Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas )

• Published in: Environmental Microbiology
• Main Authors: Sorée, Marion, Delavat, François, Lambert, Christophe, Lozach, Solen, Papin, Mathias, Petton, Bruno, Passerini, Delphine, Dégremont, Lionel, Hervio Heath, Dominique
• Other Authors: Unité Microbiologie Aliment Santé Environnement (MASAE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Unité en Sciences Biologiques et Biotechnologies de Nantes (US2B), Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Unité Expérimentale Mollusques Marins Atlantique (EMMA), Unité Adaptation et Santé des Invertébrés Marins (ASIM)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2022
• Subjects: [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology; [SDV.EE.IEO]Life Sciences [q-bio]/Ecology; environment/Symbiosis; Crassostrea gigas
• Online Access: https://hal.science/hal-04202208; https://hal.science/hal-04202208/document; https://hal.science/hal-04202208/file/Life%20history%20of%20oysters%20influences%20Vibrio%20parahaemolyticus%20accumulation%20in%20Pacific%20oysters%20%28Crassostrea%20gigas%29.%20Hervio%20Heath%202022.pdf; https://doi.org/10.1111/1462-2920.15996

International audience Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V . parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V . parahaemolyticus accumulation and depuration in adult Crassostrea gigas , we developed a GFP‐tagged V . parahaemolyticus strain (IFVp201‐ gfp + ), as well as a rapid and efficient quantification method in C . gigas oysters haemolymph by flow cytometry. Impact of the life history of C . gigas on accumulation and depuration of V . parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V . parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates.