Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples
International audience Four different communities and one culture of autotrophic microbial assemblages were obtained by incubation of samples collected from high elevation snow in the Alps (Mt. Blanc area) and the Andes (Nevado Illimani summit, Bolivia), from Antarctic aerosol (French station Dumont...
Published in: | Biogeosciences |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2009
|
Subjects: | |
Online Access: | https://insu.hal.science/insu-00420852 https://insu.hal.science/insu-00420852/document https://insu.hal.science/insu-00420852/file/bg-6-33-2009.pdf https://doi.org/10.5194/bg-6-33-2009 |
id |
ftinsu:oai:HAL:insu-00420852v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
[SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology [SDE.MCG]Environmental Sciences/Global Changes |
spellingShingle |
[SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology [SDE.MCG]Environmental Sciences/Global Changes Gonzalez-Toril, E. Amils, R. Delmas, Robert Petit, Jean-Robert Komarek, J. Elster, J. Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples |
topic_facet |
[SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology [SDE.MCG]Environmental Sciences/Global Changes |
description |
International audience Four different communities and one culture of autotrophic microbial assemblages were obtained by incubation of samples collected from high elevation snow in the Alps (Mt. Blanc area) and the Andes (Nevado Illimani summit, Bolivia), from Antarctic aerosol (French station Dumont d'Urville) and a maritime Antarctic soil (King George Island, South Shetlands, Uruguay Station Artigas), in a minimal mineral (oligotrophic) media. Molecular analysis of more than 200 16S rRNA gene sequences showed that all cultured cells belong to the Bacteria domain. Phylogenetic comparison with the currently available rDNA database allowed sequences belonging to Proteobacteria (Alpha-, Betaand Gamma-proteobacteria) , Actinobacteria and Bacteroidetes phyla to be identified. The Andes snow culture was the richest in bacterial diversity (eight microorganisms identified) and the marine Antarctic soil the poorest (only one). Snow samples from Col du Midi (Alps) and the Andes shared the highest number of identified microorganisms (Agrobacterium, Limnobacter, Aquiflexus and two uncultured Alphaproteobacteria clones). These two sampling sites also shared four sequences with the Antarctic aerosol sample (Limnobacter, Pseudonocardia and an uncultured Alphaproteobacteria clone). The only microorganism identified in the Antarctica soil (Brevundimonas sp.) was also detected in the Antarctic aerosol. Most of the identified microorganisms had been detected previously in cold environments, marine sediments soils and rocks. Air current dispersal is the best model to explain the presence of very specific microorganisms, like those identified in this work, in environments very distant and very different from each other. |
author2 |
Centro de Astrobiologia Madrid (CAB) Instituto Nacional de Técnica Aeroespacial (INTA)-Consejo Superior de Investigaciones Cientificas = Spanish National Research Council (CSIC) Centro de Biología Molecular Severo Ochoa Madrid (CBMSO) Consejo Superior de Investigaciones Cientificas = Spanish National Research Council (CSIC)-Universidad Autónoma de Madrid (UAM) Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institute of Botany Czech Academy of Sciences Prague (CAS) grants from the Spanish Ministerio de Educaci'on y Ciencia (grant CGL2006/02534/BOS), the Ministry of Education of the Czech Republic (Kontakt - ME 934, ME 945 and project GA CR 206/05/0253) and the French-Czech cooperative research programme Barrande No. 99054. |
format |
Article in Journal/Newspaper |
author |
Gonzalez-Toril, E. Amils, R. Delmas, Robert Petit, Jean-Robert Komarek, J. Elster, J. |
author_facet |
Gonzalez-Toril, E. Amils, R. Delmas, Robert Petit, Jean-Robert Komarek, J. Elster, J. |
author_sort |
Gonzalez-Toril, E. |
title |
Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples |
title_short |
Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples |
title_full |
Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples |
title_fullStr |
Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples |
title_full_unstemmed |
Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples |
title_sort |
bacterial diversity of autotrophic enriched cultures from remote, glacial antarctic, alpine and andean aerosol, snow and soil samples |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://insu.hal.science/insu-00420852 https://insu.hal.science/insu-00420852/document https://insu.hal.science/insu-00420852/file/bg-6-33-2009.pdf https://doi.org/10.5194/bg-6-33-2009 |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
