Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes
Active-targeting nanomedicine formulations have an intricate in vivo behavior. Nanomedicines developed to target endothelial αvβ3-integrin are recently demonstrated to display extensive uptake by circulating phagocytes. These phagocytes show inherent tumor-homing capacities and therefore are capable...
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Online Access: | https://hdl.handle.net/20.500.12105/14210 https://doi.org/10.1002/advs.202100370 |
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ftintsaludcarlos:oai:repisalud.isciii.es:20.500.12105/14210 2024-06-23T07:57:14+00:00 Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes Sofias, Alexandros Marios Bjørkøy, Geir Ochando, Jordi Sønstevold, Linda Hegvik, Maria Davies, Catharina de Lange Haraldseth, Olav Lammers, Twan Mulder, Willem J M Hak, Sjoerd Mulder, Willem JM Tromsø Research Foundation Trond Mohn Foundation Norwegian Research Centre Central Norway Regional Health Authority 2021 https://hdl.handle.net/20.500.12105/14210 https://doi.org/10.1002/advs.202100370 eng eng Wiley https://doi.org/10.1002/advs.202100370 Adv. Sci.2021;8 :2100370 2198-3844 http://hdl.handle.net/20.500.12105/14210 doi:10.1002/advs.202100370 Advanced Science http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional open access Arginine Glycine Aspartate Immunotherapy Inflammation Intravital microscopy Nanomedicines Neutrophils Phagocyte hitchhiking journal article VoR 2021 ftintsaludcarlos https://doi.org/20.500.12105/1421010.1002/advs.202100370 2024-05-27T23:35:06Z Active-targeting nanomedicine formulations have an intricate in vivo behavior. Nanomedicines developed to target endothelial αvβ3-integrin are recently demonstrated to display extensive uptake by circulating phagocytes. These phagocytes show inherent tumor-homing capacities and therefore are capable of actively delivering the endocytosed nanomaterial in lesions. Here, the targeting kinetics and mechanisms of cyclic arginine–glycine–aspartate (cRGD)-decorated lipid nanoparticles (NPs) toward activated vasculature in inflamed lesions during wound healing are studied. The cRGD-NP targeting toward inflamed lesions is identified to be mechanistically similar to the NP accumulation in cancerous lesions. Through a complementary experimental approach, it is observed that circulating phagocytes engage cRGD-NPs and are subsequently homed to the inflamed endothelium. The inflammation-associated phagocytes remain static among endothelial cells upon targeting, resulting in the extensive presence of cRGD-NP-positive phagocytes in the angiogenic vessels. Hence, phagocytic immune cells contribute to cRGD-NP targeting toward angiogenesis. This mechanistic study underlines the need for detailed investigations of NP in vivo behavior. This is critically important for the realization of NPs potential as advanced (immunological) therapeutic agents. This work was supported by the Central Norway Regional Health Authority “Helse Midt-Norge” (A.M.S.: Ph.D. stipend [90062100] and travel grant [90284100]; S.H.: researcher grant [90262100]), the Norwegian Research Council (S.H.: 230788/F20), the Tromsø Research Foundation, and Trond Mohn Foundation (S.H.: 180°N project). Sí Article in Journal/Newspaper Tromsø REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) Norway Tromsø Advanced Science 8 13 2100370 |
institution |
Open Polar |
collection |
REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
op_collection_id |
ftintsaludcarlos |
language |
English |
topic |
Arginine Glycine Aspartate Immunotherapy Inflammation Intravital microscopy Nanomedicines Neutrophils Phagocyte hitchhiking |
spellingShingle |
Arginine Glycine Aspartate Immunotherapy Inflammation Intravital microscopy Nanomedicines Neutrophils Phagocyte hitchhiking Sofias, Alexandros Marios Bjørkøy, Geir Ochando, Jordi Sønstevold, Linda Hegvik, Maria Davies, Catharina de Lange Haraldseth, Olav Lammers, Twan Mulder, Willem J M Hak, Sjoerd Mulder, Willem JM Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes |
topic_facet |
Arginine Glycine Aspartate Immunotherapy Inflammation Intravital microscopy Nanomedicines Neutrophils Phagocyte hitchhiking |
description |
Active-targeting nanomedicine formulations have an intricate in vivo behavior. Nanomedicines developed to target endothelial αvβ3-integrin are recently demonstrated to display extensive uptake by circulating phagocytes. These phagocytes show inherent tumor-homing capacities and therefore are capable of actively delivering the endocytosed nanomaterial in lesions. Here, the targeting kinetics and mechanisms of cyclic arginine–glycine–aspartate (cRGD)-decorated lipid nanoparticles (NPs) toward activated vasculature in inflamed lesions during wound healing are studied. The cRGD-NP targeting toward inflamed lesions is identified to be mechanistically similar to the NP accumulation in cancerous lesions. Through a complementary experimental approach, it is observed that circulating phagocytes engage cRGD-NPs and are subsequently homed to the inflamed endothelium. The inflammation-associated phagocytes remain static among endothelial cells upon targeting, resulting in the extensive presence of cRGD-NP-positive phagocytes in the angiogenic vessels. Hence, phagocytic immune cells contribute to cRGD-NP targeting toward angiogenesis. This mechanistic study underlines the need for detailed investigations of NP in vivo behavior. This is critically important for the realization of NPs potential as advanced (immunological) therapeutic agents. This work was supported by the Central Norway Regional Health Authority “Helse Midt-Norge” (A.M.S.: Ph.D. stipend [90062100] and travel grant [90284100]; S.H.: researcher grant [90262100]), the Norwegian Research Council (S.H.: 230788/F20), the Tromsø Research Foundation, and Trond Mohn Foundation (S.H.: 180°N project). Sí |
author2 |
Tromsø Research Foundation Trond Mohn Foundation Norwegian Research Centre Central Norway Regional Health Authority |
format |
Article in Journal/Newspaper |
author |
Sofias, Alexandros Marios Bjørkøy, Geir Ochando, Jordi Sønstevold, Linda Hegvik, Maria Davies, Catharina de Lange Haraldseth, Olav Lammers, Twan Mulder, Willem J M Hak, Sjoerd Mulder, Willem JM |
author_facet |
Sofias, Alexandros Marios Bjørkøy, Geir Ochando, Jordi Sønstevold, Linda Hegvik, Maria Davies, Catharina de Lange Haraldseth, Olav Lammers, Twan Mulder, Willem J M Hak, Sjoerd Mulder, Willem JM |
author_sort |
Sofias, Alexandros Marios |
title |
Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes |
title_short |
Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes |
title_full |
Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes |
title_fullStr |
Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes |
title_full_unstemmed |
Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes |
title_sort |
cyclic arginine–glycine–aspartate‐decorated lipid nanoparticle targeting toward inflammatory lesions involves hitchhiking with phagocytes |
publisher |
Wiley |
publishDate |
2021 |
url |
https://hdl.handle.net/20.500.12105/14210 https://doi.org/10.1002/advs.202100370 |
geographic |
Norway Tromsø |
geographic_facet |
Norway Tromsø |
genre |
Tromsø |
genre_facet |
Tromsø |
op_relation |
https://doi.org/10.1002/advs.202100370 Adv. Sci.2021;8 :2100370 2198-3844 http://hdl.handle.net/20.500.12105/14210 doi:10.1002/advs.202100370 Advanced Science |
op_rights |
http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional open access |
op_doi |
https://doi.org/20.500.12105/1421010.1002/advs.202100370 |
container_title |
Advanced Science |
container_volume |
8 |
container_issue |
13 |
container_start_page |
2100370 |
_version_ |
1802650780397731840 |