Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods
Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cult...
Published in: | Frontiers in Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | http://hdl.handle.net/10198/20099 https://doi.org/10.3389/fmicb.2019.01018 |
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ftipb:oai:bibliotecadigital.ipb.pt:10198/20099 2023-07-02T03:29:45+02:00 Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods Rego, Adriana Raio, Francisco Martins, Teresa P. Ribeiro, Hugo Sousa, António G.G. Séneca, Joana Baptista, Mafalda S. Lee, Charles K. Craig Cary, S. Ramos, Vitor Carvalho, Maria F. Leão, Pedro N. Magalhães, Catarina 2019 http://hdl.handle.net/10198/20099 https://doi.org/10.3389/fmicb.2019.01018 eng eng IF/01358/2014 Rego, Adriana; Raio, Francisco; Martins, Teresa P.; Ribeiro, Hugo; Sousa, António G.G.; Séneca, Joana; Baptista, Mafalda S.; Lee, Charles K.; Craig Cary, S.; Ramos, Vitor; Carvalho, Maria F.; Leão, Pedro N.; Magalhães, Catarina (2019). Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods. Frontiers in Microbiology. ISSN -. 10, p. http://hdl.handle.net/10198/20099 doi:10.3389/fmicb.2019.01018 openAccess Actinobacteria Antarctic microenvironments Antarctic soil Bacteria diversity Bacterial cultivability Cyanobacteria Endolitic microbiota article 2019 ftipb https://doi.org/10.3389/fmicb.2019.01018 2023-06-13T18:31:24Z Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria and cyanobacteria diversity along different soil and endolithic micro-environments of Victoria Valley in McMurdo Dry Valleys. Our results demonstrate that the Dry Valleys actinobacteria and cyanobacteria distribution is driven by environmental forces, in particular the effect of water availability and endolithic environments clearly conditioned the distribution of those communities. Data derived from HTS show that the percentage of cyanobacteria decreases from about 20% in the sample closest to the water source to negligible values on the last three samples of the transect with less water availability. Inversely, actinobacteria relative abundance increases from about 20% in wet soils to over 50% in the driest samples. Over 30% of the total HTS data set was composed of actinobacterial strains, mainly distributed by 5 families: Sporichthyaceae, Euzebyaceae, Patulibacteraceae, Nocardioidaceae, and Rubrobacteraceae. However, the 11 actinobacterial strains isolated in this study, belonged to Micrococcaceae and Dermacoccaceae families that were underrepresented in the HTS data set. A total of 10 cyanobacterial strains from the order Synechococcales were also isolated, distributed by 4 different genera (Nodosilinea, Leptolyngbya, Pectolyngbya, and Acaryochloris-like). In agreement with the cultivation results, Leptolyngbya was identified as dominant genus in the HTS data set. Acaryochloris-like cyanobacteria were found exclusively in the endolithic sample and represented 44% of the total 16S rRNA sequences, although despite our efforts we were not able to properly isolate any strain from this Acaryochloris-related group. The importance of combining cultivation and ... Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Biblioteca Digital do Instituto Politécnico de Bragança (IPB) Antarctic McMurdo Dry Valleys Victoria Valley ENVELOPE(162.000,162.000,-77.383,-77.383) Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
Biblioteca Digital do Instituto Politécnico de Bragança (IPB) |
op_collection_id |
ftipb |
language |
English |
topic |
Actinobacteria Antarctic microenvironments Antarctic soil Bacteria diversity Bacterial cultivability Cyanobacteria Endolitic microbiota |
spellingShingle |
Actinobacteria Antarctic microenvironments Antarctic soil Bacteria diversity Bacterial cultivability Cyanobacteria Endolitic microbiota Rego, Adriana Raio, Francisco Martins, Teresa P. Ribeiro, Hugo Sousa, António G.G. Séneca, Joana Baptista, Mafalda S. Lee, Charles K. Craig Cary, S. Ramos, Vitor Carvalho, Maria F. Leão, Pedro N. Magalhães, Catarina Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods |
