Maximization of fructose esters synthesis by response surface methodology

• Published in: New Biotechnology
• Main Authors: Neta, Nair S., Peres, António M., Teixeira, José, Rodrigues, Lígia R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2011
• Subjects: Candida antartica B; Fructose esters; Response surface methodology; antartic*
• Online Access: http://hdl.handle.net/10198/6406; https://doi.org/10.1016/j.nbt.2011.02.007

Enzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions. The authors acknowledge the AlBan Programme (European Union Programme of High Level Scholarships for Latin America) for financial support of N. Lopes through the research grant E07D401544BR