Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans
Vibrio parahaemolyticus {Vp) is a marine bacterium considered as a leading cause of gasiroenteritis associated with raw or undercooked seafood consumption in humans worldwide.Isolatiod of Vp strains lacking known vimlence factors (TDH, TRH, ty pe I II secretion system (TsSS)) in clinical cases highl...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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HAL CCSD
2022
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Online Access: | https://theses.hal.science/tel-04213596 https://theses.hal.science/tel-04213596/document https://theses.hal.science/tel-04213596/file/These-2022-SML-Microbiologie-SOREE_Marion.pdf |
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ftinsu:oai:HAL:tel-04213596v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Vibrio parehaemolyticus Virulence Genomic Crassostrea gigas Accumulation Depuration Vibrio parahaemolyticus Génomique Dépuration [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
Vibrio parehaemolyticus Virulence Genomic Crassostrea gigas Accumulation Depuration Vibrio parahaemolyticus Génomique Dépuration [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Sorée, Marion Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
topic_facet |
Vibrio parehaemolyticus Virulence Genomic Crassostrea gigas Accumulation Depuration Vibrio parahaemolyticus Génomique Dépuration [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
Vibrio parahaemolyticus {Vp) is a marine bacterium considered as a leading cause of gasiroenteritis associated with raw or undercooked seafood consumption in humans worldwide.Isolatiod of Vp strains lacking known vimlence factors (TDH, TRH, ty pe I II secretion system (TsSS)) in clinical cases highlighted the need to better characterize these facrors and their expressies in in vivo models. Moreover, factorsassociated to the host, the oyster, Crassostrea gigas, should be considered to better accumulation and depuration of Vp from their tissues. The objectives of this thesis were to better characterize the pathogenic potential of environmental strains of Vp and to identify factors associated to oyster which could impact the contamination levels of these bacteria in their tissues.The genomic analysis combined to phenotypic characterization of virulence in the infant rabbit and Galleria mellonella larvae models revealed that, th e TDH does not seem to be the most relevant indicator of Vp pathogenicity, and that larvae mortality was probably explained by Vp’s T3SS -1. These are very interesting new observations which need do be further addressed. Experiments on C.gigas showed that oysters grown in secured structures accumulated experimentally more Vp than oysters grown in intertidal environment and that the ploidy level of oysters (dipioid vs. triploid) did not impact the natural contamination and the experimental accumulation and depuration of Vp.Finally, the use of Lactic acid bacteria which inhibited the growth of Vp in vitro, provided promising results concerningVp elimination during oyster depuration. Vibrio parahaemolyticus (Vp) est une bactérie marine considérée comme la principale cause bactérienne de gastroentérites liées à la consommation de produits de la mer crus ou insuffisamment cuits chez l’Homme dans le monde entier. L’absence de facteurs de virulence connue (TDH, TRH, système de sécrétion de type III (T3SS)) chez des souches Vp isolées de cas cliniques souligne la nécessité de mieux caractériser ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Université de Bretagne occidentale - Brest Dominique Hervio-Heath |
format |
Doctoral or Postdoctoral Thesis |
author |
Sorée, Marion |
author_facet |
Sorée, Marion |
author_sort |
Sorée, Marion |
title |
Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
title_short |
Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
title_full |
Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
title_fullStr |
Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
title_full_unstemmed |
Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
title_sort |
towards a better overview of virulence and risk management of vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://theses.hal.science/tel-04213596 https://theses.hal.science/tel-04213596/document https://theses.hal.science/tel-04213596/file/These-2022-SML-Microbiologie-SOREE_Marion.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
https://theses.hal.science/tel-04213596 Microbiology and Parasitology. Université de Bretagne occidentale - Brest, 2022. English. ⟨NNT : 2022BRES0073⟩ |
op_relation |
NNT: 2022BRES0073 tel-04213596 https://theses.hal.science/tel-04213596 https://theses.hal.science/tel-04213596/document https://theses.hal.science/tel-04213596/file/These-2022-SML-Microbiologie-SOREE_Marion.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1790599356625190912 |
spelling |
ftinsu:oai:HAL:tel-04213596v1 2024-02-11T10:03:11+01:00 Towards a better overview of virulence and risk management of Vibrio parahaemolyticus, a marine bacterium potentially pathogenic for humans Vers une meilleure approche de la virulence et de la gestion du risque de Vibrio parahaemolyticus, bactérie marine potentiellement pathogène pour l’Homme Sorée, Marion Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Université de Bretagne occidentale - Brest Dominique Hervio-Heath 2022-10-28 https://theses.hal.science/tel-04213596 https://theses.hal.science/tel-04213596/document https://theses.hal.science/tel-04213596/file/These-2022-SML-Microbiologie-SOREE_Marion.pdf en eng HAL CCSD NNT: 2022BRES0073 tel-04213596 https://theses.hal.science/tel-04213596 https://theses.hal.science/tel-04213596/document https://theses.hal.science/tel-04213596/file/These-2022-SML-Microbiologie-SOREE_Marion.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-04213596 Microbiology and Parasitology. Université de Bretagne occidentale - Brest, 2022. English. ⟨NNT : 2022BRES0073⟩ Vibrio parehaemolyticus Virulence Genomic Crassostrea gigas Accumulation Depuration Vibrio parahaemolyticus Génomique Dépuration [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/doctoralThesis Theses 2022 ftinsu 2024-01-24T17:26:06Z Vibrio parahaemolyticus {Vp) is a marine bacterium considered as a leading cause of gasiroenteritis associated with raw or undercooked seafood consumption in humans worldwide.Isolatiod of Vp strains lacking known vimlence factors (TDH, TRH, ty pe I II secretion system (TsSS)) in clinical cases highlighted the need to better characterize these facrors and their expressies in in vivo models. Moreover, factorsassociated to the host, the oyster, Crassostrea gigas, should be considered to better accumulation and depuration of Vp from their tissues. The objectives of this thesis were to better characterize the pathogenic potential of environmental strains of Vp and to identify factors associated to oyster which could impact the contamination levels of these bacteria in their tissues.The genomic analysis combined to phenotypic characterization of virulence in the infant rabbit and Galleria mellonella larvae models revealed that, th e TDH does not seem to be the most relevant indicator of Vp pathogenicity, and that larvae mortality was probably explained by Vp’s T3SS -1. These are very interesting new observations which need do be further addressed. Experiments on C.gigas showed that oysters grown in secured structures accumulated experimentally more Vp than oysters grown in intertidal environment and that the ploidy level of oysters (dipioid vs. triploid) did not impact the natural contamination and the experimental accumulation and depuration of Vp.Finally, the use of Lactic acid bacteria which inhibited the growth of Vp in vitro, provided promising results concerningVp elimination during oyster depuration. Vibrio parahaemolyticus (Vp) est une bactérie marine considérée comme la principale cause bactérienne de gastroentérites liées à la consommation de produits de la mer crus ou insuffisamment cuits chez l’Homme dans le monde entier. L’absence de facteurs de virulence connue (TDH, TRH, système de sécrétion de type III (T3SS)) chez des souches Vp isolées de cas cliniques souligne la nécessité de mieux caractériser ... Doctoral or Postdoctoral Thesis Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU |