Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment
Photodynamic therapy (PDT) promotes skin improvement according to many practitioners, however the immediately in vivo assessment of its response remains clinically inaccessible. As a non-invasive modality, optical coherence tomography (OCT) has been shown a feasible optical diagnostic technique that...
Published in: | SPIE Proceedings, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII |
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Online Access: | http://repositorio.ipen.br/handle/123456789/27653 https://doi.org/10.1117/12.2040443 |
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ftipen:oai:repositorio.ipen.br:123456789/27653 2023-11-12T04:16:50+01:00 Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment JORGE, ANA E.S. CAMPOS, CAROLINA P. FREITAS, ANDERSON Z. de BAGNATO, VANDERLEI S. OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th IZATT, JOSEPH A. FUJIMOTO, JAMES G. TUCHIN, VALERY V. I February 01, 2014 89343N-1 - 89343-3 http://repositorio.ipen.br/handle/123456789/27653 https://doi.org/10.1117/12.2040443 unknown International Society for Optics and Photonics SPIE Proceedings Series, 8934 http://repositorio.ipen.br/handle/123456789/27653 doi:10.1117/12.2040443 openAccess tomography optical systems coherence length mice skin thickness aminolevulinic acid epidermis carcinomas in vivo animal tissues Texto completo de evento 2014 ftipen https://doi.org/10.1117/12.2040443 2023-10-30T16:27:40Z Photodynamic therapy (PDT) promotes skin improvement according to many practitioners, however the immediately in vivo assessment of its response remains clinically inaccessible. As a non-invasive modality, optical coherence tomography (OCT) has been shown a feasible optical diagnostic technique that provides images in real time, avoiding tissue biopsies. For this reason, our investigation focused on evaluates the PDT effect on a rodent model by means of OCT. Therefore, a normal hairless mouse skin has undergone a single-session PDT, which was performed with topical 5- aminolevulinic acid (ALA) cream using a red (630 nm) light emitting diode (LED) which reached the light dose of 75 J/cm2. As the optical imaging tool, an OCT (930 nm) with axial resolution of 6.0 microns in air was used, generating images with contact to the mouse skin before, immediately after, 24 hours, and 2 weeks after the correspondent procedure. Our result demonstrates that, within 24 hours after ALA-PDT, the mouse skin from the PDT group has shown epidermal thickness (ET), which has substantially increased after 2 weeks from the treatment day. Moreover, the skin surface has become evener after ALA-PDT. Concluding, this investigation demonstrates that the OCT is a feasible and reliable technique that allows real-time cross-sectional imaging of skin, which can quantify an outcome and predict whether the PDT reaches its goal. Text evener Repositório Digital do IPEN (Instituto de Pesquisas Energéticas e Nucleares) SPIE Proceedings, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII 8934 89343N |
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Repositório Digital do IPEN (Instituto de Pesquisas Energéticas e Nucleares) |
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ftipen |
language |
unknown |
topic |
tomography optical systems coherence length mice skin thickness aminolevulinic acid epidermis carcinomas in vivo animal tissues |
spellingShingle |
tomography optical systems coherence length mice skin thickness aminolevulinic acid epidermis carcinomas in vivo animal tissues JORGE, ANA E.S. CAMPOS, CAROLINA P. FREITAS, ANDERSON Z. de BAGNATO, VANDERLEI S. OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
topic_facet |
tomography optical systems coherence length mice skin thickness aminolevulinic acid epidermis carcinomas in vivo animal tissues |
description |
Photodynamic therapy (PDT) promotes skin improvement according to many practitioners, however the immediately in vivo assessment of its response remains clinically inaccessible. As a non-invasive modality, optical coherence tomography (OCT) has been shown a feasible optical diagnostic technique that provides images in real time, avoiding tissue biopsies. For this reason, our investigation focused on evaluates the PDT effect on a rodent model by means of OCT. Therefore, a normal hairless mouse skin has undergone a single-session PDT, which was performed with topical 5- aminolevulinic acid (ALA) cream using a red (630 nm) light emitting diode (LED) which reached the light dose of 75 J/cm2. As the optical imaging tool, an OCT (930 nm) with axial resolution of 6.0 microns in air was used, generating images with contact to the mouse skin before, immediately after, 24 hours, and 2 weeks after the correspondent procedure. Our result demonstrates that, within 24 hours after ALA-PDT, the mouse skin from the PDT group has shown epidermal thickness (ET), which has substantially increased after 2 weeks from the treatment day. Moreover, the skin surface has become evener after ALA-PDT. Concluding, this investigation demonstrates that the OCT is a feasible and reliable technique that allows real-time cross-sectional imaging of skin, which can quantify an outcome and predict whether the PDT reaches its goal. |
author2 |
IZATT, JOSEPH A. FUJIMOTO, JAMES G. TUCHIN, VALERY V. |
format |
Text |
author |
JORGE, ANA E.S. CAMPOS, CAROLINA P. FREITAS, ANDERSON Z. de BAGNATO, VANDERLEI S. OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th |
author_facet |
JORGE, ANA E.S. CAMPOS, CAROLINA P. FREITAS, ANDERSON Z. de BAGNATO, VANDERLEI S. OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th |
author_sort |
JORGE, ANA E.S. |
title |
Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
title_short |
Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
title_full |
Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
title_fullStr |
Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
title_full_unstemmed |
Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
title_sort |
photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment |
publisher |
International Society for Optics and Photonics |
publishDate |
2014 |
url |
http://repositorio.ipen.br/handle/123456789/27653 https://doi.org/10.1117/12.2040443 |
op_coverage |
I |
genre |
evener |
genre_facet |
evener |
op_relation |
SPIE Proceedings Series, 8934 http://repositorio.ipen.br/handle/123456789/27653 doi:10.1117/12.2040443 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1117/12.2040443 |
container_title |
SPIE Proceedings, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII |
container_volume |
8934 |
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89343N |
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1782333894286114816 |