Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing
Studies of innate immunity in fish species of special concern are essential for better understanding of their health status during hatchery rearing conditions. The cytochemical and morphological characterizations of blood granulocytes have been used to provide information about phylogenetic differen...
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ftiowastateuniv:oai:lib.dr.iastate.edu:vpath_pubs-1074 2023-05-15T14:31:22+02:00 Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing Palić, Dušan Beck, Linda S. Palić, Jelena Andreasen, Claire B. 2011-02-01T08:00:00Z application/pdf https://lib.dr.iastate.edu/vpath_pubs/71 https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1074&context=vpath_pubs en eng Iowa State University Digital Repository https://lib.dr.iastate.edu/vpath_pubs/71 https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1074&context=vpath_pubs Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Veterinary Pathology Publications and Papers Fish species of special concern leukocyte morphology cytochemical characterization granulocyte function Aquaculture and Fisheries Veterinary Pathology and Pathobiology text 2011 ftiowastateuniv 2018-11-25T23:00:29Z Studies of innate immunity in fish species of special concern are essential for better understanding of their health status during hatchery rearing conditions. The cytochemical and morphological characterizations of blood granulocytes have been used to provide information about phylogenetic differences and determine the potential use of neutrophil function assays. Rapid, simple, cytochemical staining kits used routinely for staining mammalian granulocytes have been used to characterize granulocytes from blood of four fish species: Arctic grayling, cutthroat trout, June sucker, and shovelnose sturgeon. Blood smears were stained with Peroxidase 391 (myeloperoxidase, MPO), alkaline phosphatase (AP), Periodic Acid Schiff (PAS) and Diff-quick stain; examined using bright field and differential interference contrast microscopy. Granulocytes on blood smears were evaluated based on the cell morphology, and presence or absence of the specific chromogen. Presence of lymphocytes, monocytes, platelets/thrombocytes and granulocytes was determined in all fish species. Arctic grayling, June sucker, and cutthroat trout had MPO positive granulocytes, while shovelnose sturgeon heterophils had positive reaction for leukocyte AP, but not MPO. Presence of MPO indicated potential to measure oxidative burst and degranulation of neutrophil primary granules in Arctic grayling, cutthroat trout and June sucker. Absence of MPO in shovelnose sturgeon suggested use of different enzyme marker (AP) in degranulation assay for this species. Standardization of cytochemical techniques allowed forrapid screening of leukocyte types, reducing the number of fish, time and effort to select adequate neutrophil function assays to be used in studies of health status in species of special concern. Text Arctic grayling Arctic Digital Repository @ Iowa State University Arctic |
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Digital Repository @ Iowa State University |
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ftiowastateuniv |
language |
English |
topic |
Fish species of special concern leukocyte morphology cytochemical characterization granulocyte function Aquaculture and Fisheries Veterinary Pathology and Pathobiology |
spellingShingle |
Fish species of special concern leukocyte morphology cytochemical characterization granulocyte function Aquaculture and Fisheries Veterinary Pathology and Pathobiology Palić, Dušan Beck, Linda S. Palić, Jelena Andreasen, Claire B. Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing |
topic_facet |
Fish species of special concern leukocyte morphology cytochemical characterization granulocyte function Aquaculture and Fisheries Veterinary Pathology and Pathobiology |
description |
Studies of innate immunity in fish species of special concern are essential for better understanding of their health status during hatchery rearing conditions. The cytochemical and morphological characterizations of blood granulocytes have been used to provide information about phylogenetic differences and determine the potential use of neutrophil function assays. Rapid, simple, cytochemical staining kits used routinely for staining mammalian granulocytes have been used to characterize granulocytes from blood of four fish species: Arctic grayling, cutthroat trout, June sucker, and shovelnose sturgeon. Blood smears were stained with Peroxidase 391 (myeloperoxidase, MPO), alkaline phosphatase (AP), Periodic Acid Schiff (PAS) and Diff-quick stain; examined using bright field and differential interference contrast microscopy. Granulocytes on blood smears were evaluated based on the cell morphology, and presence or absence of the specific chromogen. Presence of lymphocytes, monocytes, platelets/thrombocytes and granulocytes was determined in all fish species. Arctic grayling, June sucker, and cutthroat trout had MPO positive granulocytes, while shovelnose sturgeon heterophils had positive reaction for leukocyte AP, but not MPO. Presence of MPO indicated potential to measure oxidative burst and degranulation of neutrophil primary granules in Arctic grayling, cutthroat trout and June sucker. Absence of MPO in shovelnose sturgeon suggested use of different enzyme marker (AP) in degranulation assay for this species. Standardization of cytochemical techniques allowed forrapid screening of leukocyte types, reducing the number of fish, time and effort to select adequate neutrophil function assays to be used in studies of health status in species of special concern. |
format |
Text |
author |
Palić, Dušan Beck, Linda S. Palić, Jelena Andreasen, Claire B. |
author_facet |
Palić, Dušan Beck, Linda S. Palić, Jelena Andreasen, Claire B. |
author_sort |
Palić, Dušan |
title |
Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing |
title_short |
Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing |
title_full |
Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing |
title_fullStr |
Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing |
title_full_unstemmed |
Use of Rapid Cytochemical Staining to Characterize fish blood granulocytes in Species of Special Concern and Determine Potential for Function Testing |
title_sort |
use of rapid cytochemical staining to characterize fish blood granulocytes in species of special concern and determine potential for function testing |
publisher |
Iowa State University Digital Repository |
publishDate |
2011 |
url |
https://lib.dr.iastate.edu/vpath_pubs/71 https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1074&context=vpath_pubs |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic grayling Arctic |
genre_facet |
Arctic grayling Arctic |
op_source |
Veterinary Pathology Publications and Papers |
op_relation |
https://lib.dr.iastate.edu/vpath_pubs/71 https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1074&context=vpath_pubs |
op_rights |
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. |
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