topic_facet |
Actinobacteria Antarctic microenvironments Antarctic soil Bacteria diversity Bacterial cultivability Cyanobacteria Endolitic microbiota |
description |
Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria and cyanobacteria diversity along different soil and endolithic micro-environments of Victoria Valley in McMurdo Dry Valleys. Our results demonstrate that the Dry Valleys actinobacteria and cyanobacteria distribution is driven by environmental forces, in particular the effect of water availability and endolithic environments clearly conditioned the distribution of those communities. Data derived from HTS show that the percentage of cyanobacteria decreases from about 20% in the sample closest to the water source to negligible values on the last three samples of the transect with less water availability. Inversely, actinobacteria relative abundance increases from about 20% in wet soils to over 50% in the driest samples. Over 30% of the total HTS data set was composed of actinobacterial strains, mainly distributed by 5 families: Sporichthyaceae, Euzebyaceae, Patulibacteraceae, Nocardioidaceae, and Rubrobacteraceae. However, the 11 actinobacterial strains isolated in this study, belonged to Micrococcaceae and Dermacoccaceae families that were underrepresented in the HTS data set. A total of 10 cyanobacterial strains from the order Synechococcales were also isolated, distributed by 4 different genera (Nodosilinea, Leptolyngbya, Pectolyngbya, and Acaryochloris-like). In agreement with the cultivation results, Leptolyngbya was identified as dominant genus in the HTS data set. Acaryochloris-like cyanobacteria were found exclusively in the endolithic sample and represented 44% of the total 16S rRNA sequences, although despite our efforts we were not able to properly isolate any strain from this Acaryochloris-related group. The importance of combining cultivation and ... |
format |
Article in Journal/Newspaper |
author |
Rego, Adriana Raio, Francisco Martins, Teresa P. Ribeiro, Hugo Sousa, António G.G. Séneca, Joana Baptista, Mafalda S. Lee, Charles K. Craig Cary, S. Ramos, Vitor Carvalho, Maria F. Leão, Pedro N. Magalhães, Catarina |
author_facet |
Rego, Adriana Raio, Francisco Martins, Teresa P. Ribeiro, Hugo Sousa, António G.G. Séneca, Joana Baptista, Mafalda S. Lee, Charles K. Craig Cary, S. Ramos, Vitor Carvalho, Maria F. Leão, Pedro N. Magalhães, Catarina |
author_sort |
Rego, Adriana |
title |
Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods |
title_short |
Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods |
title_full |
Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods |
title_fullStr |
Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods |
title_full_unstemmed |
Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods |
title_sort |
actinobacteria and cyanobacteria diversity in terrestrial antarctic microenvironments evaluated by culture-dependent and independent methods |
publishDate |
2019 |
url |
http://hdl.handle.net/10198/20099 https://doi.org/10.3389/fmicb.2019.01018 |
long_lat |
ENVELOPE(162.000,162.000,-77.383,-77.383) |
geographic |
Antarctic McMurdo Dry Valleys Victoria Valley |
geographic_facet |
Antarctic McMurdo Dry Valleys Victoria Valley |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_relation |
IF/01358/2014 Rego, Adriana; Raio, Francisco; Martins, Teresa P.; Ribeiro, Hugo; Sousa, António G.G.; Séneca, Joana; Baptista, Mafalda S.; Lee, Charles K.; Craig Cary, S.; Ramos, Vitor; Carvalho, Maria F.; Leão, Pedro N.; Magalhães, Catarina (2019). Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments evaluated by culture-dependent and independent methods. Frontiers in Microbiology. ISSN -. 10, p. http://hdl.handle.net/10198/20099 doi:10.3389/fmicb.2019.01018 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2019.01018 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
_version_ |
1770272374397075456 |