Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1
In France, Ostreid herpesvirus type 1 (OsHV-1), can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas. Susceptibility differences to infection were observed in this species. Previous work suggested that the genetic basis and the survival animals to infection were...
Main Author: | |
---|---|
Other Authors: | , , , , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
Published: |
HAL CCSD
2014
|
Subjects: | |
Online Access: | https://theses.hal.science/tel-01192622 https://theses.hal.science/tel-01192622/document https://theses.hal.science/tel-01192622/file/2015theseSegarraA.pdf |
id |
ftinsu:oai:HAL:tel-01192622v1 |
---|---|
record_format |
openpolar |
spelling |
ftinsu:oai:HAL:tel-01192622v1 2024-02-11T10:03:03+01:00 Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 Étude des interactions hôte/virus chez l’huître creuse, Crassostrea gigas, et son virus Ostreid herpesvirus 1 Segarra, Amélie Laboratoire de Biotechnologie et Chimie Marines (LBCM) Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Université de Bretagne Sud Nathalie Bourgougnon Tristan Renault 2014-11-14 https://theses.hal.science/tel-01192622 https://theses.hal.science/tel-01192622/document https://theses.hal.science/tel-01192622/file/2015theseSegarraA.pdf fr fre HAL CCSD NNT: 2014LORIS344 tel-01192622 https://theses.hal.science/tel-01192622 https://theses.hal.science/tel-01192622/document https://theses.hal.science/tel-01192622/file/2015theseSegarraA.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-01192622 Biologie animale. Université de Bretagne Sud, 2014. Français. ⟨NNT : 2014LORIS344⟩ Interaction host-Virus Pacific oyster Real-Time PCR OsHV-1 Bivalve PCR en temps réel [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology info:eu-repo/semantics/doctoralThesis Theses 2014 ftinsu 2024-01-24T17:39:36Z In France, Ostreid herpesvirus type 1 (OsHV-1), can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas. Susceptibility differences to infection were observed in this species. Previous work suggested that the genetic basis and the survival animals to infection were related. In this context, the main objective of this thesis was to understand the interactions between oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Our results shows that the virus is able to replicate in the host regardless of its stage of development or its susceptible. However, multiplication kinetic is faster in susceptible individuals compared to less susceptible individuals. After a active replication, it would appear that the virus is no detectable in survival individuals. This observation suggests (i) a remission with elimination of the virus or (ii) a virus persistence without detectable symptoms. These results highlight the ability of the virus circulating in the host without causing mortality. These individuals can excrete viral particles and interfere with the infection process in field. All these results represent a first contribution to the understanding of OsHV-1 cycle in Pacific oysters, particularly at the molecular level. Le virus ostreid herpesvirus type 1 (OsHV-1), peut être considéré comme un des agents infectieux majeur affectant les élevages d’huîtres creuses, Crassostrea gigas, en France. Des différences de sensibilité à l’infection ont également été observées au sein de cette espèce. Des travaux précédents suggèrent un lien entre la base génétique et la survie des animaux face à l’infection. Dans ce contexte, l’objectif principal du travail de thèse était de mieux comprendre les interactions entre l’huître creuse et OsHV-1, et plus particulièrement, les bases moléculaires du cycle viral. Nos résultats montre que le virus est capable de se répliquer chez l’hôte quel que soit son stade de développement, et sa sensibilité. Cependant, la cinétique de multiplication ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
French |
topic |
Interaction host-Virus Pacific oyster Real-Time PCR OsHV-1 Bivalve PCR en temps réel [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
spellingShingle |
Interaction host-Virus Pacific oyster Real-Time PCR OsHV-1 Bivalve PCR en temps réel [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Segarra, Amélie Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 |
topic_facet |
Interaction host-Virus Pacific oyster Real-Time PCR OsHV-1 Bivalve PCR en temps réel [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
description |
In France, Ostreid herpesvirus type 1 (OsHV-1), can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas. Susceptibility differences to infection were observed in this species. Previous work suggested that the genetic basis and the survival animals to infection were related. In this context, the main objective of this thesis was to understand the interactions between oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Our results shows that the virus is able to replicate in the host regardless of its stage of development or its susceptible. However, multiplication kinetic is faster in susceptible individuals compared to less susceptible individuals. After a active replication, it would appear that the virus is no detectable in survival individuals. This observation suggests (i) a remission with elimination of the virus or (ii) a virus persistence without detectable symptoms. These results highlight the ability of the virus circulating in the host without causing mortality. These individuals can excrete viral particles and interfere with the infection process in field. All these results represent a first contribution to the understanding of OsHV-1 cycle in Pacific oysters, particularly at the molecular level. Le virus ostreid herpesvirus type 1 (OsHV-1), peut être considéré comme un des agents infectieux majeur affectant les élevages d’huîtres creuses, Crassostrea gigas, en France. Des différences de sensibilité à l’infection ont également été observées au sein de cette espèce. Des travaux précédents suggèrent un lien entre la base génétique et la survie des animaux face à l’infection. Dans ce contexte, l’objectif principal du travail de thèse était de mieux comprendre les interactions entre l’huître creuse et OsHV-1, et plus particulièrement, les bases moléculaires du cycle viral. Nos résultats montre que le virus est capable de se répliquer chez l’hôte quel que soit son stade de développement, et sa sensibilité. Cependant, la cinétique de multiplication ... |
author2 |
Laboratoire de Biotechnologie et Chimie Marines (LBCM) Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Université de Bretagne Sud Nathalie Bourgougnon Tristan Renault |
format |
Doctoral or Postdoctoral Thesis |
author |
Segarra, Amélie |
author_facet |
Segarra, Amélie |
author_sort |
Segarra, Amélie |
title |
Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 |
title_short |
Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 |
title_full |
Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 |
title_fullStr |
Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 |
title_full_unstemmed |
Studying interaction between the Pacific oyster, Crassostrea gigas, and Ostreid herpesvirus type 1 |
title_sort |
studying interaction between the pacific oyster, crassostrea gigas, and ostreid herpesvirus type 1 |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://theses.hal.science/tel-01192622 https://theses.hal.science/tel-01192622/document https://theses.hal.science/tel-01192622/file/2015theseSegarraA.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://theses.hal.science/tel-01192622 Biologie animale. Université de Bretagne Sud, 2014. Français. ⟨NNT : 2014LORIS344⟩ |
op_relation |
NNT: 2014LORIS344 tel-01192622 https://theses.hal.science/tel-01192622 https://theses.hal.science/tel-01192622/document https://theses.hal.science/tel-01192622/file/2015theseSegarraA.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1790599189453864960 |