ISSN: 1726-4170 EISSN: 1726-4189 Biogeosciences https://insu.hal.science/insu-00420852 Biogeosciences, 2009, 6 (1), pp.33-44. ⟨10.5194/bg-6-33-2009⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-6-33-2009 insu-00420852 https://insu.hal.science/insu-00420852 https://insu.hal.science/insu-00420852/document https://insu.hal.science/insu-00420852/file/bg-6-33-2009.pdf doi:10.5194/bg-6-33-2009 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.5194/bg-6-33-2009 |
container_title |
Biogeosciences |
container_volume |
6 |
container_issue |
1 |
container_start_page |
33 |
op_container_end_page |
44 |
_version_ |
1797573284329422848 |
spelling |
ftinsu:oai:HAL:insu-00420852v1 2024-04-28T08:01:37+00:00 Bacterial diversity of autotrophic enriched cultures from remote, glacial Antarctic, Alpine and Andean aerosol, snow and soil samples Gonzalez-Toril, E. Amils, R. Delmas, Robert Petit, Jean-Robert Komarek, J. Elster, J. Centro de Astrobiologia Madrid (CAB) Instituto Nacional de Técnica Aeroespacial (INTA)-Consejo Superior de Investigaciones Cientificas = Spanish National Research Council (CSIC) Centro de Biología Molecular Severo Ochoa Madrid (CBMSO) Consejo Superior de Investigaciones Cientificas = Spanish National Research Council (CSIC)-Universidad Autónoma de Madrid (UAM) Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institute of Botany Czech Academy of Sciences Prague (CAS) grants from the Spanish Ministerio de Educaci'on y Ciencia (grant CGL2006/02534/BOS), the Ministry of Education of the Czech Republic (Kontakt - ME 934, ME 945 and project GA CR 206/05/0253) and the French-Czech cooperative research programme Barrande No. 99054. 2009-01-08 https://insu.hal.science/insu-00420852 https://insu.hal.science/insu-00420852/document https://insu.hal.science/insu-00420852/file/bg-6-33-2009.pdf https://doi.org/10.5194/bg-6-33-2009 en eng HAL CCSD European Geosciences Union info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-6-33-2009 insu-00420852 https://insu.hal.science/insu-00420852 https://insu.hal.science/insu-00420852/document https://insu.hal.science/insu-00420852/file/bg-6-33-2009.pdf doi:10.5194/bg-6-33-2009 info:eu-repo/semantics/OpenAccess ISSN: 1726-4170 EISSN: 1726-4189 Biogeosciences https://insu.hal.science/insu-00420852 Biogeosciences, 2009, 6 (1), pp.33-44. ⟨10.5194/bg-6-33-2009⟩ [SDU.STU.GL]Sciences of the Universe [physics]/Earth Sciences/Glaciology [SDE.MCG]Environmental Sciences/Global Changes info:eu-repo/semantics/article Journal articles 2009 ftinsu https://doi.org/10.5194/bg-6-33-2009 2024-04-05T00:28:17Z International audience Four different communities and one culture of autotrophic microbial assemblages were obtained by incubation of samples collected from high elevation snow in the Alps (Mt. Blanc area) and the Andes (Nevado Illimani summit, Bolivia), from Antarctic aerosol (French station Dumont d'Urville) and a maritime Antarctic soil (King George Island, South Shetlands, Uruguay Station Artigas), in a minimal mineral (oligotrophic) media. Molecular analysis of more than 200 16S rRNA gene sequences showed that all cultured cells belong to the Bacteria domain. Phylogenetic comparison with the currently available rDNA database allowed sequences belonging to Proteobacteria (Alpha-, Betaand Gamma-proteobacteria) , Actinobacteria and Bacteroidetes phyla to be identified. The Andes snow culture was the richest in bacterial diversity (eight microorganisms identified) and the marine Antarctic soil the poorest (only one). Snow samples from Col du Midi (Alps) and the Andes shared the highest number of identified microorganisms (Agrobacterium, Limnobacter, Aquiflexus and two uncultured Alphaproteobacteria clones). These two sampling sites also shared four sequences with the Antarctic aerosol sample (Limnobacter, Pseudonocardia and an uncultured Alphaproteobacteria clone). The only microorganism identified in the Antarctica soil (Brevundimonas sp.) was also detected in the Antarctic aerosol. Most of the identified microorganisms had been detected previously in cold environments, marine sediments soils and rocks. Air current dispersal is the best model to explain the presence of very specific microorganisms, like those identified in this work, in environments very distant and very different from each other. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Institut national des sciences de l'Univers: HAL-INSU Biogeosciences 6 1 33 44 |