté, Jaume Rambla-Alegre, Maria Campbell, Katrina Gerssen, Arjen Diogène, Jorge |
author_facet |
Campàs, Mònica Reverté, Jaume Rambla-Alegre, Maria Campbell, Katrina Gerssen, Arjen Diogène, Jorge |
author_sort |
Campàs, Mònica |
title |
A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
title_short |
A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
title_full |
A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
title_fullStr |
A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
title_full_unstemmed |
A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
title_sort |
fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://hdl.handle.net/20.500.12327/744 https://doi.org/10.1016/j.fct.2020.111315 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Food and Chemical Toxicology EC/H2020/773400/EU/Nutritious, safe and sustainable seafood for consumers of tomorrow/SEAFOODTOMORROW Campàs, Mònica, Jaume Reverté, Maria Rambla-Alegre, Katrina Campbell, Arjen Gerssen, and Jorge Diogène. 2020. "A Fast Magnetic Bead-Based Colorimetric Immunoassay For The Detection Of Tetrodotoxins In Shellfish". Food And Chemical Toxicology 140: 111315. Elsevier BV. doi:10.1016/j.fct.2020.111315. 0278-6915 http://hdl.handle.net/20.500.12327/744 https://doi.org/10.1016/j.fct.2020.111315 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.12327/74410.1016/j.fct.2020.111315 |
container_title |
Food and Chemical Toxicology |
container_volume |
140 |
container_start_page |
111315 |
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1770271514170490880 |