From Targeted Quantification to Untargeted Metabolomics

Metabolomics is an emerging and rapidly evolving technology tool, which involves quantitative and qualitative metabolite assessments science. It offers tremendous promise for different applications in various fields such as medical, environmental, nutrition, and agricultural sciences. Metabolomic ap...

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Main Authors: Lelli, Veronica, Belardo, Antonio, Timperio, Anna Maria
Format: Other/Unknown Material
Language:English
Published: IntechOpen 2021
Subjects:
Online Access:https://mts.intechopen.com/articles/show/title/from-targeted-quantification-to-untargeted-metabolomics
https://doi.org/10.5772/intechopen.96852
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spelling ftintech:oai:intechopen.com:75755 2023-05-15T13:56:47+02:00 From Targeted Quantification to Untargeted Metabolomics Lelli, Veronica Belardo, Antonio Timperio, Anna Maria 2021-03-26 https://mts.intechopen.com/articles/show/title/from-targeted-quantification-to-untargeted-metabolomics https://doi.org/10.5772/intechopen.96852 en eng IntechOpen ISBN:978-1-83969-083-9 https://mts.intechopen.com/articles/show/title/from-targeted-quantification-to-untargeted-metabolomics doi:10.5772/intechopen.96852 https://creativecommons.org/licenses/by/3.0/ CC-BY https://www.intechopen.com/books/10220 Metabolomics - Methodology and Applications in Medical Sciences and Life Sciences Chapter, Part Of Book 2021 ftintech https://doi.org/10.5772/intechopen.96852 2021-11-13T20:05:35Z Metabolomics is an emerging and rapidly evolving technology tool, which involves quantitative and qualitative metabolite assessments science. It offers tremendous promise for different applications in various fields such as medical, environmental, nutrition, and agricultural sciences. Metabolomic approach is based on global identification of a high number of metabolites present in a biological fluid. This allows to characterize the metabolic profile of a given condition and to identify which metabolites or metabolite patterns may be useful in the discrimination between different groups. The use of one mass spectrometry (MS) platform from targeted quantification to untargeted metabolomics will make more efficient workflows in many fields and should allow projects to be more easily undertaken and realized. Metabolomics can be divided into non-targeted and targeted. The first one can analyze metabolites derived from the organisms comprehensively and systematically, so it is an unbiased metabolomics analysis that can discover new biomarkers. Targeted metabolomics, on the other hand, is the study and analysis of specific metabolites. Both have their own advantages and disadvantages, and are often used in combination for discovery and accurate weight determination of differential metabolites, and allow in-depth research and analysis of subsequent metabolic molecular markers. Targeted and non-targeted metabolomics are involved in food identification, disease research, animal model verification, biomarker discovery, disease diagnosis, drug development, drug screening, drug evaluation, clinical plant metabolism and microbial metabolism research. The aim of this chapter is to highlight the versatility of metabolomic analysis due to both the enormous variety of samples and the no strict barriers between quantitative and qualitative analysis. For this purpose, two examples from our group will be considered. Using non-targeted metabolomics in opposite Antarctic cryptoendolytic communities exposed to the sun, we revealed specific adaptations. Instead, through the targeted metabolomics applied to the urine during childbirth, we identified a different distribution of specific metabolites and the metabolic differences allowed us to discriminate between the two phases of labor, highlighting the metabolites most involved in the discrimination. The choice of these two approaches is to highlight that metabolomic analysis can be applied to any sample, even physiologically and metabolomically very distant, as can be microorganisms living on Antarctic rocks and biological fluids such as urine. Other/Unknown Material Antarc* Antarctic IntechOpen (E-Books) Antarctic
institution Open Polar
collection IntechOpen (E-Books)
op_collection_id ftintech
language English
topic Metabolomics - Methodology and Applications in Medical Sciences and Life Sciences
spellingShingle Metabolomics - Methodology and Applications in Medical Sciences and Life Sciences
Lelli, Veronica
Belardo, Antonio
Timperio, Anna Maria
From Targeted Quantification to Untargeted Metabolomics
topic_facet Metabolomics - Methodology and Applications in Medical Sciences and Life Sciences
description Metabolomics is an emerging and rapidly evolving technology tool, which involves quantitative and qualitative metabolite assessments science. It offers tremendous promise for different applications in various fields such as medical, environmental, nutrition, and agricultural sciences. Metabolomic approach is based on global identification of a high number of metabolites present in a biological fluid. This allows to characterize the metabolic profile of a given condition and to identify which metabolites or metabolite patterns may be useful in the discrimination between different groups. The use of one mass spectrometry (MS) platform from targeted quantification to untargeted metabolomics will make more efficient workflows in many fields and should allow projects to be more easily undertaken and realized. Metabolomics can be divided into non-targeted and targeted. The first one can analyze metabolites derived from the organisms comprehensively and systematically, so it is an unbiased metabolomics analysis that can discover new biomarkers. Targeted metabolomics, on the other hand, is the study and analysis of specific metabolites. Both have their own advantages and disadvantages, and are often used in combination for discovery and accurate weight determination of differential metabolites, and allow in-depth research and analysis of subsequent metabolic molecular markers. Targeted and non-targeted metabolomics are involved in food identification, disease research, animal model verification, biomarker discovery, disease diagnosis, drug development, drug screening, drug evaluation, clinical plant metabolism and microbial metabolism research. The aim of this chapter is to highlight the versatility of metabolomic analysis due to both the enormous variety of samples and the no strict barriers between quantitative and qualitative analysis. For this purpose, two examples from our group will be considered. Using non-targeted metabolomics in opposite Antarctic cryptoendolytic communities exposed to the sun, we revealed specific adaptations. Instead, through the targeted metabolomics applied to the urine during childbirth, we identified a different distribution of specific metabolites and the metabolic differences allowed us to discriminate between the two phases of labor, highlighting the metabolites most involved in the discrimination. The choice of these two approaches is to highlight that metabolomic analysis can be applied to any sample, even physiologically and metabolomically very distant, as can be microorganisms living on Antarctic rocks and biological fluids such as urine.
format Other/Unknown Material
author Lelli, Veronica
Belardo, Antonio
Timperio, Anna Maria
author_facet Lelli, Veronica
Belardo, Antonio
Timperio, Anna Maria
author_sort Lelli, Veronica
title From Targeted Quantification to Untargeted Metabolomics
title_short From Targeted Quantification to Untargeted Metabolomics
title_full From Targeted Quantification to Untargeted Metabolomics
title_fullStr From Targeted Quantification to Untargeted Metabolomics
title_full_unstemmed From Targeted Quantification to Untargeted Metabolomics
title_sort from targeted quantification to untargeted metabolomics
publisher IntechOpen
publishDate 2021
url https://mts.intechopen.com/articles/show/title/from-targeted-quantification-to-untargeted-metabolomics
https://doi.org/10.5772/intechopen.96852
geographic Antarctic
geographic_facet Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_source https://www.intechopen.com/books/10220
op_relation ISBN:978-1-83969-083-9
https://mts.intechopen.com/articles/show/title/from-targeted-quantification-to-untargeted-metabolomics
doi:10.5772/intechopen.96852
op_rights https://creativecommons.org/licenses/by/3.0/
op_rightsnorm CC-BY
op_doi https://doi.org/10.5772/intechopen.96852
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