Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas)

• Published in: Journal of Applied Microbiology
• Main Authors: Sorée, Marion, Kolypczuk, Laetitia, Hadjiev, Emilie, Lozach, Solen, Verrez-Bagnis, Véronique, Delbarre-Ladrat, Christine, Heath, Dominique, Hervio, Passerini, Delphine
• Other Authors: Unité Microbiologie Aliment Santé Environnement (MASAE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Région BretagneIfremer
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2022
• Subjects: V. parahaemolyticus lactic acid bacteria flow cytometry oyster depuration challenge test seafood safety; V. parahaemolyticus; lactic acid bacteria; flow cytometry; oyster depuration; challenge test; seafood safety; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; [SDV.EE.IEO]Life Sciences [q-bio]/Ecology; environment/Symbiosis; Crassostrea gigas
• Online Access: https://hal.univ-brest.fr/hal-04002464; https://doi.org/10.1093/jambio/lxac081

International audience Aims This study aims to assess the use of marine lactic acid bacteria (LAB) to reduce Vibrio parahaemolyticus levels during oyster depuration process. Methods and results The inhibitory effect of 30 marine LAB strains against V. parahaemolyticus strains was evaluated by in vitro assays. A total of three positive strains (Latilactobacillus sakei SF1583, Lactococcus lactis SF1945, and Vagococcus fluvialis CD264) were selected for V. parahaemolyticus levels reduction during oyster depuration. Pacific oysters Crassostrea gigas were artificially and independently contaminated by four GFP-labelled V. parahaemolyticus strains (IFVp201, IFVp69, IFVp195, and LMG2850 T) at 10 5 CFU ml −1 and then exposed by balneation to 10 6 CFU ml −1 of each LAB strains during 24 h, at 19 • C. Quantification of V. parahaemolyticus in haemolymph by flow cytometry revealed variations in natural depuration of the different V. parahaemolyticus strains alone. Furthermore, the addition of LABs improved up to 1-log bacteria ml −1 the reduction of IFVp201 concentration in comparison to the control condition. Conclusions Although further optimizations of procedure are needed, addition of marine LABs during oyster depuration may be an interesting strategy to reduce V. parahaemolyticus levels in Crassostrea gigas. Significance and impact of the study Our study provides promising ways to develop a depuration process, which could potentially be implemented in oyster farms.