An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas
[Departement_IRSTEA]Eaux [ADD1_IRSTEA]Systèmes aquatiques soumis à des pressions multiples International audience Frequent occurrences of pesticides in the environment have raised concerns that combined exposure to these chemicals may result in enhanced toxicity through additive or synergistic inter...
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ftinsu:oai:HAL:hal-03066007v1 2023-06-18T03:40:18+02:00 An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas Mai, Huong Cachot, Jérôme Clérandeau, Christelle Caroline, Martin Mazzella, Nicolas Gonzalez, Patrice Morin, Bénédicte Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes aquatiques et changements globaux (UR EABX) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) 2020-02 https://hal.inrae.fr/hal-03066007 https://hal.inrae.fr/hal-03066007/document https://hal.inrae.fr/hal-03066007/file/Mai%20et%20al,%20ESPR%20version%20finale%20%281%29.pdf https://doi.org/10.1007/s11356-018-3586-6 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-018-3586-6 hal-03066007 https://hal.inrae.fr/hal-03066007 https://hal.inrae.fr/hal-03066007/document https://hal.inrae.fr/hal-03066007/file/Mai%20et%20al,%20ESPR%20version%20finale%20%281%29.pdf doi:10.1007/s11356-018-3586-6 WOS: 000519703100007 info:eu-repo/semantics/OpenAccess ISSN: 0944-1344 EISSN: 1614-7499 Environmental Science and Pollution Research https://hal.inrae.fr/hal-03066007 Environmental Science and Pollution Research, 2020, 27 (4), pp.3600-3611. ⟨10.1007/s11356-018-3586-6⟩ Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2020 ftinsu https://doi.org/10.1007/s11356-018-3586-6 2023-06-05T22:05:14Z [Departement_IRSTEA]Eaux [ADD1_IRSTEA]Systèmes aquatiques soumis à des pressions multiples International audience Frequent occurrences of pesticides in the environment have raised concerns that combined exposure to these chemicals may result in enhanced toxicity through additive or synergistic interaction between compounds. Spermatozoa and embryos of the Pacific oyster, Crassostrea gigas, were exposed to different concentrations of a pesticide mixture with and without copper, mimicking the cocktail of pollutants occurring in the oyster culture area of Arcachon Bay. For the 1× exposure condition, measured concentration corresponds to a total concentration of 1.083 μg L−1 for the mixture of 14 pesticides and to 6.330 μg L−1 for copper (Cu). Several endpoints including larval abnormalities, DNA damage to spermatozoa and embryo and gene expression in D-larvae were investigated. Results demonstrated that pesticide mixtures in combination with or without copper induced a dose-dependent increase in embryotoxic and genotoxic effects on D-larvae from the lowest tested dose of 0.1×. Transcription of genes involved in anti-oxidative stress (cat), respiratory chain (coxI), metal detoxification (mt1 and mt2), and cell cycle arrest and apoptosis (p53) was found to be significantly downregulated while the xenobiotic biotransformation gene gst was significantly upregulated in embryos exposed to pesticide mixture with and without Cu. These findings raise the question of the possible impacts of mixtures of pesticides and metals on wild or farmed oyster populations from polluted coastal marine areas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Environmental Science and Pollution Research 27 4 3600 3611 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster [SDE]Environmental Sciences |
spellingShingle |
Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster [SDE]Environmental Sciences Mai, Huong Cachot, Jérôme Clérandeau, Christelle Caroline, Martin Mazzella, Nicolas Gonzalez, Patrice Morin, Bénédicte An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
topic_facet |
Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster [SDE]Environmental Sciences |
description |
[Departement_IRSTEA]Eaux [ADD1_IRSTEA]Systèmes aquatiques soumis à des pressions multiples International audience Frequent occurrences of pesticides in the environment have raised concerns that combined exposure to these chemicals may result in enhanced toxicity through additive or synergistic interaction between compounds. Spermatozoa and embryos of the Pacific oyster, Crassostrea gigas, were exposed to different concentrations of a pesticide mixture with and without copper, mimicking the cocktail of pollutants occurring in the oyster culture area of Arcachon Bay. For the 1× exposure condition, measured concentration corresponds to a total concentration of 1.083 μg L−1 for the mixture of 14 pesticides and to 6.330 μg L−1 for copper (Cu). Several endpoints including larval abnormalities, DNA damage to spermatozoa and embryo and gene expression in D-larvae were investigated. Results demonstrated that pesticide mixtures in combination with or without copper induced a dose-dependent increase in embryotoxic and genotoxic effects on D-larvae from the lowest tested dose of 0.1×. Transcription of genes involved in anti-oxidative stress (cat), respiratory chain (coxI), metal detoxification (mt1 and mt2), and cell cycle arrest and apoptosis (p53) was found to be significantly downregulated while the xenobiotic biotransformation gene gst was significantly upregulated in embryos exposed to pesticide mixture with and without Cu. These findings raise the question of the possible impacts of mixtures of pesticides and metals on wild or farmed oyster populations from polluted coastal marine areas. |
author2 |
Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes aquatiques et changements globaux (UR EABX) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
format |
Article in Journal/Newspaper |
author |
Mai, Huong Cachot, Jérôme Clérandeau, Christelle Caroline, Martin Mazzella, Nicolas Gonzalez, Patrice Morin, Bénédicte |
author_facet |
Mai, Huong Cachot, Jérôme Clérandeau, Christelle Caroline, Martin Mazzella, Nicolas Gonzalez, Patrice Morin, Bénédicte |
author_sort |
Mai, Huong |
title |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_short |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_full |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_fullStr |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_sort |
environmentally realistic pesticide and copper mixture impacts embryonic development and dna integrity of the pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.inrae.fr/hal-03066007 https://hal.inrae.fr/hal-03066007/document https://hal.inrae.fr/hal-03066007/file/Mai%20et%20al,%20ESPR%20version%20finale%20%281%29.pdf https://doi.org/10.1007/s11356-018-3586-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0944-1344 EISSN: 1614-7499 Environmental Science and Pollution Research https://hal.inrae.fr/hal-03066007 Environmental Science and Pollution Research, 2020, 27 (4), pp.3600-3611. ⟨10.1007/s11356-018-3586-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-018-3586-6 hal-03066007 https://hal.inrae.fr/hal-03066007 https://hal.inrae.fr/hal-03066007/document https://hal.inrae.fr/hal-03066007/file/Mai%20et%20al,%20ESPR%20version%20finale%20%281%29.pdf doi:10.1007/s11356-018-3586-6 WOS: 000519703100007 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s11356-018-3586-6 |
container_title |
Environmental Science and Pollution Research |
container_volume |
27 |
container_issue |
4 |
container_start_page |
3600 |
op_container_end_page |
3611 |
_version_ |
1769005277878681600 |