Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax)
International audience Since sensory system allows organisms to perceive and interact with their external environment, any disruption in their functioning may have detrimental consequences on their survival. Ocean acidification has been shown to potentially impair olfactory system in fish and it is...
Published in: | Marine Environmental Research |
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Main Authors: | , , , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2021
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Online Access: | https://hal.univ-brest.fr/hal-03343631 https://hal.univ-brest.fr/hal-03343631/document https://hal.univ-brest.fr/hal-03343631/file/86904.pdf https://doi.org/10.1016/j.marenvres.2021.105438 |
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Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
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language |
English |
topic |
Acidification Dicentrarchus labrax Expression pattern Intergenerational OMP [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDE]Environmental Sciences |
spellingShingle |
Acidification Dicentrarchus labrax Expression pattern Intergenerational OMP [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDE]Environmental Sciences Mazurais, David Neven, Carolin Servili, Arianna Vitré, Thomas Madec, Lauriane Collet, Sophie Zambonino-Infante, Jose-Luis Mark, Felix Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) |
topic_facet |
Acidification Dicentrarchus labrax Expression pattern Intergenerational OMP [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDE]Environmental Sciences |
description |
International audience Since sensory system allows organisms to perceive and interact with their external environment, any disruption in their functioning may have detrimental consequences on their survival. Ocean acidification has been shown to potentially impair olfactory system in fish and it is therefore essential to develop biological tools contributing to better characterize such effects. The olfactory marker protein (omp) gene is involved in the maturation and the activity of olfactory sensory neurons in vertebrates. In teleosts, two omp genes (ompa and ompb) originating from whole genome duplication have been identified. In this study, bioinformatic analysis allowed characterization of the ompa and ompb genes from the European seabass (Dicentrarchus labrax) genome. The European seabass ompa and ompb genes differ in deduced amino acid sequences and in their expression pattern throughout the tissues. While both ompa and ompb mRNA are strongly expressed in the olfactory epithelium, ompb expression was further observable in different brain areas while ompa expression was also detected in the eyes and in other peripheral tissues. Expression levels of ompa and ompb mRNA were investigated in adult seabass (4 years-old, F0) and in their offspring (F1) exposed to pH of 8 (control) or 7.6 (ocean acidification, OA). Under OA ompb mRNA was down-regulated while ompa mRNA was up-regulated in the olfactory epithelium of F0 adults, suggesting a long-term intragenerational OA-induced regulation of the olfactory sensory system. A shift in the expression profiles of both ompa and ompb mRNA was observed at early larval stages in F1 under OA, suggesting a disruption in the developmental process. Contrary to the F0, the expression of ompa and ompb mRNA was not anymore significantly regulated under OA in the olfactory epithelium of juvenile F1 fish. This work provides evidence for long-term impact of OA on sensorial system of European seabass as well as potential intergenerational acclimation of omp genes expression to OA in ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) |
format |
Article in Journal/Newspaper |
author |
Mazurais, David Neven, Carolin Servili, Arianna Vitré, Thomas Madec, Lauriane Collet, Sophie Zambonino-Infante, Jose-Luis Mark, Felix |
author_facet |
Mazurais, David Neven, Carolin Servili, Arianna Vitré, Thomas Madec, Lauriane Collet, Sophie Zambonino-Infante, Jose-Luis Mark, Felix |
author_sort |
Mazurais, David |
title |
Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) |
title_short |
Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) |
title_full |
Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) |
title_fullStr |
Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) |
title_full_unstemmed |
Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) |
title_sort |
effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in european seabass (dicentrarchus labrax) |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.univ-brest.fr/hal-03343631 https://hal.univ-brest.fr/hal-03343631/document https://hal.univ-brest.fr/hal-03343631/file/86904.pdf https://doi.org/10.1016/j.marenvres.2021.105438 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research https://hal.univ-brest.fr/hal-03343631 Marine Environmental Research, 2021, 170, pp.105438. ⟨10.1016/j.marenvres.2021.105438⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2021.105438 hal-03343631 https://hal.univ-brest.fr/hal-03343631 https://hal.univ-brest.fr/hal-03343631/document https://hal.univ-brest.fr/hal-03343631/file/86904.pdf doi:10.1016/j.marenvres.2021.105438 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.marenvres.2021.105438 |
container_title |
Marine Environmental Research |
container_volume |
170 |
container_start_page |
105438 |
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1790606020125392896 |
spelling |
ftinsu:oai:HAL:hal-03343631v1 2024-02-11T10:07:27+01:00 Effect of long-term intergenerational exposure to ocean acidification on ompa and ompb transcripts expression in European seabass (Dicentrarchus labrax) Mazurais, David Neven, Carolin Servili, Arianna Vitré, Thomas Madec, Lauriane Collet, Sophie Zambonino-Infante, Jose-Luis Mark, Felix Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) 2021-08 https://hal.univ-brest.fr/hal-03343631 https://hal.univ-brest.fr/hal-03343631/document https://hal.univ-brest.fr/hal-03343631/file/86904.pdf https://doi.org/10.1016/j.marenvres.2021.105438 en eng HAL CCSD Elsevier science info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2021.105438 hal-03343631 https://hal.univ-brest.fr/hal-03343631 https://hal.univ-brest.fr/hal-03343631/document https://hal.univ-brest.fr/hal-03343631/file/86904.pdf doi:10.1016/j.marenvres.2021.105438 info:eu-repo/semantics/OpenAccess ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research https://hal.univ-brest.fr/hal-03343631 Marine Environmental Research, 2021, 170, pp.105438. ⟨10.1016/j.marenvres.2021.105438⟩ Acidification Dicentrarchus labrax Expression pattern Intergenerational OMP [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2021 ftinsu https://doi.org/10.1016/j.marenvres.2021.105438 2024-01-24T17:26:52Z International audience Since sensory system allows organisms to perceive and interact with their external environment, any disruption in their functioning may have detrimental consequences on their survival. Ocean acidification has been shown to potentially impair olfactory system in fish and it is therefore essential to develop biological tools contributing to better characterize such effects. The olfactory marker protein (omp) gene is involved in the maturation and the activity of olfactory sensory neurons in vertebrates. In teleosts, two omp genes (ompa and ompb) originating from whole genome duplication have been identified. In this study, bioinformatic analysis allowed characterization of the ompa and ompb genes from the European seabass (Dicentrarchus labrax) genome. The European seabass ompa and ompb genes differ in deduced amino acid sequences and in their expression pattern throughout the tissues. While both ompa and ompb mRNA are strongly expressed in the olfactory epithelium, ompb expression was further observable in different brain areas while ompa expression was also detected in the eyes and in other peripheral tissues. Expression levels of ompa and ompb mRNA were investigated in adult seabass (4 years-old, F0) and in their offspring (F1) exposed to pH of 8 (control) or 7.6 (ocean acidification, OA). Under OA ompb mRNA was down-regulated while ompa mRNA was up-regulated in the olfactory epithelium of F0 adults, suggesting a long-term intragenerational OA-induced regulation of the olfactory sensory system. A shift in the expression profiles of both ompa and ompb mRNA was observed at early larval stages in F1 under OA, suggesting a disruption in the developmental process. Contrary to the F0, the expression of ompa and ompb mRNA was not anymore significantly regulated under OA in the olfactory epithelium of juvenile F1 fish. This work provides evidence for long-term impact of OA on sensorial system of European seabass as well as potential intergenerational acclimation of omp genes expression to OA in ... Article in Journal/Newspaper Ocean acidification Institut national des sciences de l'Univers: HAL-INSU Marine Environmental Research 170 105438 |