Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico)
Triploid Crassostrea gigas were cultured during 13 months in Nestier-type oyster trays. The impact of environmental parameters on the physiological and immunological parameters was evaluated. Temperature, salinity and seston were recorded monthly. Seventeen oysters were sampled monthly for immunolog...
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ftipnmexicoir:oai:www.repositoriodigital.ipn.mx:123456789/8307 2023-07-16T03:58:03+02:00 Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) Luna González, Antonio Romero Geraldo, María de Jesús Campa Córdova, Angel Orduña Rojas, Javier Valles Jiménez, Rubén Ruiz Verdugo, César A. 2012-11-21 http://www.repositoriodigital.ipn.mx/handle/123456789/8307 en eng http://www.repositoriodigital.ipn.mx/handle/123456789/8307 Article 2012 ftipnmexicoir 2023-06-28T10:37:37Z Triploid Crassostrea gigas were cultured during 13 months in Nestier-type oyster trays. The impact of environmental parameters on the physiological and immunological parameters was evaluated. Temperature, salinity and seston were recorded monthly. Seventeen oysters were sampled monthly for immunological and condition index (CI) analyses. Samples were obtained as a haemolymph lysate supernatant (HLS). Protein content was determined using the Bradford method. The activity of hydrolytic enzymes was determined using the API ZYM kit and the lysoplate assay. Seston showed di¡erent patterns throughout the cycle. Condition index showed a positive correlation with the protein content of HLS. Protein showed a negative correlationwith temperature. Eleven hydrolytic enzymes were detected in samples and higher enzymatic activity corresponded to leucine arylamidase and esterase. Leucine arylamidase and lysozyme activity showed a positive correlationwith temperature. Oyster mortality was 28% in our modules and 70% in the oyster farm. Oysters showed low values of CI and haemolymph protein content in summer^autumn when mortalities were observed in the culture system. This ¢nding suggests that these stressed oysters may have insu⁄cient energy to invest intheir immune system. It appears that oystermortality inthe culture systemresulted from a combination of animal overcrowding, high temperatura and low salinity. Investigación 1 pag. Article in Journal/Newspaper Crassostrea gigas Pacific oyster El Instituto Politécnico Nacional (IPN): Repositorio Digital Institucional (RDI) Pacific |
institution |
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collection |
El Instituto Politécnico Nacional (IPN): Repositorio Digital Institucional (RDI) |
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ftipnmexicoir |
language |
English |
description |
Triploid Crassostrea gigas were cultured during 13 months in Nestier-type oyster trays. The impact of environmental parameters on the physiological and immunological parameters was evaluated. Temperature, salinity and seston were recorded monthly. Seventeen oysters were sampled monthly for immunological and condition index (CI) analyses. Samples were obtained as a haemolymph lysate supernatant (HLS). Protein content was determined using the Bradford method. The activity of hydrolytic enzymes was determined using the API ZYM kit and the lysoplate assay. Seston showed di¡erent patterns throughout the cycle. Condition index showed a positive correlation with the protein content of HLS. Protein showed a negative correlationwith temperature. Eleven hydrolytic enzymes were detected in samples and higher enzymatic activity corresponded to leucine arylamidase and esterase. Leucine arylamidase and lysozyme activity showed a positive correlationwith temperature. Oyster mortality was 28% in our modules and 70% in the oyster farm. Oysters showed low values of CI and haemolymph protein content in summer^autumn when mortalities were observed in the culture system. This ¢nding suggests that these stressed oysters may have insu⁄cient energy to invest intheir immune system. It appears that oystermortality inthe culture systemresulted from a combination of animal overcrowding, high temperatura and low salinity. Investigación 1 pag. |
format |
Article in Journal/Newspaper |
author |
Luna González, Antonio Romero Geraldo, María de Jesús Campa Córdova, Angel Orduña Rojas, Javier Valles Jiménez, Rubén Ruiz Verdugo, César A. |
spellingShingle |
Luna González, Antonio Romero Geraldo, María de Jesús Campa Córdova, Angel Orduña Rojas, Javier Valles Jiménez, Rubén Ruiz Verdugo, César A. Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) |
author_facet |
Luna González, Antonio Romero Geraldo, María de Jesús Campa Córdova, Angel Orduña Rojas, Javier Valles Jiménez, Rubén Ruiz Verdugo, César A. |
author_sort |
Luna González, Antonio |
title |
Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) |
title_short |
Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) |
title_full |
Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) |
title_fullStr |
Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) |
title_full_unstemmed |
Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa, Mexico) |
title_sort |
seasonal variations in the immunological and physiological parameters of the pacific oyster crassostrea gigas cultured in bahía de macapule (sinaloa, mexico) |
publishDate |
2012 |
url |
http://www.repositoriodigital.ipn.mx/handle/123456789/8307 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.repositoriodigital.ipn.mx/handle/123456789/8307 |
_version_ |
1771545031622000640 |