Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes.
International audience BACKGROUND: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differenti...
Published in: | PLoS ONE |
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Main Authors: | , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2012
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Online Access: | https://hal.univ-brest.fr/hal-03779132 https://doi.org/10.1371/journal.pone.0036353 |
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Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
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language |
English |
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[SDE]Environmental Sciences |
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[SDE]Environmental Sciences Dheilly, Nolwenn, M. Lelong, Christophe Huvet, Arnaud Kellner, Kristell Dubos, Marie-Pierre Riviere, Guillaume Boudry, Pierre Favrel, Pascal Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience BACKGROUND: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. METHODOLOGY/PRINCIPAL FINDINGS: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. CONCLUSIONS/SIGNIFICANCE: This study allowed ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Dheilly, Nolwenn, M. Lelong, Christophe Huvet, Arnaud Kellner, Kristell Dubos, Marie-Pierre Riviere, Guillaume Boudry, Pierre Favrel, Pascal |
author_facet |
Dheilly, Nolwenn, M. Lelong, Christophe Huvet, Arnaud Kellner, Kristell Dubos, Marie-Pierre Riviere, Guillaume Boudry, Pierre Favrel, Pascal |
author_sort |
Dheilly, Nolwenn, M. |
title |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_short |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_full |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_fullStr |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_full_unstemmed |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_sort |
gametogenesis in the pacific oyster crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-03779132 https://doi.org/10.1371/journal.pone.0036353 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.univ-brest.fr/hal-03779132 PLoS ONE, 2012, 7 (5), pp.e36353. ⟨10.1371/journal.pone.0036353⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0036353 info:eu-repo/semantics/altIdentifier/pmid/22590533 hal-03779132 https://hal.univ-brest.fr/hal-03779132 doi:10.1371/journal.pone.0036353 PUBMED: 22590533 PUBMEDCENTRAL: PMC3348941 |
op_doi |
https://doi.org/10.1371/journal.pone.0036353 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
5 |
container_start_page |
e36353 |
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1790599305273278464 |
spelling |
ftinsu:oai:HAL:hal-03779132v1 2024-02-11T10:03:08+01:00 Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. Dheilly, Nolwenn, M. Lelong, Christophe Huvet, Arnaud Kellner, Kristell Dubos, Marie-Pierre Riviere, Guillaume Boudry, Pierre Favrel, Pascal Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) 2012 https://hal.univ-brest.fr/hal-03779132 https://doi.org/10.1371/journal.pone.0036353 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0036353 info:eu-repo/semantics/altIdentifier/pmid/22590533 hal-03779132 https://hal.univ-brest.fr/hal-03779132 doi:10.1371/journal.pone.0036353 PUBMED: 22590533 PUBMEDCENTRAL: PMC3348941 ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.univ-brest.fr/hal-03779132 PLoS ONE, 2012, 7 (5), pp.e36353. ⟨10.1371/journal.pone.0036353⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftinsu https://doi.org/10.1371/journal.pone.0036353 2024-01-24T17:28:33Z International audience BACKGROUND: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. METHODOLOGY/PRINCIPAL FINDINGS: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. CONCLUSIONS/SIGNIFICANCE: This study allowed ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific PLoS ONE 7 5 e36353 |