Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas
The “cost of reproduction” is a concept defining that a high reproductive investment has a price that is paid later by an acceleration of senescence. That may translate tradeoff between reproduction and other physiological functions such as antioxidant defense. In the Pacific oyster Crassostrea giga...
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Other Authors: | , , , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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HAL CCSD
2012
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Subjects: | |
Online Access: | https://theses.hal.science/tel-00838503 https://theses.hal.science/tel-00838503/document https://theses.hal.science/tel-00838503/file/These-2012-EDSM-Biologie_marine-BEGUEL_Jean-Philippe.pdf |
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ftinsu:oai:HAL:tel-00838503v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
French |
topic |
Crassostrea gigas Reproductive investment ROS Oxidative stress Antioxidant capacity RNA interference Phenotypic manipulation Investissement reproducteur Stress oxydant Capacité antioxydante ARN interférence Manipulation phénotypique [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
Crassostrea gigas Reproductive investment ROS Oxidative stress Antioxidant capacity RNA interference Phenotypic manipulation Investissement reproducteur Stress oxydant Capacité antioxydante ARN interférence Manipulation phénotypique [SDV.BA]Life Sciences [q-bio]/Animal biology Béguel, Jean-Philippe Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas Reproductive investment ROS Oxidative stress Antioxidant capacity RNA interference Phenotypic manipulation Investissement reproducteur Stress oxydant Capacité antioxydante ARN interférence Manipulation phénotypique [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
The “cost of reproduction” is a concept defining that a high reproductive investment has a price that is paid later by an acceleration of senescence. That may translate tradeoff between reproduction and other physiological functions such as antioxidant defense. In the Pacific oyster Crassostrea gigas, reproduction is a major physiological function. In a study led to understand the summer mortalities affecting this species, a negative correlation between reproductive effort and survival was observed. Moreover, some antioxidant genes were identified as differentially expressed between lines of oysters selected for resistance or susceptibility to summer mortalities. Some studies suggest that the susceptibility to oxidative stress may represent a cost of reproduction taking part to the process of senescence. In this context, we analyzed the antioxidant capacity of oysters according to their reproductive investment. For this, the technique of RNA interference was used to manipulate the reproductive effort of oysters. The expression of the main antioxidant enzymes (transcript levels and enzyme activities) and the dosage of oxidative damages were then measured in different tissues and cells of the organism (gills, gonad, hemocytes and gametes). The results obtained in this thesis suggest that the antioxidant capacity of C. gigas is particularly effective and that reproduction alone is not sufficient to induce oxidative stress. This antioxidant capacity appears to be tissue-specific even cell-specific and glutathione metabolism would to play a major role in this protection. This resistance to oxidative stress would make C. gigas be a species particularly adapted to life in stressful environments. Le “coût de la reproduction” est un concept qui définit qu’un investissement à la reproduction élevé a un prix qui se paye ultérieurement par une accélération de la sénescence. Cela peut notamment traduire des compromis entre la reproduction et d’autres fonctions physiologiques comme la défense antioxydante. Chez l’huître ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Université de Bretagne occidentale - Brest Christophe Lambert |
format |
Doctoral or Postdoctoral Thesis |
author |
Béguel, Jean-Philippe |
author_facet |
Béguel, Jean-Philippe |
author_sort |
Béguel, Jean-Philippe |
title |
Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas |
title_short |
Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas |
title_full |
Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas |
title_fullStr |
Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas |
title_sort |
study of the antioxydant capacity in link with reproduction in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://theses.hal.science/tel-00838503 https://theses.hal.science/tel-00838503/document https://theses.hal.science/tel-00838503/file/These-2012-EDSM-Biologie_marine-BEGUEL_Jean-Philippe.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://theses.hal.science/tel-00838503 Biologie animale. Université de Bretagne occidentale - Brest, 2012. Français. ⟨NNT : 2012BRES0042⟩ |
op_relation |
NNT: 2012BRES0042 tel-00838503 https://theses.hal.science/tel-00838503 https://theses.hal.science/tel-00838503/document https://theses.hal.science/tel-00838503/file/These-2012-EDSM-Biologie_marine-BEGUEL_Jean-Philippe.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1790599189652045824 |
spelling |
ftinsu:oai:HAL:tel-00838503v1 2024-02-11T10:03:03+01:00 Study of the antioxydant capacity in link with reproduction in the Pacific oyster Crassostrea gigas Étude de la capacité antioxydante en lien avec la reproduction chez l'huître creuse Crassostrea gigas Béguel, Jean-Philippe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Université de Bretagne occidentale - Brest Christophe Lambert 2012-12-20 https://theses.hal.science/tel-00838503 https://theses.hal.science/tel-00838503/document https://theses.hal.science/tel-00838503/file/These-2012-EDSM-Biologie_marine-BEGUEL_Jean-Philippe.pdf fr fre HAL CCSD NNT: 2012BRES0042 tel-00838503 https://theses.hal.science/tel-00838503 https://theses.hal.science/tel-00838503/document https://theses.hal.science/tel-00838503/file/These-2012-EDSM-Biologie_marine-BEGUEL_Jean-Philippe.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-00838503 Biologie animale. Université de Bretagne occidentale - Brest, 2012. Français. ⟨NNT : 2012BRES0042⟩ Crassostrea gigas Reproductive investment ROS Oxidative stress Antioxidant capacity RNA interference Phenotypic manipulation Investissement reproducteur Stress oxydant Capacité antioxydante ARN interférence Manipulation phénotypique [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/doctoralThesis Theses 2012 ftinsu 2024-01-24T17:22:53Z The “cost of reproduction” is a concept defining that a high reproductive investment has a price that is paid later by an acceleration of senescence. That may translate tradeoff between reproduction and other physiological functions such as antioxidant defense. In the Pacific oyster Crassostrea gigas, reproduction is a major physiological function. In a study led to understand the summer mortalities affecting this species, a negative correlation between reproductive effort and survival was observed. Moreover, some antioxidant genes were identified as differentially expressed between lines of oysters selected for resistance or susceptibility to summer mortalities. Some studies suggest that the susceptibility to oxidative stress may represent a cost of reproduction taking part to the process of senescence. In this context, we analyzed the antioxidant capacity of oysters according to their reproductive investment. For this, the technique of RNA interference was used to manipulate the reproductive effort of oysters. The expression of the main antioxidant enzymes (transcript levels and enzyme activities) and the dosage of oxidative damages were then measured in different tissues and cells of the organism (gills, gonad, hemocytes and gametes). The results obtained in this thesis suggest that the antioxidant capacity of C. gigas is particularly effective and that reproduction alone is not sufficient to induce oxidative stress. This antioxidant capacity appears to be tissue-specific even cell-specific and glutathione metabolism would to play a major role in this protection. This resistance to oxidative stress would make C. gigas be a species particularly adapted to life in stressful environments. Le “coût de la reproduction” est un concept qui définit qu’un investissement à la reproduction élevé a un prix qui se paye ultérieurement par une accélération de la sénescence. Cela peut notamment traduire des compromis entre la reproduction et d’autres fonctions physiologiques comme la défense antioxydante. Chez l’huître ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific |