Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling

International audience Iron (Fe) governs the cycling of organic carbon in large parts of the Southern Ocean. The strategies of diverse microbes to acquire the different chemical forms of Fe under seasonally changing organic carbon regimes remain, however, poorly understood. Here, we report high-reso...

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Published in:Environmental Microbiology
Main Authors: Zhang, Rui, Debeljak, Pavla, Blain, Stephane, Obernosterer, Ingrid
Other Authors: Laboratoire d'Océanographie Microbienne (LOMIC), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2023
Subjects:
Online Access:https://hal.science/hal-04093948
https://hal.science/hal-04093948/document
https://hal.science/hal-04093948/file/Seasonal%20shifts%20in%20Fe-acquisition%20strategies%20in%20Southern%20Ocean%20microbial%20communities%20revealed%20by%20metagenomics%20and%20autonomous%20sampling.pdf
https://doi.org/10.1111/1462-2920.16397
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spelling ftinsu:oai:HAL:hal-04093948v1 2024-02-11T10:08:45+01:00 Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling Zhang, Rui Debeljak, Pavla Blain, Stephane Obernosterer, Ingrid Laboratoire d'Océanographie Microbienne (LOMIC) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut de Systématique, Evolution, Biodiversité (ISYEB ) Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2023 https://hal.science/hal-04093948 https://hal.science/hal-04093948/document https://hal.science/hal-04093948/file/Seasonal%20shifts%20in%20Fe-acquisition%20strategies%20in%20Southern%20Ocean%20microbial%20communities%20revealed%20by%20metagenomics%20and%20autonomous%20sampling.pdf https://doi.org/10.1111/1462-2920.16397 en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.16397 hal-04093948 https://hal.science/hal-04093948 https://hal.science/hal-04093948/document https://hal.science/hal-04093948/file/Seasonal%20shifts%20in%20Fe-acquisition%20strategies%20in%20Southern%20Ocean%20microbial%20communities%20revealed%20by%20metagenomics%20and%20autonomous%20sampling.pdf doi:10.1111/1462-2920.16397 info:eu-repo/semantics/OpenAccess ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.science/hal-04093948 Environmental Microbiology, In press, ⟨10.1111/1462-2920.16397⟩ marine prokaryotes iron and organic carbon acquisition metagenomics MAGs Southern Ocean autonomous sampler [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2023 ftinsu https://doi.org/10.1111/1462-2920.16397 2024-01-24T17:26:46Z International audience Iron (Fe) governs the cycling of organic carbon in large parts of the Southern Ocean. The strategies of diverse microbes to acquire the different chemical forms of Fe under seasonally changing organic carbon regimes remain, however, poorly understood. Here, we report high-resolution seasonal metagenomic observations from the region off Kerguelen Island (Indian Sector of the Southern Ocean) where natural Fe-fertilization induces consecutive spring and summer phytoplankton blooms. Our data illustrate pronounced, but distinct seasonal patterns in the abundance of genes implicated in the transport of different forms of Fe and organic substrates, of siderophore biosynthesis and carbohydrate-active enzymes. The seasonal dynamics suggest a temporal decoupling in the prokaryotic requirements of Fe and organic carbon during the spring phytoplankton bloom and a concerted access to these resources after the summer bloom. Taxonomic assignments revealed differences in the prokaryotic groups harbouring genes of a given Fe-related category and pronounced seasonal successions were observed. Using MAGs we could decipher the respective Fe- and organic substrate-related genes of individual taxa assigned to abundant groups. The ecological strategies related to Fe-acquisition provide insights on how this element could shape microbial community composition with potential implications on organic matter transformations in the Southern Ocean Article in Journal/Newspaper Southern Ocean Institut national des sciences de l'Univers: HAL-INSU Indian Kerguelen Kerguelen Island ENVELOPE(69.500,69.500,-49.250,-49.250) Southern Ocean Environmental Microbiology
institution Open Polar
collection Institut national des sciences de l'Univers: HAL-INSU
op_collection_id ftinsu
language English
topic marine prokaryotes
iron and organic carbon acquisition
metagenomics
MAGs
Southern Ocean
autonomous sampler
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
spellingShingle marine prokaryotes
iron and organic carbon acquisition
metagenomics
MAGs
Southern Ocean
autonomous sampler
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Zhang, Rui
Debeljak, Pavla
Blain, Stephane
Obernosterer, Ingrid
Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling
topic_facet marine prokaryotes
iron and organic carbon acquisition
metagenomics
MAGs
Southern Ocean
autonomous sampler
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
description International audience Iron (Fe) governs the cycling of organic carbon in large parts of the Southern Ocean. The strategies of diverse microbes to acquire the different chemical forms of Fe under seasonally changing organic carbon regimes remain, however, poorly understood. Here, we report high-resolution seasonal metagenomic observations from the region off Kerguelen Island (Indian Sector of the Southern Ocean) where natural Fe-fertilization induces consecutive spring and summer phytoplankton blooms. Our data illustrate pronounced, but distinct seasonal patterns in the abundance of genes implicated in the transport of different forms of Fe and organic substrates, of siderophore biosynthesis and carbohydrate-active enzymes. The seasonal dynamics suggest a temporal decoupling in the prokaryotic requirements of Fe and organic carbon during the spring phytoplankton bloom and a concerted access to these resources after the summer bloom. Taxonomic assignments revealed differences in the prokaryotic groups harbouring genes of a given Fe-related category and pronounced seasonal successions were observed. Using MAGs we could decipher the respective Fe- and organic substrate-related genes of individual taxa assigned to abundant groups. The ecological strategies related to Fe-acquisition provide insights on how this element could shape microbial community composition with potential implications on organic matter transformations in the Southern Ocean
author2 Laboratoire d'Océanographie Microbienne (LOMIC)
Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB)
Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Institut de Systématique, Evolution, Biodiversité (ISYEB )
Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE)
Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)
format Article in Journal/Newspaper
author Zhang, Rui
Debeljak, Pavla
Blain, Stephane
Obernosterer, Ingrid
author_facet Zhang, Rui
Debeljak, Pavla
Blain, Stephane
Obernosterer, Ingrid
author_sort Zhang, Rui
title Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling
title_short Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling
title_full Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling
title_fullStr Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling
title_full_unstemmed Seasonal shifts in Fe-acquisition strategies in Southern Ocean microbial communities revealed by metagenomics and autonomous sampling
title_sort seasonal shifts in fe-acquisition strategies in southern ocean microbial communities revealed by metagenomics and autonomous sampling
publisher HAL CCSD
publishDate 2023
url https://hal.science/hal-04093948
https://hal.science/hal-04093948/document
https://hal.science/hal-04093948/file/Seasonal%20shifts%20in%20Fe-acquisition%20strategies%20in%20Southern%20Ocean%20microbial%20communities%20revealed%20by%20metagenomics%20and%20autonomous%20sampling.pdf
https://doi.org/10.1111/1462-2920.16397
long_lat ENVELOPE(69.500,69.500,-49.250,-49.250)
geographic Indian
Kerguelen
Kerguelen Island
Southern Ocean
geographic_facet Indian
Kerguelen
Kerguelen Island
Southern Ocean
genre Southern Ocean
genre_facet Southern Ocean
op_source ISSN: 1462-2912
EISSN: 1462-2920
Environmental Microbiology
https://hal.science/hal-04093948
Environmental Microbiology, In press, ⟨10.1111/1462-2920.16397⟩
op_relation info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.16397
hal-04093948
https://hal.science/hal-04093948
https://hal.science/hal-04093948/document
https://hal.science/hal-04093948/file/Seasonal%20shifts%20in%20Fe-acquisition%20strategies%20in%20Southern%20Ocean%20microbial%20communities%20revealed%20by%20metagenomics%20and%20autonomous%20sampling.pdf
doi:10.1111/1462-2920.16397
op_rights info:eu-repo/semantics/OpenAccess
op_doi https://doi.org/10.1111/1462-2920.16397
container_title Environmental Microbiology
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